Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Abstract

Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

Light Tailoring: Impact of UV-C Irradiation on Biosynthesis, Physiognomies, and Clinical Activities of Morus macroura-Mediated Monometallic (Ag and ZnO) and Bimetallic (Ag–ZnO) Nanoparticles

“…The peaks at 3300-3500 cm −1 reflect strong N-H stretching in alcohols as well as phenolic and flavonoid compounds. The peaks at 1627.73 cm −1 and 1635.44 cm −1 correspond to carbonyl stretching frequency, as reported by Some et al at 1630 cm −1 for AgNPs synthesized from Morus indica leaf extract [33]. The FTIR spectrum of AgNPs-and M. macroura leaf extract revealed strong interactions with the fruit extract during their synthesis.…”

Green synthesis and characterization of copper nanoparticles using Piper retrofractum Vahl extract as bioreductor and capping agent

“…The -OH groups present in flavonoids are responsible for the reduction of nanoparticles. It has been proposed that hydrogen ions are released during the tautomeric transformation of the enol form of flavonoids to keto form, resulting in the reduction of metal ions and formation of metal nanoparticles [34]. In this study, the total phenolic content of Piper retrofractum extract was approximately about 0.98 mg GAE/g.…”

Untitled

“…The extracts exhibited significant dose-dependent inhibition of growth on HT-29 cells with IC 50 values of 169.7 µg/ml (MV1ME) and 55.01 µg/ml (MV1A) respectively. Some S et al, (2019) 29 , reported maximum cytotoxicity of 41% and 49% by MV1 aqueous extract based silver nanoparticles at 250µg/ml concentration on HepG2 and WRL-68 human cancer cell lines respectively with a IC 50 higher than 250 µg/ml. In our study, the aqueous extract of MV1 showed high cytotoxic effect on colon cancer cell line with very low IC 50 value.…”