Biosynthesis and stabilization of Au and Au–Ag alloy nanoparticles by fungus,<i>Fusarium semitectum</i>

Sawle, Balaji D.; Salimath, Basavaraja; Deshpande, Raghunandan; Bedre, Mahesh D.; Prabhakar, B.; Venkataraman, A.

doi:10.1088/1468-6996/9/3/035012

Cited by 108 publications

(14 citation statements)

References 24 publications

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“…The dielectric, magnetic, optical, antimicrobial, imaging, and catalytic properties of copper make this element a candidate for applications in photoelectrochemical cells, sensors, solar cells, inks, and antimicrobial coatings ( 1 , 2 ). Recent studies have also found that many microorganisms, such as bacteria ( 3 , 4 ) and fungi ( 5 ), can produce inorganic nanoparticles (NPs), such as Ag ( 6 ), Au ( 7 ), Cu ( 8 , 9 ), CuO ( 10 , 11 ), and magnetite ( 12 ), among others ( 13 ). A few studies described the synthesis of Cu NPs ( 8 , 9 ) ranging from 10 to 40 nm intra- and extracellularly by a sustainable method using bacteria.…”

Section: Introductionmentioning

confidence: 99%

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

et al. 2021

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The chemical synthesis of monoatomic metallic copper is unfavorable and requires inert or reductive conditions and the use of toxic reagents. Here, we report the environmental extraction and conversion of CuSO4 ions into single-atom zero-valent copper (Cu0) by a copper-resistant bacterium isolated from a copper mine in Brazil. Furthermore, the biosynthetic mechanism of Cu0 production is proposed via proteomics analysis. This microbial conversion is carried out naturally under aerobic conditions eliminating toxic solvents. One of the most advanced commercially available transmission electron microscopy systems on the market (NeoArm) was used to demonstrate the abundant intracellular synthesis of single-atom zero-valent copper by this bacterium. This finding shows that microbes in acid mine drainages can naturally extract metal ions, such as copper, and transform them into a valuable commodity.

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Section: Introductionmentioning

confidence: 99%

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

et al. 2021

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“…The green synthesis of nanoparticles has attracted many researchers because of the high demand to produce clean, nontoxic chemicals, environmentally benign solvents, and renewable materials. Moreover, this methodology improves the biocompatibility of the obtained NPs [ 9 , 10 , 11 ]. For instance, many successful attempts have been reported in the literature, which achieve the synthesis of nanoparticles using biological systems, such as yeast, fungi, and bacteria [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Successful Green Synthesis of Gold Nanoparticles using a Corchorus olitorius Extract and Their Antiproliferative Effect in Cancer Cells

Ismail

Saqer

Assirey

et al. 2018

IJMS

102

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A facile bottom-up “green” synthetic route of gold nanoparticles (Au NPs) is described, using a leaf extract of the Malvaceae plant Corchorus olitorius as a reducing and stabilizing agent. The size and shape of the obtained nanoparticles were modulated by varying the amounts of the metal salt and the broth extract in the reaction medium. Only one hour was required for the complete conversion to Au NPs, suggesting that the reaction rate was higher or comparable to those of nanoparticles synthesized by chemical methods. The obtained nanoparticles were characterized by UV–visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and thermal gravimetric analysis (TGA). While infrared spectroscopy was employed to characterize the various functional groups in the organic layer that stabilized the particles, TEM images were used to optimize the conditions for NPs growth. A low concentration of the C. olitorius extract yielded mixed triangular and hexagonal shapes; in contrast, quasi-spherical shapes of Au NPs with an average size of 37–50 nm were obtained at a higher extract broth concentration. The Au NPs displayed Surface Plasmon Resonance (SPR) bands at 535 nm. An in vitro cytotoxic assay of the biocompatible Au NPs revealed a strong cytotoxic activity in three human cancer cell lines, namely, colon carcinoma HCT-116, hepatocellular carcinoma HepG-2, and breast adenocarcinoma MCF-7. In-silico bioactivity, drug-likeness, and ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) predictions were conducted in order to examine the pharmacokinetic behavior of the compounds present in the C. olitorius extract.

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“…Bioreduction of gold ions by biomolecules in plant leaf extracts are the likely reason for this observation [33,34]. FT-IR spectra with an analysis of vibrations is a suitable means for measuring minor structures in interactions between metallic nanoparticles and biomolecules [35]. Figure 4 showed that the N–H bonding vibration of leading amines and C–N elongation and coincidence of aliphatic amines have the robust capability to bind metal; hence, the phytochemicals from the plant extract putatively make a coating that shields the metal nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of Gold Nanoparticles (AuNPs) Using Ricinus communis Leaf Ethanol Extract, Their Characterization, and Biological Applications

et al. 2019

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The purpose of this study was to explore the collective biological properties of Ricinus communis ethanol leaf extract (RcExt) and extract-fabricated gold nanoparticles (RcExt-AuNPs). AuNPs were synthesized using RcExt. Fingerprint data of the biochemicals putatively found in RcExt were obtained using gas chromatography–mass spectrometry (GC-MS/MS) and high-performance liquid chromatography/ultraviolet-visible (HPLC/UV-VIS) analyses. RcExt-AuNPs were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), and Fourier- transform infrared radiation (FTIR) spectroscopy. Cytotoxic activity on the Hela and HepG2 tumor cell lines was tested through cell viability, antimicrobial activity against bacterial and fungal pathogens through a well diffusion assay, hemolytic activity on red blood cells through absorbance reading, and stimulatory/inhibitory effects on splenic cells by cell viability. AuNPs of 200 nm size were synthesized. GC-MS/MS analysis revealed 12 peaks and HPLC/UV-VIS analysis resulted in 18, 13, and five peaks at the wavelengths of 220, 254, and 300 nm, respectively. Cytotoxicity screening revealed that RcExt had stimulatory effects (6.08%) on Hela cells and an inhibitory effect (−28.33%) on HepG2 cells, whereas RcExt-AuNPs showed inhibitory effects (−58.64% and −42.74%) on Hela and HepG2 cells, respectively. Antimicrobial activity of RcExt-AuNPs against tested pathogens was significantly higher (average diameters of inhibition zones were higher (ranging from 9.33 mm to 16.33 mm)) than those of RcExt (ranging from 6.00 mm to 7.33 mm). RcExt and RcExt-AuNPs showed 4.15% and 100% lytic effects, respectively. Inhibitory effects on splenic cells for RcExt-AuNPs were observed to be significantly higher (−30.56% to −72.62%) than those of RcExt (−41.55% to −62.25%) between concentrations of 25 to 200 µg/mL. RcExt-AuNPs were inhibitory against HepG2 and Hela cells, while RcExt inhibited HepG2 but stimulated Hela cells. RcExt-AuNPs showed comparatively more antimicrobial activity. RcExt was safe while RcExt-AuNPs harmful to red blood cells (RBCs). RcExt and RcExt-AuNPs showed inhibitory effects on splenic cells irrespective of dose.

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Biosynthesis and stabilization of Au and Au–Ag alloy nanoparticles by fungus,Fusarium semitectum

Cited by 108 publications

References 24 publications

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper

Successful Green Synthesis of Gold Nanoparticles using a Corchorus olitorius Extract and Their Antiproliferative Effect in Cancer Cells

Synthesis of Gold Nanoparticles (AuNPs) Using Ricinus communis Leaf Ethanol Extract, Their Characterization, and Biological Applications

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