Biosynthesis of oleanolic acid and its glycosides in Calendula officinalis suspension culture

Szakiel, Anna; Grzelak, Anna; Dudek, Paulina; Janiszowska, Wirginia

doi:10.1016/s0981-9428(03)00018-4

Cited by 18 publications

(13 citation statements)

References 14 publications

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“…The same was shown for the precursors of the TG biosynthesis [ 95 ], elicitors [ 99 , 118 ] and stress hormones [ 117 , 119 ]. Interestingly, the rate of biosynthesis of various triterpenoids by the same cell culture could vary signifi cantly depending on additives to the cultivation medium.…”

Section: Quantitative Compositionsupporting

confidence: 60%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

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Section: Quantitative Compositionsupporting

confidence: 60%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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“…It has been shown that the isoprenoid pathway leading to triterpenoids is operative in all established cultures. Moreover, all oleanolic acid ''glucosides'' and ''glucuronides'', characteristic of leaves of marigold plant, are synthesized after supplying the cultures with [3) 3 H]oleanolic acid (Szakiel et al, 2003a). The biosynthesis of oleanolic acid glycosides as well as their release in the extracellular medium are dependent mainly on light and hormonal conditions of the culture.…”

Section: Calendula Officinalis Suspension Culturementioning

confidence: 99%

Saponins in Calendula officinalis L. – Structure, Biosynthesis, Transport and Biological Activity

2005

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Trends in research on Calendula officinalis L. saponins performed in Department of Plant Biochemistry at Warsaw University are reviewed. Calendula officinalis, a well known medicinal plant, contains significant amounts of oleanane saponins, which form two distinct series of related compounds, called ''glucosides'' and ''glucuronides'' according to the structure of the respective precursor. Both series differ in the pathway of their biosynthesis and further metabolism, i.e. the rate of formation and stages of possible degradation; distribution in the single cell and in the whole plant, including accumulation sites; and the possible physiological role played in the plant according to appropriate biological activities.

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“…Nevertheless, the cultured plant cells, tissues or organs usually display different and sometimes unpredictable profiles of phytochemicals, particularly secondary metabolites, compared with the natural intact plants. In our laboratory, several types of in vitro cultures of the well-known medicinal plant Calendula officinalis L. (common name: marigold; family Asteraceae, tribe Calenduleae) have been successfully induced for the production of saponins (glycosides) of the pentacyclic triterpene oleanolic acid (Szakiel et al, 2003;Wiktorowska et al, 2010;Długosz et al, 2013), but the content of other triterpenoids occurring in non-glycosidic forms, i.e., free and ester forms of steroids and triterpenes, was not elucidated. In addition, it may be worthwhile to obtain some other compounds, such as phytosterols and triterpene alcohols (Muley et al, 2009), from in vitro cultures.…”

Section: Introductionmentioning

confidence: 99%

The content of free and esterified triterpenoids of the native marigold (Calendula officinalis) plant and its modifications in in vitro cultures

Niżyński

Alsoufi

Pączkowski

et al. 2015

Phytochemistry Letters

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Biosynthesis of oleanolic acid and its glycosides in Calendula officinalis suspension culture

Cited by 18 publications

References 14 publications

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Saponins in Calendula officinalis L. – Structure, Biosynthesis, Transport and Biological Activity

The content of free and esterified triterpenoids of the native marigold (Calendula officinalis) plant and its modifications in in vitro cultures

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