Biosynthesis of polyhydroxyalkanoate (PHA) by a newly isolated strain Bacillus tequilensis ARY86 using inexpensive carbon source

Yasin, Akram R.; Al-Mayaly, Ithar Kamil

doi:10.1016/j.biteb.2021.100846

Cited by 7 publications

(6 citation statements)

References 38 publications

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“…For example, Jiang et al (2012) reported PHA productivity could reach 2 g/L per day when using paper mill wastewater as a substrate. Mohanrasu et al (2020) also reported a 5.61 g/L production concentration by Bacillus megaterium in a batch culture within 64 h. Yasin and Al-Mayaly (2021) reported that Bacillus tequilensis ARY86 produced PHA with a PHA/CDM ratio of 64% and a PHA concentration of 1.66 g/L using 1% lactose as a carbon source in the medium. Thu et al (2021) reported that Pichia sp.…”

Section: -L Scale Pha Production Processmentioning

confidence: 93%

Polyhydroxyalkanoate production from food packaging waste paper

Tu,

Hsiung,

Liang

et al. 2023

BioRes

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This study evaluated the production of marine biodegradable plastics, specifically polyhydroxyalkanoates (PHAs), using waste paper from food containers as a novel material source. The results showed that adding dilute sulfuric acid as a pretreatment may have a negative impact on enzyme hydrolysis efficiency. Without pretreatment, the highest glucose concentration was observed in the 50-min heating group. In the experimental group with 1% dilute sulfuric acid as a pretreatment, the highest average glucose concentration was observed in the 25-min treatment group. In flask scale experiments, the C/N ratio was controlled at 10, 20, and 30. The results showed that when the C/N ratio was 10, the PHA/CDM ratios were 16.3 and 23.6 at 48 and 72 h, respectively. After 96 h of cultivation using hydrolysis liquid from the waste paper container as the sole carbon source in a 5-L scale experiment, the PHA/CDM ratio was 28.7 and the PHA concentration was 0.95 g/L. The potential bacterial strain in this study was confirmed to be a Bacillus genus bacterium after strain identification. The signal peaks indicated that the PHA obtained from the Bacillus sp. production process was PHB.

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Section: -L Scale Pha Production Processmentioning

confidence: 93%

Polyhydroxyalkanoate production from food packaging waste paper

Tu,

Hsiung,

Liang

et al. 2023

BioRes

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“…Figure 2(C) have similar highest peaks which were at 1008 cm -1 that represented the C-O-C chemical groups of the polymer. The peaks between 1636 cm -1 and 1240 cm -1 were due to the presence of the C=O and C-O compared to the FTIR analysis PHA standard from Yasin and Al-Mayaly (11).…”

Section: Ft-ir Analysis Before and After Sample Extractionmentioning

confidence: 93%

“…Direct FTIR scanning was performed on all samples, both before and after extraction. PHA can be detected using FT-IR spectroscopy (11). For HPLC analysis, the samples were eluted with 5 ml C18 110 column and performed in isocratic mode with water/ACN 70:30 at a flow rate of 0.6 mL/min at 25 °C.…”

Section: Statistical and Analytical Methodsmentioning

confidence: 99%

“…The peak was plotted using Agilent Resolutions Pro as shown in Figure 1. The extracted polymer and FTIR peaks obtained were compared to the different results from previous research together with FTIR peaks of the standard PHA (8,11,13). Starting off with the samples from before extraction, the first one was samples from spent oils (Figure 1A).…”

Section: Ft-ir Analysis Before and After Sample Extractionmentioning

confidence: 99%

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Production of Polyhydroxyalkanoates (PHA) by Probiotic Bacteria Bacillus tequilensis for Potentially Used as Drug Carrier

Jamaludin,

Mohd Fuzi,

Mohd Ghazali

et al. 2023

MJMHS

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Introduction: Polyhydroxyalkanoate (PHA) is a biopolymer that can be produced by microorganisms from numerous low-cost carbon sources, making it an environmentally friendly material. This study was designed to utilize different food waste (household food waste, spent oils and spent coffee grounds) as nutrient source for the cultivation of microbes to produce polyhydroxyalkanoate (PHA). Methods: The bacterial strain Bacillus tequilensis was grown in 250 mL Erlenmeyer flask each containing 50 mL of mineral salt medium, 25 ml of nutrient broth inoculum and 20 g/L of household food waste, spent ground coffee and spent oils, respectively. The initial pH of the media was 7.0 and the cultured bacteria was incubated at 30 °C, 180 rpm for 72 h as a batch culture. The sample was then extracted and weight, and further analyzed using Fourier Transform Infrared Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Results: B. tequilensis yielded PHA of 7 % to 8 % (g PHA/g dry cell weight) on average using medium containing household food waste, spent ground coffee and spent oils. FTIR analysis showed the peaks range between 1750-1730 cm-1 which belong to PHA functional groups such as C=O. HPLC chromatogram revealed that the retention time obtained from digested PHA was approximately 4.5 min which was similar to the standard of PHA. Conclusion: This enables the utilization of low-cost waste by probiotic B. tequilensis as a carbon source for the sustainable production of biodegradable PHA for a wide range of applications in medicine.

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“…Afterward, the isothermally crystallized samples were heated again at 1 • C/min. [150,151] X-ray diffraction…”

Section: Crystallinitymentioning

confidence: 99%

Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

2022

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible biopolymers. These biomaterials have grown in importance in the fields of tissue engineering and tissue reconstruction for structural applications where tissue morphology is critical, such as bone, cartilage, blood vessels, and skin, among others. Furthermore, they can be used to accelerate the regeneration in combination with drugs, as drug delivery systems, thus reducing microbial infections. When cells are cultured under stress conditions, a wide variety of microorganisms produce them as a store of intracellular energy in the form of homo- and copolymers of [R]—hydroxyalkanoic acids, depending on the carbon source used for microorganism growth. This paper gives an overview of PHAs, their biosynthetic pathways, producing microorganisms, cultivation bioprocess, isolation, purification and characterization to obtain biomaterials with medical applications such as tissue engineering.

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Biosynthesis of polyhydroxyalkanoate (PHA) by a newly isolated strain Bacillus tequilensis ARY86 using inexpensive carbon source

Cited by 7 publications

References 38 publications

Polyhydroxyalkanoate production from food packaging waste paper

Polyhydroxyalkanoate production from food packaging waste paper

Production of Polyhydroxyalkanoates (PHA) by Probiotic Bacteria Bacillus tequilensis for Potentially Used as Drug Carrier

Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

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