Advances in Metabolic Engineering of Saccharomyces cerevisiae for the Production of Industrially and Clinically Important Chemicals

Turanlı-Yıldız, Burcu; Hacısalihoğlu, Burcu; Çakar, Z. Petek

doi:10.5772/intechopen.70327

Cited by 6 publications

(5 citation statements)

References 87 publications

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“…There are different genetic engineering approaches that have been employed for yeast improvement for bioethanol production. These include rational metabolic engineering which focuses on the enhancement of the biosynthetic pathways of fermentation enzymes and transporter/regulatory proteins (Turanlı‐Yıldız et al, 2018), gene editing, protein engineering, whole‐genome sequencing, and so on. Another method involves the inverse metabolic engineering under which fermenting yeast genotypes are identified and improved for better production (Nevoigt, 2008; Pena et al, 2018).…”

Section: Strategies For Developing Viable Yeast Strains For Ethanol P...mentioning

confidence: 99%

Genetics and metabolic engineering of yeast strains for efficient ethanol production

Adebami

Kuila

Ajunwa

et al. 2021

J Food Process Engineering

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Bioethanol production from monomeric sugar is performed by several yeasts. But there are several limitations associated with yeast strains such as their low tolerance to ethanol, toxic inhibitors, and high sugar concentration. Genetic and metabolic engineering of potential yeast strains can overcome the above limitations. The present article summarized current genetic and metabolic engineering approaches for the development of yeast strain for efficient ethanol production. The review systematically examined bioethanol generations based on substrate utilization, criteria for strain selections, strategies for strain improvements including randomized mutagenesis, genetic engineering, metabolic engineering, genome editing, whole genome (re)sequencing, promoter engineering, quantitative trait locus analysis, protein engineering, and evolutionary engineering. Different fermentation technologies employed in hydrolysate fermentation including low gravity (LG), high gravity (HG), and very high gravity (VHG) as well as challenges of yeast strains development and its future prospect have been critically evaluated in this article. Significant engineering efforts are imminent for yeast‐based second‐generation biofuel to leave a demonstration phase through strain improvement and become economically competitive with fossil fuel. Practical Applications This is a comprehensive review of yeast strain development for bioethanol production. The readers should be able to acquire some basic knowledge on: The accompanied substrates for bioethanol generations as well as the technologies and challenges behind them. The criteria to consider in selecting yeast strain for bioengineering development. Different strategies and their reported applications employed in yeast strain development including randomized mutagenesis, genetic engineering, metabolic engineering, genome editing, whole genome (re)sequencing, promoter engineering, quantitative trait locus (QTL) analysis, protein engineering, and evolutionary engineering. Challenges and merits of different fermentation technologies employed in hydrolysate fermentation including LG, HG, and VHG. Possible challenges to encounter in developing yeast strain for bioethanol production. Desirable traits to consider in the selection and development of yeast strains for bioethanol production.

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Section: Strategies For Developing Viable Yeast Strains For Ethanol P...mentioning

confidence: 99%

Genetics and metabolic engineering of yeast strains for efficient ethanol production

Adebami

Kuila

Ajunwa

et al. 2021

J Food Process Engineering

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“…Saccharomyces cerevisiae is a widely used microbial cell factory to produce recombinant proteins, natural and unnatural products, biofuels and biochemicals [ 1 , 2 ]. Construction of biosynthetic pathways often requires fine regulation of the expression of multiple genes to balance the intricate metabolic pathway and to achieve high yield for desired products.…”

Section: Introductionmentioning

confidence: 99%

A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

Deng

Zheng

et al. 2021

Microb Cell Fact

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Background Saccharomyces cerevisiae is an important synthetic biology chassis for microbial production of valuable molecules. Promoter engineering has been frequently applied to generate more synthetic promoters with a variety of defined characteristics in order to achieve a well-regulated genetic network for high production efficiency. Galactose-inducible (GAL) expression systems, composed of GAL promoters and multiple GAL regulators, have been widely used for protein overexpression and pathway construction in S. cerevisiae. However, the function of each element in synthetic promoters and how they interact with GAL regulators are not well known. Results Here, a library of synthetic GAL promoters demonstrate that upstream activating sequences (UASs) and core promoters have a synergistic relationship that determines the performance of each promoter under different carbon sources. We found that the strengths of synthetic GAL promoters could be fine-tuned by manipulating the sequence, number, and substitution of UASs. Core promoter replacement generated synthetic promoters with a twofold strength improvement compared with the GAL1 promoter under multiple different carbon sources in a strain with GAL1 and GAL80 engineering. These results represent an expansion of the classic GAL expression system with an increased dynamic range and a good tolerance of different carbon sources. Conclusions In this study, the effect of each element on synthetic GAL promoters has been evaluated and a series of well-controlled synthetic promoters are constructed. By studying the interaction of synthetic promoters and GAL regulators, synthetic promoters with an increased dynamic range under different carbon sources are created.

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“…In the environmental area, biosurfactants are used due to their ability to assist in the treatment of sites contaminated with metals and organic compounds such as hydrocarbons (Kreling et al, 2018;Santos, Rufino, Luna, Santos, & Sarubbo, 2016;Usman, Dadrasnia, Lim, Mahmud, & Ismail, 2016;Yang et al, 2016). When applied to soil bioreme-diation, biosurfactants increase the solubility of contaminants, making them more available for the degradation processes by microorganisms (Decesaro et al, 2017;Mnif, Ellouz-Chaabouni, & Ghribi, 2018).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Among the microorganisms that produce biosurfactants, Saccharomyces cerevisiae yeast is characterized by industrial applicability already consolidated in industrial fermentation processes, such as bread, wine, and beer (Turanlı‐Yıldız, Hacısalihoğlu, & Çakar, 2017). Although these microorganisms have been studied for their potential intracellular biosurfactants production, few studies are reported in the literature, especially related to their use in the production of extracellular biosurfactants.…”

Section: Introductionmentioning

confidence: 99%

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Increasing the production of extracellular biosurfactants from Saccharomyces cerevisiae

Zaparoli

Kreling

Colla

2020

Environmental Quality Mgmt

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Biosurfactants possess surface and interfacial activity, low toxicity, high biodegradability, and stability in wide ranges of pH and temperature, which makes them competitive against the existing synthetic surfactants. However, economic aspects are still a barrier to the application in industrial scale. In order to optimize the production processes, the aim was to evaluate the production of extracellular biosurfactants from Saccharomyces cerevisiae using cellular stress techniques. The yeast was tested for six different stress conditions: ethanol, hydrogen peroxide, pH, temperature, ultraviolet radiation, and freezing/thawing. After exposure to different levels of stress, the biosurfactant production was verified from the formation of stable emulsions and reduction of the surface tension of the culture medium. Results showed a higher resistance of the yeast to stressors, being the highest yields found in ultraviolet radiation and hydrogen peroxide, reaching 1.39 and 1.16 UE d −1 , respectively. Highest surface tension reduction (11 mN m −1 d −1 in 1 d of fermentation) was obtained for the strain stressed by ethanol at 50%. A positive influence was observed in the use of stress techniques over productivity, indicating the production of extracellular biosurfactants, which could low the costs with the downstream process of these bioproducts.

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Advances in Metabolic Engineering of Saccharomyces cerevisiae for the Production of Industrially and Clinically Important Chemicals

Cited by 6 publications

References 87 publications

Genetics and metabolic engineering of yeast strains for efficient ethanol production

Genetics and metabolic engineering of yeast strains for efficient ethanol production

A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

Increasing the production of extracellular biosurfactants from Saccharomyces cerevisiae

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