Biosynthesis of silver nanoparticles and their versatile antimicrobial properties

Some, Sudip; Sen, Ipsita K.; Mandal, Amit Kumar; Aslan, Tuğrul; Üstün, Yakup; Yılmaz, Ebru Şebnem; Katı, Ahmet; Demırbas, Ayse; Öçsoy, İsmail

doi:10.1088/2053-1591/aae23e

Cited by 80 publications

(39 citation statements)

References 99 publications

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“…According to the previous reports, flavonoids especially the -OH groups present in flavonoids are responsible for the reduction of silver ions. It has been proposed that hydrogen ions are released during the tautomeric transformation of enol form of flavonoids to keto form, resulting in the reduction of silver ions and synthesis of silver nanoparticles 21,40,53 .…”

Section: Resultsmentioning

confidence: 99%

Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Some

Bulut

Biswas

et al. 2019

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Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

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Section: Resultsmentioning

confidence: 99%

Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Some

Bulut

Biswas

et al. 2019

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“…Therefore alternative strategies to antibiotics are urgently required. While the antibacterial potential of AgNPs has been described ( Morones et al, 2005 ; Li et al, 2011 ; El-Rab, 2012 ; Taglietti et al, 2012 ; Polivkova et al, 2017 ; Saratale et al, 2017 ; Burduşel et al, 2018 ; Kshirsagar et al, 2018 ; Souza et al, 2018 ; Zheng et al, 2018 ; Zhu et al, 2018 ; Some et al, 2019 ), we are unaware of any studies which have examined the antibacterial potential including antibiotic synergistic effects, of biocompatible concentrations of AgNPs. In vitro studies that have assessed the cytotoxicity of AgNPs were either too short, e.g., 24 h or ≤5 days and/or did not consider the antibacterial efficacy ( Patra and Baek, 2017 ; Alsammarraie et al, 2018 ; Botha et al, 2019 ; Samuel et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles at Biocompatible Dosage Synergistically Increases Bacterial Susceptibility to Antibiotics

et al. 2020

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Antibiotics used to treat bacterial infections can become ineffective over time or result in the emergence of antibiotic resistant pathogens. With the advent of nanotechnology, silver nanoparticles (AgNPs) have gained significant attention as a therapeutic agent due to the well-known antimicrobial properties of silver. However, there are concerns and limited literature on the potential cytotoxicity of nanoparticles at effective antimicrobial concentrations. AgNPs prepared from silver nitrate with glucose reduction were characterized by surface plasmon resonance, dynamic light scattering, zeta potential analysis and transmission electron microscopy. The cytotoxicity of AgNPs towards human gingival fibroblasts over 7 days was determined using cell proliferation assays and confocal microscopy. AgNP MIC and antibacterial effects alone and in combination with 11 antibiotics were determined against a panel of nine microbial species including gram-positive and gram-negative bacterial species. AgNPs concentrations ≤ 1 µg/mL were non-cytotoxic but also showed no antibacterial effects. However, when combined with each of eleven antibiotics, the biocompatible concentration of AgNPs (1 µg/mL) resulted in significant inhibition of bacterial growth for multiple bacterial species that were resistant to either the antibiotics or AgNPs alone. This study presents a promising strategy with further testing in vivo, to develop novel antimicrobial agents and strategies to confront emerging antimicrobial resistance.

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“…Plants during glycolysis produce a large amount of H + ions along with NAD which acts as a strong reducing agent; this seems to be responsible for the formation of AgNPs [79]. Some et al [80] reviewed the huge importance of modulating metals into solid nanoparticles (SNPs) which offer new scientific and industrial applications, including biomedical products based on P. amarus. Pointing out some advantages of AgNPs, they have been used as i) safe preservatives due to non-penetrating property to human skin, ii) in agriculture control an important crop pest and combat several herb diseases, iii) in chemical industry it preserves the environment acting against pesticide contamination by mineralization, iv) in biomedicine acts as antiviral, anti-inflammatory, anticancer, and antimicrobial (B. subtilis, S. aureus, P. aeruginosa, P. pneumonia, and S. Flexneri) [80].…”

Section: Acsr000510 1(2)2019mentioning

confidence: 99%

Botanic, Phytochemistry and Pharmacological Aspects of Phyllanthus Amarus Schum. & Thonn. as Powerful Tools to Improve its Biotechnological Studies

Aparecida¹

2019

ACSR

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Phyllanthus (Euphorbiaceae) is a native genus of the American continent representative medicinal plant with very high number of species (more than 1,000) located in tropical and subtropical countries. In Brazil, this genus is represented by over than 100 specimens, that are widely distributed in different environments. In fact, Phyllanthus is present in almost all Brazilian territories. The species of this genus are extremely used in folk traditional medicine. The species Phyllanthus amarus Schum. & Thonn. and P. niruri L. have been frequent targets of ethnopharmacological work. However, in the beginning of this century botanic studies were intensified by using molecular markers for unambiguously identification of Phyllanthus species and to indicate the significant differences between the specimens of this genus. There are many differences between P. amarus and P. niruri, and the main of them were found to be in base, seed and stigma type. Systematic studies at molecular level have been assessed for acute authentication of plant specimen by using RAPD, ISSR, SCAR, AFLP, ITS, RFLP, ISSR-PCR and PCR-RFLP-ITS markers linked to a dominant gene. These methods have been assessed to the genus Phyllanthus with highly efficient results. With respect to phytocomponents of P. amarus herb, it is proved that the lignans Phyllanthin and hypophyllanthin are the main biomarkers. Focusing in one example phytochemical investigations were conducted with an unambiguous identified P. amarus collected from Brazil. Its ethanolic extract, revealed the presence of six bioactive lignans (phyllanthin, hypophyllanthin, isolintetralin, demethylenedioxyniranthin, 5-demethoxy-niranthin, niranthin) and one triterpene (2Z, 6Z, 10Z, 14E, 18E, 22E-farnesilfarnesol). This rich lignan extract was used in nanoemulsion formulations, an efficient therapeutic medicine to be applied in the health care. Other polar extracts of P. amarus have been encapsulated by using bio-nanotechnologies and are presented in this review. Technological innovations and recent progress in the multiple aspects of Phyllanthus´s plant development was reviewed. Today's current state of Phyllanthus amarus Schum. & Thonn. is supported by its scientific findings, which has many applications, of which mainly focusing in the biotechnology field to face the new challenges for this lead medicinal plant.

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Biosynthesis of silver nanoparticles and their versatile antimicrobial properties

Cited by 80 publications

References 99 publications

Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Silver Nanoparticles at Biocompatible Dosage Synergistically Increases Bacterial Susceptibility to Antibiotics

Botanic, Phytochemistry and Pharmacological Aspects of Phyllanthus Amarus Schum. & Thonn. as Powerful Tools to Improve its Biotechnological Studies

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