Biosynthesis of Proteoglycans: An Approach to Locate it in Different Membrane Systems

To, Kleine

doi:10.1016/b978-0-12-363709-3.50008-5

Cited by 14 publications

(3 citation statements)

References 421 publications

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“…This is in agreement with the staining pattern of the dye monomers, which consists of the tightest network of filaments occasionally forming fine lateral branches. Further support for this hypothesis comes from the comparison of their dimensions with the proteoglycan aggregates isolated from the hyaline cartilage (Hascall, 1980, Kleine, 1981, Rosenberg et al, 1975, Thyberg, 1977. The filaments correspond in size to the proteoglycan monomers, while the very fine lateral branches are probably glycosaminoglycans not completely collapsed on the protein core.…”

Section: Discussionmentioning

confidence: 83%

The reaction of Acridine Orange with proteoglycans in the articular cartilage of the rat

Brandes¹,

Reale²

1990

Histochem J

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Acridine Orange in concentrations from 0.01% to 0.2% was added to the first fixative solution in order to stain vibratome sections and small blocks of the articular cartilage of 2 month old rats. The interterritorial matrix of the radial or deep zone (zone 3) was examined. It contained reaction products with different morphology depending on the specimens used. In vibratome sections filaments were seen arranged in a homogenous pattern and changing in size with the concentration of the dye: diluted solutions produced finer filaments than concentrated ones. In contrast, in tissue blocks the staining pattern was not altered by different concentrations of Acridine Orange. However, with increase of the distance from the surface of the specimens the size of the filaments gradually decreased and formed a finer network. Since after preincubation with chondroitin ABC lyase only minute reaction products remained, an interaction of the dye with the sulphated glycosaminoglycans of the proteoglycans in the articular cartilage is suggested. The experiments show that by using mainly monocationic monomers of Acridine Orange the proteoglycans can be stained in a more expanded state than with polycationic dye polymers.

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Section: Discussionmentioning

confidence: 83%

The reaction of Acridine Orange with proteoglycans in the articular cartilage of the rat

Brandes¹,

Reale²

1990

Histochem J

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“…Although a common amino acid as glutamic acid and a prominent constituent of many glycosaminoglycans [94], free glutamate appears to be very toxic to central nervous system neurons, especially those communicating through graded potentials. When liberated at the external plexiform layer (first synapse away from photoreceptors), it must be cleared quickly.…”

Section: Possible Physiological Events In Juvenile Onset Myopia: Neurmentioning

confidence: 99%

Retinal-image mediated ocular growth as a mechanism for juvenile onset myopia and for emmetropization

Goss

Wickham

1995

Doc Ophthalmol

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The very common ocular clinical ocular condition in children juvenile onset myopia results from axial elongation of the eye. In humans, some studies have found an association of myopia with greater levels of nearpoint activity and with differences in accommodation and convergence function. This paper reviews a variety of laboratory and clinical studies which are consistent with the hypothesis that retinal image defocus is biochemically transformed into an axial elongation expressed through increased posterior segment growth, and thus myopia. This paper also reviews theories of emmetropization, and classifies them as correlational, feedback, and combination. Evidence is presented to suggest that a combination theory, which combines both correlation of the ocular dioptric components and some feedback mechanism for growth of the eye, is the most correct. Current laboratory research suggests that quality and/or focus (defocus) of retinal imagery is involved in this feedback mechanism and that experimentally induced myopia might be enhanced, reduced or eliminated by pharmaceutical application. Direction of defocus may affect the rate of posterior segment growth, and thus the rate of ocular axial elongation.

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“…This proteoglycan is produced intracellularly through the Golgi apparatus (Kleine 1981). Hyaluronan, on the other hand, is produced and extruded directly into the extracellula*r space through an enzyme located in the cell wall membrane (Prehm 1989).…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan in the exfoliation syndrome

Fitzsimmons

Wallin

1997

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To histochemically analyze if the glycoseaminoglycan component of pseudoexfoliation material consists of hyaluronan. Methods: The hyaluronan binding region of cartilage proteoglycans was utilized in a staining specific for hyaluronan. The technique has been described and employed previously. Human donor eyes with and without the presence of visible pseudoexfoliations were analyzed. Results: Hyaluronan was found to coat the fibrillar exfoliation material on the lens, the zonules, the iris epithelium and the ciliary body. It was also found on a capsular bag following cataract surgery. Conclusion: In the anterior chamber hyaluronan constitutes one part of the exfoliation material. Abnormal findings of hyaluronan could be found in the cornea.

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Biosynthesis of Proteoglycans: An Approach to Locate it in Different Membrane Systems

Cited by 14 publications

References 421 publications

The reaction of Acridine Orange with proteoglycans in the articular cartilage of the rat

The reaction of Acridine Orange with proteoglycans in the articular cartilage of the rat

Retinal-image mediated ocular growth as a mechanism for juvenile onset myopia and for emmetropization

Hyaluronan in the exfoliation syndrome

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