Biosynthesis of essential fatty acids in Octopus vulgaris (Cuvier, 1797): Molecular cloning, functional characterisation and tissue distribution of a fatty acyl elongase

Monroig, Óscar; Guinot, Diana; Hontoria, Francisco; Tocher, Douglas R.; Navarro, Juan Carlos

doi:10.1016/j.aquaculture.2012.07.016

Cited by 67 publications

(89 citation statements)

References 70 publications

(74 reference statements)

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“…The result is consistent to the previous study in the mollusks [4,27] and in the vertebrates [24,28].…”

Section: Discussionsupporting

confidence: 83%

“…Instead bivalves have been thought to obtain the PUFAs from their diets [30,31]. However, in the present study, the oyster was identified to have the ability to biosynthesize PUFAs by itself, and previous study in two mollusks of O. vulgaris [4] and C. nobilis [16] had the same findings, respectively. Therefore, we conclude that marine mollusks have the ability to biosynthesize PUFAs by themselves.…”

Section: Discussionsupporting

confidence: 56%

“…As the second largest Phylum in animal kingdom, mollusks have provided delicious and health food for human due to their enriched higher protein and PUFAs [23,25,26]. However, mechanism of their enrichment in PUFAs has not been clearly described, although there have been a few reports about biosynthesis PUFAs mechanism in an octopus [4,5] and a bivalve [27] in recent years.…”

Section: Discussionmentioning

confidence: 99%

“…In vertebrates, the pathway of biosynthesis LC-PUFAs has been clearly described [2], which involves sequential desaturation and elongation of essential PUFA precursors, LA and ALA. While in molluscs, fatty acyl elongase genes with differing fatty acid (FA) substrate specificities are only described in Chlamys nobilis and Octopus vulgaris [3,4,5].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Cloning and Functional Characterisation of a Polyunsaturated Fatty Acid Elongase in a Marine Bivalve Crassostrea angulata

Zhang¹,

Liu²,

Cheng³

et al. 2018

JFNR

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The elongases of fatty acids (ELO) are essential for long chain polyunsaturated fatty acid (LC-PUFA) biosynthesis, and their activities depend on the substrates. The full length cDNA of Crassostrea angulata ELO (CaELO) was cloned by RACE PCR and its function was confirmed. The CaELO encodes a polypeptide of 309 amino acid residues, which containes a histidine box HXXHH motif conserved in all elongases and shares high similarity to the elongases of Chlamys nobilis and Octopus vulgaris. Phylogenetic analysis showed that the putative elongase was placed in the same group with ELOVL2 and ELOVL5, which have been demonstrated to be critical enzymes participating in the biosynthesis of PUFAs in vertebrates. When expressed in Saccharomyces cerevisiae, CaELO was able to elongate n-3 and n-6 PUFA substrates with chain lengths of C18 and C20, indicating that the CaELO had similar substrate specificities to vertebrate ELOVL5. CaELO had lower activity to elongate monounsaturated fatty acids, but had not activity to saturated fatty acids. Interestingly, the conversion rate of PUFAs depended on the length of carbon chain, the number of double bond, and n-3 / n-6 series in the species.

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“…The result is consistent to the previous study in the mollusks [4,27] and in the vertebrates [24,28].…”

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Cloning and Functional Characterisation of a Polyunsaturated Fatty Acid Elongase in a Marine Bivalve Crassostrea angulata

Zhang¹,

Liu²,

Cheng³

et al. 2018

JFNR

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“…Table 1. Bioconversion ability of 18:3-n3 (Kanazawa et al, 1979) In recent years, numerous studies on the abilities of fatty acid bioconversion in freshwater and marine fish have been carried out (Monroig et al, 2012Morais et al, 2012;Fonseca-Madrigal et al, 2014). It has been demonstrated that a freshwater fish may have a marine pattern of fatty acid elongation and desaturation or vice versa.…”

Section: Essential Fatty Acids Of Marine and Freshwater Fishmentioning

confidence: 99%

Roles of lipids and fatty acids through the spermatogenesis of European eel (Anguilla anguilla)

Ariño¹

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AgradecimientosDesde bien pequeña he tenido miedo al mar. Con el tiempo descubrí que no era miedo lo que sentía sino curiosidad. Era todo un misterio para mí lo que ocultaba el océano y esa intriga a lo desconocido, es lo que de pequeña confundía con miedo. No todo el mundo es capaz de enfrentarse a sus temores y yo me siento muy afortunada de haber tenido la oportunidad de que aquello que tanta expectación me ha causado siempre, se haya convertido en el área de estudio de mi tesis doctoral. Algunas personas han sido partícipes de que lo consiga y otras, han formado parte de estos años que para mí han sido toda una aventura. Todas ellas han colaborado para que señale esta etapa de mi vida como "inolvidable" y es por eso que siento la necesidad de agradecérselo.En primer lugar me gustaría dar las gracias a mi director de tesis, Johnny. Muchas gracias por apostar por mí todo este tiempo (aunque siempre hayamos tenido nuestras diferencias con el formato de los pósters…jeje). Siempre he tenido muy en cuenta tus consejos, te admiro y para mi has sido un referente en este período, gracias por todo Johnny. También quiero hacer mención a Luz. El trato tan afable que ha tenido siempre hacia mí, me ha hecho sentirla muy cercana y eso se agradece, muchas gracias Luz. Gracias también a los colegas daneses por colaborar con nosotros y, en especial a Ian Butts, por ofrecer su coautoría en el último capítulo.Gracias a los compañeros del grupo. Ila gracias por ser tan servicial conmigo siempre. David, gràcies per preguntarme i preocuparte sempre per la meua tesi i oferir-me la teua ajuda quan ho he necessitat. Morini, que grande el día que llegaste y te lleve "alcampo"…jaja! Gracias por los buenos momentos que hemos pasado y sabes que te debo un concierto de los Mojinos!!!... con una botella de Jägermeister quizás?.... Gracias a Pepa que aunque no hayamos coincidido mucho tiempo, me encanta su forma de ser y siempre me ha animado con la tesis, gracias Pepa! Gracias también a los compañeros de laboratorio y en especial a Javi Moya.No puedo dejar de lado mi paso por nutrición. Silvia y Ana, os estoy profundamente agradecida porque vosotras fuisteis las primeras personas que me dieron una oportunidad en el mundo de la investigación, nunca lo he olvidado y ahora que tengo la oportunidad os digo en mayúsculas: GRACIAS! Mi paso por nutrición fue breve pero me llevo muy buenos recuerdos: el hundimiento del Enterprise, Javi Todopoderoso, Nacho, Nuri, Jorge, Miguel, Andrés… y el mejor recuerdo: Silvia Nogales. Silvi, para mi has sido un poco como el señor Miyagi… estar contigo ha sido un continuo aprendizaje, aunque a veces ni me diera cuenta (…dar cera, pulir cera…jeje).Gracias a las personas más importantes en mi vida, mi familia. Gràcies mamà pel teu suport incondicional. Papa, aunque a día de hoy no tengas muy claro lo que hago con las anguilas, nunca me has cuestionado y siempre te ha bastado con verme feliz, gracias. Gracias a mi hermano y Beti que, aunque por desgracia están a miles de Km siempre me han transmitido su apoyo. Y gr...

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Insights into the phylogenetic and molecular evolutionary histories of Fad and Elovl gene families in Actiniaria

Surm

Toledo

Prentis

et al. 2018

Ecology and Evolution

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The biosynthesis of long‐chain polyunsaturated fatty acids (LC‐PUFAs, ≥ C20) is reliant on the action of desaturase and elongase enzymes, which are encoded by the fatty acyl desaturase (Fad) and elongation of very long‐chain fatty acid (Elovl) gene families, respectively. In Metazoa, research investigating the distribution and evolution of these gene families has been restricted largely to Bilateria. Here, we provide insights into the phylogenetic and molecular evolutionary histories of the Fad and Elovl gene families in Cnidaria, the sister phylum to Bilateria. Four model cnidarian genomes and six actiniarian transcriptomes were interrogated. Analysis of the fatty acid composition of a candidate cnidarian species, Actinia tenebrosa, was performed to determine the baseline profile of this species. Phylogenetic analysis revealed lineage‐specific gene duplication in actiniarians for both the Fad and Elovl gene families. Two distinct cnidarian Fad clades clustered with functionally characterized Δ5 and Δ6 proteins from fungal and plant species, respectively. Alternatively, only a single cnidarian Elovl clade clustered with functionally characterized Elovl proteins (Elovl4), while two additional clades were identified, one actiniarian‐specific (Novel ElovlA) and the another cnidarian‐specific (Novel ElovlB). In actiniarians, selection analyses revealed pervasive purifying selection acting on both gene families. However, codons in the Elovl gene family show patterns of nucleotide variation consistent with the action of episodic diversifying selection following gene duplication events. Significantly, these codons may encode amino acid residues that are functionally important for Elovl proteins to target and elongate different precursor fatty acids. In A. tenebrosa, the fatty acid analysis revealed an absence of LC‐PUFAs > C20 molecules and implies that the Elovl enzymes are not actively contributing to the elongation of these LC‐PUFAs. Overall, this study has revealed that actiniarians possess Fad and Elovl genes required for the biosynthesis of some LC‐PUFAs, and that these genes appear to be distinct from bilaterians.

show abstract

Biosynthesis of essential fatty acids in Octopus vulgaris (Cuvier, 1797): Molecular cloning, functional characterisation and tissue distribution of a fatty acyl elongase

Cited by 67 publications

References 70 publications

Molecular Cloning and Functional Characterisation of a Polyunsaturated Fatty Acid Elongase in a Marine Bivalve <i>Crassostrea</i><i> </i><i>angulata</i>

Molecular Cloning and Functional Characterisation of a Polyunsaturated Fatty Acid Elongase in a Marine Bivalve <i>Crassostrea</i><i> </i><i>angulata</i>

Roles of lipids and fatty acids through the spermatogenesis of European eel (Anguilla anguilla)

Insights into the phylogenetic and molecular evolutionary histories of Fad and Elovl gene families in Actiniaria

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