Biosynthesis of chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach

Embaby, Amira M.; Melika, Ramy R.; Hussein, Ahmed; El-Kamel, Amal H.; Marey, H. S.

doi:10.1016/j.procbio.2017.09.030

Cited by 16 publications

(5 citation statements)

References 61 publications

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“…The end products of pullulan, dextrin, and starch hydrolysis were tested by thin layer chromatography (TLC). Briefly silica gel plates [0.2 mm silica gel-coated aluminum plates (Kiesel gel 60 F254; Merck, NJ)] using a solvent mixture of n- propanol: ammonia: d. H 2 O with a volume ratio 7:1:2 (v:v:v) (Embaby et al 2018 ). The end products of pullulan, starch, and dextrin hydrolysis (10 µL) each were loaded in the silica gel plates.…”

Section: Methodsmentioning

confidence: 99%

A molecular study on recombinant pullulanase type I from Metabacillus indicus

et al. 2023

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Despite the great potential of cold-adapted pullulanase type I in tremendous industrial applications, the majority of commercialized pullulnases type I are of mesophilic and thermophilic origin so far. Hence, the present study underlines cloning, heterologous expression in Escherichia coli, characterization, and in silico structural modeling of Metabacillus indicus open reading frame of cold-adapted pullulanase type I (Pull_Met: 2133 bp & 710 a.a) for the first time ever. The predicted Pull_Met tertiary structure by I-TASSER, was structurally similar to PDB 2E9B pullulanase of Bacillus subtilis. Purified to homogeneity Pull_Met showed specific activity (667.6 U/mg), fold purification (31.7), molecular mass (79.1 kDa), monomeric subunit and Km (2.63 mg/mL) on pullulan. Pull_Met had optimal pH (6.0) and temperature (40 oC). After 10 h pre-incubation at pH 2.6-6.0, Pull_Met maintained 47.12 ± 0.0–35.28 ± 1.64% of its activity. After 120 min pre-incubation at 30 oC, the retained activity was 51.11 ± 0.29%. At 10 mM Mn2+, Na2+, Ca2+, Mg2+, and Cu2+ after 30 min preincubation, retained activity was 155.89 ± 8.97, 134.71 ± 1.82, 97.64 ± 7.06, 92.25 ± 4.18, and 71.28 ± 1.10%, respectively. After 30 min pre-incubation with Tween-80, Tween-20, Triton X-100, and commercially laundry detergents at 0.1% (v/v), the retained activity was 141.15 ± 3.50, 145.45 ± 0.20, 118.12 ± 11.00, and 90%, respectively. Maltotriose was the only end product of pullulan hydrolysis. Synergistic action of CA-AM21 (α-amylase) and Pull_Met on starch liberated 16.51 g reducing sugars /g starch after 1 h at 40 oC. Present data (cold-adeptness, detergent stability, and ability to exhibit starch saccharification of Pull_Met) underpins it as a promising pullulanase type I for industrial exploitation.

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Section: Methodsmentioning

confidence: 99%

A molecular study on recombinant pullulanase type I from Metabacillus indicus

et al. 2023

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“…As a consequence, the shortages included in the OFAT approach were covered by employing the statistical sequential PBD and RSM designs. The statistical, sequential, and experimental designs are being broadly employed for the optimization of fermentation factors in the production of enzymes [ 23 , 48 ], antibiotics biodegradation [ 49 ], production of microbial pigments [ 50 ], production of extracellular pecticoligosaccharides [ 51 ], biosynthesis of chitooligosaccharides [ 52 ], biodegradation of chicken feather [ 53 ], etc. RSM covers a small number of experimental trials with a large number of factors.…”

Section: Discussionmentioning

confidence: 99%

Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774

Matrawy

Khalil

Marey

et al. 2021

JoF

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The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel Penicillium chrysogenum strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett–Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 °C), WS, and ammonium sulphate were the key determinants prompting xylanase production; inferred from OFAT. The WS concentration (%(w/v)), yeast extract concentration (%(w/v)), and initial pH of the production medium imposed significant effects (p ≤ 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (w/v), 5.1, and 0.098% (w/v), respectively; 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase.

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“…purpureus strain ATCC16436 was subjected to a detailed three step statistical modeling approach; OVAT, PBD, and CCD. Statistical modeling is a robust approach towards optimization of the yield of outputs from various chemical and biological processes [33–35]. In this regard, the optimal level of each key determinant controlling the monascus pigments production from the candidate fungal strain was localized exactly to avoid the usage of extra amounts of medium components.…”

Section: Discussionmentioning

confidence: 99%

Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach

Embaby¹,

Hussein²,

Hussein³

2018

PLoS ONE

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The present study underlines a statistically optimized, low cost, effective approach for efficient co-valorization of two non-efficiently utilized, highly accumulated, raw agro-industrial wastes: corn cob and glycerol for co-production of natural biopigments: monascus orange and red pigments by the aid of Monascus purpureus strain ATCC 16436. A three step sequential, statistical modeling approach: one variable at a time (OVAT), Plackett-Burman design (PBD), and central composite design (CCD) was employed to optimize the production of monascus pigments using co-solid state fermentation of the two raw agro-industrial wastes. Corn cob among other carbon sources (e.g., rice grains, sugarcane bagasse, and potato peel) was the most appropriate substrate triggering co-production of orange and red monascus pigments; deduced from OVAT. Glycerol and inoculum size proved to impose significant consequences (P<0.05) on the production of monascus pigments as inferred from PBD. The optimal levels of inoculum size (12 x 1011 spores/mL) and glycerol (2.17 M) did achieve a maximal color value of 133.77 and 108.02 color value units/mL of orange and red pigments, respectively at 30 oC after 10 days; concluded from CCD with an agitation speed of 150 rpm. Present data would underpin the large scale production of monascus pigments using the present approach for efficient exploitation of such biopigments in food, pharmaceutical and textile industries.

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Biosynthesis of chitosan-Oligosaccharides (COS) by non-aflatoxigenic Aspergillus sp. strain EGY1 DSM 101520: A robust biotechnological approach

Cited by 16 publications

References 61 publications

A molecular study on recombinant pullulanase type I from Metabacillus indicus

A molecular study on recombinant pullulanase type I from Metabacillus indicus

Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774

Monascus orange and red pigments production by Monascus purpureus ATCC16436 through co-solid state fermentation of corn cob and glycerol: An eco-friendly environmental low cost approach

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