Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation

Shen, Xiao‐Fei; Liu, Jingjing; Chu, Feifei; Zeng, Raymond J.

doi:10.1016/j.apenergy.2015.08.057

Cited by 41 publications

(17 citation statements)

References 38 publications

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“…This can be explained because in treatment 2, in co-culturing, N is expected to be supplied through the nitrogen fixation relation. It is reported for Scenedesmus species, lipid contents of 50-80% measured gravimetrically [28], when using nitrogen and phosphorus deprivation strategies [31]. Thus, the values of fatty acid content, obtained in the present study, are within the literature range.…”

Section: Fatty Acid Content and Profilesupporting

confidence: 83%

“…Scenedesmus sp. growth has been studied for wastewater treatment [29,30], biofuels production [31,32] and food applications [33]. There are some previous publications about the co-culturing of Scenedesmus with different bacteria in wastewater, showing that it increases biomass production due to a higher CO 2 concentration in the media [34], or its suitability to degrade complex pollutants such as crude oil [35].…”

Section: Introductionmentioning

confidence: 99%

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Symbiotic Co-Culture of Scenedesmus sp. and Azospirillum brasilense on N-Deficient Media with Biomass Production for Biofuels

et al. 2019

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The treatment of nitrogen-deficient agriculture wastewater, arising from the vegetable and fruit processing, is a significant problem that limits the efficiency of its biological treatment. This study evaluates the effectiveness of the symbiotic co-culture of Azospirillum brasilense and Scenedesmus sp., under two nitrogen levels (8.23 mg L−1 and 41.17 mg L−1) and mixing systems (aeration and magnetic stirring), aiming to simultaneously use the N-deficient media for their growth while producing biomass for biofuels. Microalgae growth and biomass composition, in terms of protein, carbohydrate and fatty acid contents, were evaluated at the end of the exponential growth phase (15 days after inoculation). Results show that the symbiotic co-culture of microalgae-bacteria can be effectively performed on nitrogen-deficient media and has the potential to enhance microalgae colony size and the fatty acid content of biomass for biofuels. The highest biomass concentration (103 ± 2 mg·L−1) was obtained under aeration, with low nitrogen concentration, in the presence of A. brasilense. In particular, aeration contributed to, on average, a higher fatty acid content (48 ± 7% dry weight (DW)) and higher colony size (164 ± 21 µm2) than mechanical stirring (with 39 ± 2% DW and 134 ± 21 µm2, respectively) because aeration contribute to better mass transfer of gases in the culture. Also, co-culturing contributed in average, to higher colony size (155 ± 21 µm2) than without A. brasilense (143 ± 21 µm2). Moreover, using nitrogen deficient wastewater as the culture media can contribute to decrease nitrogen and energy inputs. Additionally, A. brasilense is approved and already extensively used in agriculture and wastewater treatment, without known environmental or health issues, simplifying the biomass processing for the desired application.

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Section: Fatty Acid Content and Profilesupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Symbiotic Co-Culture of Scenedesmus sp. and Azospirillum brasilense on N-Deficient Media with Biomass Production for Biofuels

et al. 2019

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“…Notably, lipid accumulation in diatoms has been shown to depend on silicate depletion, since this type of microalgae relies on silica for growth . Recent reports with Chlorella vulgaris , Scenedesmus sp and S. obliquus using glucose or acetate as carbon source in batch cultures reassessed the positive effect on lipid accumulation after nitrogen limitation (Table ) . These results also show that cultivation strategies, such as fed‐batch cultures are necessary to obtain high cell density cultures and therefore high lipid productivities.…”

Section: Lipidsmentioning

confidence: 75%

Heterotrophic cultivation of microalgae: production of metabolites of commercial interest

Morales‐Sánchez

Martinez-Rodriguez

Martı́nez

2016

J. Chem. Technol. Biotechnol.

122

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Several microalgal species are capable of growing heterotrophically, exhibiting considerable metabolic versatility and flexibility. As demonstrated in this review, heterotrophic conditions can enhance the biomass concentration by as much as 25-fold compared with phototrophic conditions. Currently, these types of cultivation are economically feasible only for high-value products, including polyunsaturated fatty acids (PUFAs), pigments, antioxidants, polysaccharides, food and aquaculture feed from carbon sources, such as glucose, acetate or glycerol. To make heterotrophic cultivation economically viable for high-volume, low-value commodities, such as biofuels, the use of unconventional carbon sources, such as food and agricultural wastes and wastewater, is recommended. Since microalgae are capable of modifying their metabolism according to varying culture conditions, it is possible to modify, control and therefore maximize the production of target compounds. This manuscript not only offers a review of the most relevant and recent findings in the use of heterotrophic microalgal cultivation for enhanced metabolite production but also provides recommendations for future research on this promising subject.Fatty acids (FAs) are the base structure of almost all membrane lipids, making them an essential biomolecule for the viability of every cell. In all organisms, except for archaea, where the side chains of the membrane lipids are isoprenoids, FAs are organic molecules consisting of an aliphatic carbon chain with a carboxylic J Chem Technol Biotechnol 2017; 92: 925-936 HYDROCARBONSHydrocarbons are composed of a heterogeneous group of molecules of different sizes, shapes and/or lengths and molecular wileyonlinelibrary.com/jctb

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“…An irradiance of 1200 µE/(m 2 · s) was used because it was the maximum for the white SMD LED. Another condition considered was the percentage of N in the medium, Shen et al [18] concluded that a reduction in N did not cause a notable decrease in the microalgal growth rate. However, a lack of N increased the amount of lipids produced compared to lipid production in N-saturated medium.…”

Section: Optimization Of Experimental Conditionsmentioning

confidence: 99%

“…The NR fluorophore (9-diethylamino-5H-benzo[α] phenoxazine-5-one) is a hydrophobic and metachromatic coloring agent that allows the detection and quantification of the microalgal neutral lipid content [17][18][19][20] . Lipid accumulation in the microalgae was periodically established by using this technique.…”

Section: Lipid Vacuoles Determinationmentioning

confidence: 99%

Comparison of lipid extraction methods for the microalgae Acutodesmus obliquus

Hurtado

Garz髇-Castro

Cort閟-Romero

et al. 2018

International Journal of Agricultural and Biological Engineering

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Microalgae are widely used in the pharmaceutical and energy industries, therefore the conditions for their cultivation and extraction methods play an important role in the profiling and acquisition of lipids. The efficiency of lipid extraction from microalgae has attracted great interest from industry because of the wide variety of lipids and amounts that can be obtained. Acutodesmus obliquus (Scenedesmus obliquus UTEX 393) was used in this study. It was cultivated in Bold 3N medium modified with 75% nitrogen at 25°C, pH 6.8, 125 r/min and a photoperiod of 18/6 h and illuminated with white light provided by a Light-Emitting Diode Surface Mount Device extensions (LED SMD) with an intensity of 1200 µE/(m 2 · s). The cells were stained with the Red Nile (RN) technique to indicate lipid production. Four extraction methods were compared, classical, microwave (MW), Soxhlet, and ultrasound (US), using the same solvent proportions (hexane: chloroform: methanol=1 : 2 : 3 ). All samples were analyzed with Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography coupled to Mass Spectrometry (GC-MS). The results showed: 1) lipid production detected by RN was consistent with microalgal growth; 2) the MW technique was the best extraction method, according to the statistical analysis through Randomized Complete Block (RCB) design and performance of 4.6%; and 3) the presence of saturated and unsaturated acids was indicated by FTIR spectra. GC-MS was able to identify palmitic and linoleic acids as the likely major constituents of the sample.

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Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation

Cited by 41 publications

References 38 publications

Symbiotic Co-Culture of Scenedesmus sp. and Azospirillum brasilense on N-Deficient Media with Biomass Production for Biofuels

Symbiotic Co-Culture of Scenedesmus sp. and Azospirillum brasilense on N-Deficient Media with Biomass Production for Biofuels

Heterotrophic cultivation of microalgae: production of metabolites of commercial interest

Comparison of lipid extraction methods for the microalgae Acutodesmus obliquus

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