1990
DOI: 10.1007/bf01885789
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The reaction of Acridine Orange with proteoglycans in the articular cartilage of the rat

Abstract: Acridine Orange in concentrations from 0.01% to 0.2% was added to the first fixative solution in order to stain vibratome sections and small blocks of the articular cartilage of 2 month old rats. The interterritorial matrix of the radial or deep zone (zone 3) was examined. It contained reaction products with different morphology depending on the specimens used. In vibratome sections filaments were seen arranged in a homogenous pattern and changing in size with the concentration of the dye: diluted solutions pr… Show more

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Cited by 15 publications
(8 citation statements)
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“…For in situ pH measurement with an acridine orange fluorescent probe, sections were treated with ferriammonium sulfate for 15 minutes, rinsed in distilled water, and stained with 1 mg/ml of acridine orange (catalog no. A‐6014; Sigma, St. Louis, MO) for 1.5 minutes (17). For purposes of comparison, some sections from grade 0 samples were balanced to different pH values by a 3‐day incubation at pH 4.0–8.0 (acetate buffer for pH 4.0 and 5.0, phosphate buffer for pH 6.0, 7.0, and 8.0, and citrate buffer for pH 4.0, 5.0, 6.0, 7.0, and 8.0).…”
Section: Methodsmentioning
confidence: 99%
“…For in situ pH measurement with an acridine orange fluorescent probe, sections were treated with ferriammonium sulfate for 15 minutes, rinsed in distilled water, and stained with 1 mg/ml of acridine orange (catalog no. A‐6014; Sigma, St. Louis, MO) for 1.5 minutes (17). For purposes of comparison, some sections from grade 0 samples were balanced to different pH values by a 3‐day incubation at pH 4.0–8.0 (acetate buffer for pH 4.0 and 5.0, phosphate buffer for pH 6.0, 7.0, and 8.0, and citrate buffer for pH 4.0, 5.0, 6.0, 7.0, and 8.0).…”
Section: Methodsmentioning
confidence: 99%
“…Frontal sections (12 m) were cut with a cryostat and mounted onto poly-L-lysine-coated object slides. For semithin sectioning, tissue was Wxed with glutaraldehyde (2.5%) in sodium cacodylate (0.1 M, pH 7.3) and osmium tetroxide (2% in sodium cacodylate, 0.1 M) and embedded in Epon (Brandes and Reale 1990;Wewetzer et al 2005). Semithin sections (0.5 m) were cut using an Ultracut E (Reichert-Jung, Wetzlar, Germany) and stained with toluidine blue.…”
Section: Histology and Immunohistochemistrymentioning
confidence: 99%
“…The elongated shape of collagen-associated proteoglycans is also preserved with solutions of the monocationic dye Acridine Orange (reviewed in Brandes & Reale, 1990), or after CPC-precipitation (Malchiodi Albedi et al, 1988) and is well correlated to data from rotary shadowing (Iozzo et al, 1982). Moreover, following high-pressure freezing, freeze substitution and low-temperature embedding, which is assumed to preserve proteoglycans 'in their native extended state', cartilage proteoglycans appear as a 'reticular network' of 'threads' (Hunziker & Schenk, 1987).…”
Section: Discussionmentioning
confidence: 99%