Biosynthesis of riboflavin

Bacher, Adelbert; Eberhardt, Sabine; Eisenreich, Wolfgang; Fischer, Markus; Herz, Stefan; Illarionov, Boris; Kis, Klaus; Richter, Gerald

doi:10.1016/s0083-6729(01)61001-x

Cited by 120 publications

(101 citation statements)

References 157 publications

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“…Assuming no recovery of energy from pyrophosphate produced during biosynthesis, 1 mol of riboflavin could require as much as 25 mol ATP to produce (19,20). To accumulate to 250 nM within a 72-h period would be equivalent to an ATP cost as high as 6.7 ϫ 10 Ϫ3 mol ATP⅐mg protein Ϫ1 ⅐h Ϫ1 .…”

Section: Resultsmentioning

confidence: 99%

Shewanella secretes flavins that mediate extracellular electron transfer

Marsili¹,

Baron²,

Shikhare³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

1,706

1,507

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Bacteria able to transfer electrons to metals are key agents in biogeochemical metal cycling, subsurface bioremediation, and corrosion processes. More recently, these bacteria have gained attention as the transfer of electrons from the cell surface to conductive materials can be used in multiple applications. In this work, we adapted electrochemical techniques to probe intact biofilms of Shewanella oneidensis MR-1 and Shewanella sp. MR-4 grown by using a poised electrode as an electron acceptor. This approach detected redox-active molecules within biofilms, which were involved in electron transfer to the electrode. A combination of methods identified a mixture of riboflavin and riboflavin-5 -phosphate in supernatants from biofilm reactors, with riboflavin representing the dominant component during sustained incubations (>72 h). Removal of riboflavin from biofilms reduced the rate of electron transfer to electrodes by >70%, consistent with a role as a soluble redox shuttle carrying electrons from the cell surface to external acceptors. Differential pulse voltammetry and cyclic voltammetry revealed a layer of flavins adsorbed to electrodes, even after soluble components were removed, especially in older biofilms. Riboflavin adsorbed quickly to other surfaces of geochemical interest, such as Fe(III) and Mn(IV) oxy(hydr)oxides. This in situ demonstration of flavin production, and sequestration at surfaces, requires the paradigm of soluble redox shuttles in geochemistry to be adjusted to include binding and modification of surfaces. Moreover, the known ability of isoalloxazine rings to act as metal chelators, along with their electron shuttling capacity, suggests that extracellular respiration of minerals by Shewanella is more complex than originally conceived.bioelectrochemistry ͉ biogeochemistry ͉ redox mediator ͉ riboflavin

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Section: Resultsmentioning

confidence: 99%

Shewanella secretes flavins that mediate extracellular electron transfer

Marsili¹,

Baron²,

Shikhare³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

1,706

1,507

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“…Fermentation processes are progressively replacing chemical manufacturing processes because of a significant cost reduction, and because they meet the "green" demand for replacing a mineral-oil-based process with one based on renewable resources (Stahmann et al, 2000;Van Loon et al, 1996). This development was initially prompted by the natural occurrence of bacteria, yeasts, and fungi, which produce riboflavin in levels exceeding their apparent metabolic requirements (Bacher et al, 2000). The key to the current competitiveness has been decades of strain development.…”

Section: Industrial Production Of Riboflavin (Vitamin B2)mentioning

confidence: 99%

Scale-down and parallel operation of the riboflavin production process with Bacillus subtilis

Knorr

Schlieker²,

Hohmann³

et al. 2007

Biochemical Engineering Journal

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“…19 Riboflavin is synthesised by many bacteria and its biosynthetic pathway has been studied extensively in B. subtilis and E. coli. 20,21 This study highlights the microbial diversity of riboflavin producing strains isolated from various sources. The findings suggest that the Lactobacilli isolated from human feces and fermented bamboo shoots have shown maximum riboflavin production as compared to isolates of dairy origin.…”

Section: 14mentioning

confidence: 98%

Exploring Indigenous Lactobacillus Species from Diverse Niches for Riboflavin Production

Thakur¹,

Tomar²

2015

JYP

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Background: Riboflavin has received rather less attention in the past years, but interest is increasing because it is recognized as an essential component of cellular biochemistry, it is one of the vital vitamins required by human, bacteria, animals and plants. Today, bio production of riboflavin is competing with chemically synthesized one. Lactobacilli are well reckoned for their technological and probiotic significance and their exercise in dairy products for trade and industry. Materials and Methods: The present study was carried out to isolate and characterize riboflavin lactobacilli from various dairy and non-dairy sources of Indian origin. The isolates were subjected to phenotypic and genotypic characterizations followed by partial 16S rDNA sequencing. Results: As many as 40 isolates were identified as Lactobacilli and among them 14 were identified as L.plantarum, 5 as L. fermentum, 5 as L. delbrueckii subsp. bulgaricus, 4 L. brevis, 2 L. pentosus and 1 isolate as L. mucosae. Further isolates were screened for riboflavin production by chemical assay method and among all the tested isolates, 2 isolates viz. KTLF1 (L.fermentum) and KTLP13 (L.planatrum) have been observed to produce appreciable amount of riboflavin in MRS and RAM. Conclusion: These riboflavin producing lactobacilli can be used as a model for the development of strains that have the potential to produce an essential vitamin in situ which would contribute significantly to the functional value of certain fermented foods.

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Biosynthesis of riboflavin

Cited by 120 publications

References 157 publications

Shewanella secretes flavins that mediate extracellular electron transfer

Shewanella secretes flavins that mediate extracellular electron transfer

Scale-down and parallel operation of the riboflavin production process with Bacillus subtilis

Exploring Indigenous Lactobacillus Species from Diverse Niches for Riboflavin Production

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