Biosynthetic gold nanoparticles of Hibiscus syriacus L. callus potentiates anti-inflammation efficacy via an autophagy-dependent mechanism

Xu, Xing; Tran, Thi Hoa; Perumalsamy, Haribalan; Dhandapani, Sanjeevram; Kim, Yeon-Ju

doi:10.1016/j.msec.2021.112035

Cited by 32 publications

(17 citation statements)

References 60 publications

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“…The downregulation of these inflammatory factors also suggested the BRB-AuNCs effectively promoted the conversion of macrophages/microglia from M1 to M2, which reduced the inflammatory response after SCI. Various nanomaterials, such as gold nanoparticles (AuNPs) were not only used as nano-carriers for anti-inflammatory drugs but also had anti-inflammatory activity themselves [ 27–29 ]. The combination of AuNPs and drugs can significantly improve efficiency because it enhances the biocompatibility of drugs and exerts a synergistic effect.…”

Section: Resultsmentioning

confidence: 99%

Gold nanoclusters conjugated berberine reduce inflammation and alleviate neuronal apoptosis by mediating M2 polarization for spinal cord injury repair

Zhou

Fan

et al. 2021

Regenerative Biomaterials

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Spinal cord injury (SCI) leads to nerve cell apoptosis and loss of motor function. Herein, excessive activation of the M1 phenotype macrophages/microglia is found to be the main reason for the poor prognosis of SCI, but the selective activation phenotype (M2) macrophages/microglia facilitates the recovery of SCI. Thereafter, we used gold nanoclusters loaded berberine (BRB-AuNCs) to reduce inflammation by inhibiting the activation of M1 phenotype macrophages/microglia, which simultaneously inhibited neuronal apoptosis after SCI. In vitro and in vivo experiments showed that BRB-AuNCs reduced M1 protein marker CD86, increased M2 protein marker CD206, reduced inflammation and apoptotic cytokines (IL-1β, IL-6, TNF-α, Cleaved Caspase-3, Bax). These results indicate that BRB-AuNCs have excellent anti-inflammatory and anti-apoptotic effects by inducing the polarization of macrophages/microglia from M1 phenotype to M2 phenotype. Thereafter, the motor functions of SCI rats were significantly improved after treatment with BRB-AuNCs. This work not only provides a new way for the treatment of SCI but also broadens BRB utilization strategies.

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Section: Resultsmentioning

confidence: 99%

Gold nanoclusters conjugated berberine reduce inflammation and alleviate neuronal apoptosis by mediating M2 polarization for spinal cord injury repair

Zhou

Fan

et al. 2021

Regenerative Biomaterials

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“…The cells were incubated in 5% CO 2 atmosphere at 37 °C. An MTT test was performed to check the cytotoxicity of the KP-SeNPs on the cells according to a previously reported method [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…KP-SeNPs (1.0 g) and KP-RE (1.0 g) was mixed with 5% DMSO/MeOH, respectively, and filtered using 0.22 μm syringe filters (ADVANTEC, Tokyo, Japan). The LC analysis was performed on an Ultimate 3000 system (Thermo Scientific, USA) according to previously reported methods [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Biologically synthesized black ginger-selenium nanoparticle induces apoptosis and autophagy of AGS gastric cancer cells by suppressing the PI3K/Akt/mTOR signaling pathway

Wang

Dhandapani

et al. 2022

J Nanobiotechnol

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Background Despite being a promising strategy, current chemotherapy for gastric cancer (GC) is limited due to adverse side effects and poor survival rates. Therefore, new drug-delivery platforms with good biocompatibility are needed. Recent studies have shown that nanoparticle-based drug delivery can be safe, eco-friendly, and nontoxic making them attractive candidates. Here, we develop a novel selenium-nanoparticle based drug-delivery agent for cancer treatment from plant extracts and selenium salts. Results Selenium cations were reduced to selenium nanoparticles using Kaempferia parviflora (black ginger) root extract and named KP-SeNP. Transmission electron microscopy, selected area electron diffraction, X-ray diffraction, energy dispersive X-ray, dynamic light scattering, and Fourier-transform infrared spectrum were utilized to confirm the physicochemical features of the nanoparticles. The KP-SeNPs showed significant cytotoxicity in human gastric adenocarcinoma cell (AGS cells) but not in normal cells. We determined that the intracellular signaling pathway mechanisms associated with the anticancer effects of KP-SeNPs involve the upregulation of intrinsic apoptotic signaling markers, such as B-cell lymphoma 2, Bcl-associated X protein, and caspase 3 in AGS cells. KP-SeNPs also caused autophagy of AGS by increasing the autophagic flux-marker protein, LC3B-II, whilst inhibiting autophagic cargo protein, p62. Additionally, phosphorylation of PI3K/Akt/mTOR pathway markers and downstream targets was decreased in KP-SeNP-treated AGS cells. AGS-cell xenograft model results further validated our in vitro findings, showing that KP-SeNPs are biologically safe and exert anticancer effects via autophagy and apoptosis. Conclusions These results show that KP-SeNPs treatment of AGS cells induces apoptosis and autophagic cell death through the PI3K/Akt/mTOR pathway, suppressing GC progression. Thus, our research strongly suggests that KP-SeNPs could act as a novel potential therapeutic agent for GC. Graphical Abstract

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“…Recent studies suggested that nanotechnology can be used to prevent and treat inflammatory diseases. Various nano-sized materials, particularly nanoparticles, have been studied to determine their roles in inflammatory responses. , These materials can improve bioavailability and drug delivery at the inflammation site while causing few side effects and have a good safety profile . Several researchers have proposed using nanoparticles, such as polymeric nanoparticles, metallic nanoparticles, lipid nanoparticles, and vesicular systems, because of their stable, safe, and target-specific delivery characteristics for treating numerous disorders. , In recent years, plant-based metallic nanoparticles produced using green synthesis methods have been explored to optimize the conventional efficacy of the original plants based on their advantages, including their high biocompatibility, low cost, and eco-friendly nature. − Nevertheless, further research on metallic nanoparticles synthesized from various types of plants is needed to develop anti-inflammatory candidates.…”

Section: Introductionmentioning

confidence: 99%

Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-κB Signaling Pathway in TNF-α/IFN-γ-Induced HaCaT Keratinocytes

et al. 2022

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Nanotechnology-applied materials and related therapeutics have gained attention for treating inflammatory skin diseases. The beach rose (Rosa rugosa), belonging to the family Rosaceae, is a perennial, deciduous woody shrub endemic to northeastern Asia. In this study, R. rugosa-based gold nanoparticles (RR-AuNPs) were biologically synthesized under optimal conditions to explore their potential as anti-inflammatory agents for treating skin inflammation. The synthesized RR-AuNPs were analyzed using field emission-transmission electron microscopy, energy-dispersive X-ray spectrometry, selected-area electron diffraction, and X-ray diffraction. The uniformly well-structured AuNPs showed near-spherical and polygonal shapes. Cell viability evaluation and optical observation results showed that the RR-AuNPs were absorbed by human keratinocytes without causing cytotoxic effects. The effects of RR-AuNPs on the skin inflammatory response were investigated in human keratinocytes treated with tumor necrosis factor-α/interferon-γ (T + I). The results showed that T + I-stimulated increases in inflammatory mediators, including chemokines, interleukins, and reactive oxygen species, were significantly suppressed by RR-AuNP treatment in a concentration-dependent manner. The western blotting results indicated that the RR-AuNP-mediated anti-inflammatory effects were highly associated with the suppression of inflammatory signaling, mitogen-activated protein kinase, and nuclear factor-κB. These results demonstrate that plant extract-based AuNPs are novel anti-inflammatory candidates for topical application to treat skin inflammation.

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Biosynthetic gold nanoparticles of Hibiscus syriacus L. callus potentiates anti-inflammation efficacy via an autophagy-dependent mechanism

Cited by 32 publications

References 60 publications

Gold nanoclusters conjugated berberine reduce inflammation and alleviate neuronal apoptosis by mediating M2 polarization for spinal cord injury repair

Gold nanoclusters conjugated berberine reduce inflammation and alleviate neuronal apoptosis by mediating M2 polarization for spinal cord injury repair

Biologically synthesized black ginger-selenium nanoparticle induces apoptosis and autophagy of AGS gastric cancer cells by suppressing the PI3K/Akt/mTOR signaling pathway

Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-κB Signaling Pathway in TNF-α/IFN-γ-Induced HaCaT Keratinocytes

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