Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Shen, Xiao‐Fei; Chu, Feifei; Lam, Paul K.S.; Zeng, Raymond J.

doi:10.1016/j.watres.2015.06.003

Cited by 83 publications

(54 citation statements)

References 37 publications

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“…For example, in autotrophic cultivation, El-Sheekh et al reported that the lipid productivity decreased from 26 mg L À1 d À1 to 1 mg L À1 d À1 after nitrogen starvation because of the inhibition of growth, although the lipid content increased more than fifty percent [26] (Table 5). Moreover, in our previous work, the FAME productivity of Chlorella vulgaris, which was cultivated heterotrophically with glucose, declined from 212 mg L À1 d À1 to 130 mg L À1 d À1 even though the lipid content of dry weight increased to as high as 89% after nitrogen starvation [27] (Table 5). However, in this study, when nitrogen was completely removed and phosphorus was available, biomass productivity declined Fig.…”

Section: The Effect Of Nitrogen Deficiency On Heterotrophic Cultivationmentioning

confidence: 81%

“…It contradicts with most of previous studies concerned with nitrogen deficiency. A comparison of nitrogen effect on biomass productivity, lipid content and lipid productivity with literature, including both autotrophic [15,26,28,29] and heterotrophic cultivation [17,27], is shown in Table 5, Most of the lipid productivities in these studies decreased after nitrogen starvation or limitation. Although Liang et al [30] reported similar biomass productivities between nitrogen sufficient (87 mg L À1 d À1 ) and nitrogen deficiency (79 mg L À1 d À1 ) conditions in a heterotrophic culture with 1% acetate, lipid productivity in nitrogen deficient culture only increased from 27 mg L À1 d À1 to 29 mg L À1 d À1 relative to the control.…”

Section: The Effect Of Nitrogen Deficiency On Heterotrophic Cultivationmentioning

confidence: 96%

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Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation

Shen¹,

Liu

Chu³

et al. 2015

Applied Energy

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Section: The Effect Of Nitrogen Deficiency On Heterotrophic Cultivationmentioning

confidence: 81%

Section: The Effect Of Nitrogen Deficiency On Heterotrophic Cultivationmentioning

confidence: 96%

Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation

Shen¹,

Liu

Chu³

et al. 2015

Applied Energy

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“…; Shen et al . ); but in all cases the productivity of lipids is low. Therefore there is a need for further optimizing productivity and content of lipids in algae.…”

Section: Strategies For Improvement Of Lipid Yieldsmentioning

confidence: 89%

“…Additionally, Shen et al . () obtained in C. vulgaris a lipid content of up to 89% of total dry weight. Differences in lipid productivity between algae could be related to physiological response of each species of algae, under the culture medium analyzed.…”

Section: Strategies For Improvement Of Lipid Yieldsmentioning

confidence: 97%

Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae

Tamayo-Ordóñez

Ayil‐Gutiérrez

Teyer

et al. 2017

Phycological Research

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SUMMARY Biofuels have been shown to be a promising and highly attractive alternative for minimizing the use of fossil fuels, and microalgae have positioned themselves as potential candidates for production of lipids and other substances of commercial interest. We briefly review recent advances made in microalgae culture conditions and genetic manipulation for improving lipid yields for biofuel production – with both approaches showing similar yields of triacylglycerides, indicating that more work is required for improving lipid yield and accumulation in algae. Aiming at gaining knowledge of algae genetic manipulation and exploring future use of this information for modifying the lipid biosynthesis pathway, we investigated whether some characteristics of enzymes involved in lipid biosynthesis could relate to lipid yield and accumulation in algae. We made an in silico analysis of amino acid sequence of enzymatic domains and modeled tertiary structure of three proteins involved in the biosynthesis of lipids in microalgae: acetyl‐CoA carboxylase, Acyl‐CoA: diacylglycerol acyltransferase, and glycerol‐3‐phosphate acyltransferase. Our results suggest that, based on primary amino acid sequences and tertiary structure of proteins shared by certain algae, it is likely that these proteins may relate to lipid yield and accumulation, which makes bioinformatics a powerful tool for in silico study of proteins and for selecting genes involved in lipid biosynthesis that could be useful for heterologous transformation in algae with the long term objective of improving their yield, accumulation, and fatty acid composition by genetic engineering.

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“…In Chlorella vulgaris , nitrogen deficiency promotes phospholipids of membranes (Shen et al. ). Accumulation of saturated fatty acids and triacylglycerols as storage substances has been observed in Porphyridium cruentum and Parietochloris incisa under nitrogen‐deficit conditions, but docosanoic, arachidonic, and eicosapentaenoic acids increased in the presence of ammonium (Khozin‐Golberg et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of global change factors on fatty acids and mycosporine‐like amino acid production in Chroothece richteriana (Rhodophyta)

González-Silvera

Perez

Korbee

et al. 2017

Journal of Phycology

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Under natural conditions, Chroothece richteriana synthesizes a fairly high proportion of fatty acids. However, nothing is known about how environmental changes affect their production, or about the production of protective compounds, when colonies develop under full sunshine with high levels of UV radiation. In this study, wild colonies of C. richteriana were subjected to increasing temperature, conductivity, ammonium concentrations and photosynthetically active radiation (PAR), and UV radiations to assess the potential changes in lipid composition and mycosporine-like amino acids (MAAs) concentration. The PERMANOVA analysis detected no differences for the whole fatty acid profile among treatments, but the percentages of α-linolenic acid and total polyunsaturated fatty acids increased at the lowest assayed temperature. The percentages of linoleic and α-linolenic acids increased with lowering temperature. γ-linolenic and arachidonic acids decreased with increasing conductivity, and a high arachidonic acid concentration was related with increased conductivity. The samples exposed to UVB radiation showed higher percentages of eicosapentaenoic acid and total monounsaturated fatty acids, at the expense of saturated fatty acids. MAAs accumulation increased but not significantly at the lowest conductivity, and also with the highest PAR and UVR exposure, while ammonium and temperature had no effect. The observed changes are probably related with adaptations of both membrane fluidity to low temperature, and metabolism to protect cells against UV radiation damage. The results suggest the potential to change lipid composition and MAAs concentration in response to environmental stressful conditions due to climate change, and highlight the interest of the species in future research about the biotechnological production of both compound types.

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Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Cited by 83 publications

References 37 publications

Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation

Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation

Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae

Effects of global change factors on fatty acids and mycosporine‐like amino acid production in Chroothece richteriana (Rhodophyta)

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