Biosynthesis of poly (γ-glutamic acid) from l-glutamine, citric acid and ammonium sulfate in Bacillus subtilis IFO3335

Kunioka, Masao

doi:10.1007/bf00169951

Cited by 66 publications

(21 citation statements)

References 21 publications

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“…1). Kunioka [13] had observed higher yield of PGA without any by-products by using L-glutamine instead Lglutamic acid. Maximum yield of PGA was obtained by using 0.1 g/l of L-glutamine [13].…”

Section: Resultsmentioning

confidence: 96%

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Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Bajaj

2008

Appl Biochem Biotechnol

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The aim of the present work was to study the effect of addition of different amino acids and tricarboxylic acid cycle intermediates as metabolic precursors on the production of poly (gamma-glutamic acid) (PGA) by Bacillus licheniformis NCIM 2324. A maximum yield of 35.75 g/l was obtained with the medium supplemented with 0.5 mM L-glutamine and 10 mM alpha-ketoglutaric acid as compared to 26.12 g/l PGA achieved with the control in the absence of metabolic precursors. Addition of precursors also enhanced the utilization of L-glutamic acid during fermentation.

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Section: Resultsmentioning

confidence: 96%

“…Kunioka [13] used L-glutamine instead of L-glutamic acid for production of PGA from B. subtilis IFO 3335. Higher yield of PGA without any by-products was observed in the L-glutamine/citric acid medium than in the L-glutamic acid/citric acid medium.…”

mentioning

confidence: 99%

Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Bajaj

2008

Appl Biochem Biotechnol

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“…EVect of amino acid on PGA production in SSF has not yet been studied by any investigators. However, in SmF, Kunioka [15] observed higher yield of PGA without any by-products by using L-glutamine instead L-glutamic acid. Maximum PGA yield was obtained by using 0.1 g/l of L-glutamine, and yield was found to decrease on either side of this concentration [15].…”

Section: Optimization By Rsmmentioning

confidence: 96%

Enhanced production of poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 in solid state fermentation

Bajaj

Lele

2008

J Ind Microbiol Biotechnol

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This work reports on the optimization of PGA production by Bacillus licheniformis NCIM 2324 in solid state fermentation (SSF). In the first step, the one factor-at-a-time method was used to investigate the effect of solid substrates, initial moisture content, pH, and additional carbon and nitrogen source on PGA production; subsequently, response surface methodology (RSM) was used to establish the optimum concentrations of the key nutrients for higher PGA production. In the second step, the effects of amino acids and TCA cycle intermediates on the production of PGA were studied. The final optimized medium gave a maximum yield of 98.64 +/- 1.61 mg gds(-1) of PGA, which is significantly higher than that reported in the literature.

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“…It has broad applications in fields of medicine, foods, plastics, and many others [1]. Production of γ-PGA was most extensively studied, and work has been carried out on the nutritional requirements to increase the yield of γ-PGA [2][3][4][5]. Glycerol has been reported to be an important nutriment in γ-PGA production in Bacillus licheniformis ATCC 9945a, a famous γ-PGA producer, as it could obviously increase the yield of γ-PGA [5].…”

Section: Introductionmentioning

confidence: 99%

Contribution of Glycerol on Production of Poly(γ-Glutamic Acid) in Bacillus subtilis NX-2

Liang

et al. 2008

Appl Biochem Biotechnol

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Glycerol would stimulate the production of poly(gamma-glutamic acid) (gamma-PGA) and decrease its molecular weight in Bacillus subtilis NX-2. When 20 g/l glycerol was added in the medium, the yield of gamma-PGA increased from 26.7 +/- 1.0 to 31.7 +/- 1.3 g/l, and molecular weight of gamma-PGA decreased from 2.43 +/- 0.07 x 10(6) to 1.86 +/- 0.06 x 10(6) Da. In addition, it was found that the decrease of gamma-PGA chain length by glycerol would lead to the decrease of broth viscosity during the fermentation and enhanced the uptake of substrates, which could not only improve cell growth but also stimulate gamma-PGA production. Moreover, it was also found that glycerol could effectively regulate molecular weight between 2.43 +/- 0.07 x 10(6) and 1.42 +/- 0.05 x 10(6) Da with the concentration ranging from 0 to 60 g/l. This was the first time to discover such contribution of glycerol on gamma-PGA production in Bacillus genus. And the effects of glycerol on molecular weight of gamma-PGA would be developed to be an approach for the regulation of microbial gamma-PGA chain length, which is of practical importance for future commercial development of this polymer.

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Biosynthesis of poly (γ-glutamic acid) from l-glutamine, citric acid and ammonium sulfate in Bacillus subtilis IFO3335

Cited by 66 publications

References 21 publications

Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

Enhanced production of poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 in solid state fermentation

Contribution of Glycerol on Production of Poly(γ-Glutamic Acid) in Bacillus subtilis NX-2

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