Biosynthesis and Function of Eicosanoids in the Uterus

Chaudhuri, Gautam

doi:10.1007/978-1-4613-0575-0_14

Cited by 5 publications

(3 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these studies, we cannot rule out the possibility that during the 18 h following breeding and before muscle was harvested, release of endometrial substances, such as nitric oxide [33,34], prostaglandins [35], and other inflammatory cytokines [36], caused changes within the muscle cell that persisted in the absence of the endometrium. Changes such as suppression of myometrial gap junction numbers or function [37], altered phosphorylation state of contractile proteins, or expression of muscle cell enzymes integral to contractile function [35] may have contributed to the reduced contractile response of the myometrium isolated from OS mares. Because myometrial function was not evaluated before insemination, we cannot evaluate the role of endometrial uterotonins in the contractile defect of myometrium from OS mares in the present study.…”

Section: Discussionmentioning

confidence: 95%

Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function1

Rigby

Barhoumi

Burghardt

et al. 2001

Biology of Reproduction

View full text Add to dashboard Cite

Persistent, postmating endometritis affects approximately 15% of mares and results in reduced fertility and sizable economic losses to the horse-breeding industry. Mares that are susceptible to postmating endometritis have delayed uterine clearance associated with reduced uterine contractility. Unfortunately, the mechanism for reduced uterine contractility remains an enigma. The present study examined the hypothesis that mares with delayed uterine clearance have an intrinsic contractile defect of the myometrium. Myometrial contractility was evaluated in vitro by measuring isometric tension generated by longitudinal and circular uterine muscle strips in response to KCl, oxytocin, and prostaglandin F(2alpha) (PGF(2alpha)) for young nulliparous mares, older reproductively normal mares, and older mares with delayed uterine clearance. In addition, intracellular Ca(2+) regulation was evaluated using laser cytometry to measure oxytocin-stimulated intracellular Ca(2+) transients of myometrial cells loaded with a Ca(2+)-sensitive fluorescent dye, fluo-4. For all contractile agonists, myometrium from mares with delayed uterine clearance failed to generate as much tension as myometrium from older normal mares. Oxytocin-stimulated intracellular Ca(2+) transients were similar for myometrial cells from mares with delayed uterine clearance and from older normal mares, suggesting that the contractile defect did not result from altered regulation of intracellular Ca(2+) concentration. Furthermore, no apparent age-dependent decline was observed in myometrial contractility; KCl-depolarized and oxytocin-stimulated longitudinal myometrium from young normal mares and older normal mares generated similar responses. However, circular myometrium from young normal mares failed to generate as much tension as myometrium from older normal mares when stimulated with oxytocin or PGF(2alpha), suggesting possible age-related alterations in receptor-second messenger signaling mechanisms downstream of intracellular Ca(2+) release. In summary, for mares with delayed uterine clearance, an intrinsic contractile defect of the myometrium may contribute to reduced uterine contractility following breeding.

show abstract

Section: Discussionmentioning

confidence: 95%

Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function1

Rigby

Barhoumi

Burghardt

et al. 2001

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…Prostaglandins are produced by epithelial and stromal endometrial cells in human and other species (Alecozay, Harper, Scheken & Hanahan, 1991;Zhang, Paria & Davis, 1991;Chen, Yang, Hilsenrath, Le & Harper, 1995). They act in both an endocrine and paracrine manner to increase uterine blood flow, vascular permeability and to regulate menstruation and implantation (Ford, Reynolds & Magness, 1982;Keys, King & Kennedy, 1986;Chaudhuri, 1991;Yee & Kennedy, 1993). Prostaglandins, specifically PGEµ, have been shown to regulate electrolyte transport function in rabbit cortical collecting duct (Sakairi & Jacobson, 1995), prairie dog gallbladder (Saunders-Kirkwood, Cates & Roslyn, 1993) and porcine distal colon epithelium (Traynor, Brown & O'Grady, 1993).…”

mentioning

confidence: 99%

Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E₂

Deachapunya

O’Grady

1998

The Journal of Physiology

View full text Add to dashboard Cite

The objective of this study was to investigate the mechanism of PGE2 regulation of Cl− transport across glandular endometrial cells grown in primary culture. Most of the basal short circuit current (Isc) was inhibited by luminal addition of 5‐nitro‐2‐(3‐phenylpropylamino)benzoic acid (NPPB) or glibenclamide, suggesting the presence of a basally active Cl− conductance in the apical membrane. Basolateral addition of 10 μM PGE2 increased Isc by 41 ± 3 μA. A similar response was observed when cells were treated with 8‐(4‐chlorophenylthio) adenosine 3′,5′‐cyclic monophosphate (CPT‐cAMP). Pretreatment of monolayers with NPPB and glibenclamide blocked the PGE2 and cAMP‐mediated increase in Isc, suggesting that the effects of PGE2 and cAMP were dependent on the activity of an apical NPPB‐ and glibenclamide‐sensitive conductance. Addition of 50 nM antiPGE2 antibody to the basolateral bathing solution decreased basal Isc by 20 % and shifted the threshold response to exogenous PGE2. This result suggests autocrine regulation of electrogenic Cl− transport by PGE2. Experiments with amphotericin B‐permeabilized monolayers revealed that the apical PGE2‐activated, NPPB‐ and glibenclamide‐sensitive conductance was Cl− dependent and that the current‐voltage relationship and anion permeation properties (SCN−>Br− > Cl− > I−) were characteristic of the cystic fibrosis transmembrane conductance regulator (CFTR). Cultured porcine endometrial epithelial cells were specifically labelled with an antibody to a peptide sequence within the regulatory domain of CFTR. The effect of PGE2 was blocked by basolateral addition of bumetanide and furosemide at concentrations that are selective for inhibition of Na+‐K+‐2Cl−cotransport activity. The effect of bumetanide on Isc was Cl− dependent, suggesting a role for the bumetanide‐sensitive transport pathway in Cl− secretion. PGE2 and cAMP also activated an outwardly rectifying basolateral K+ channel which presumably sustains the driving force for electrogenic Cl− efflux across the apical membrane. The concentration‐conductance and concentration‐Isc response relationships for PGE2 showed that basolateral K+ permeability was rate limiting with respect to transepithelial anion secretion and that activation of a basolateral K+ channel by PGE2 was necessary to achieve maximum rates of Cl− secretion.

show abstract

“…These prostaglandins are considered to have an important role in the initiation and/or progression of labour. Nevertheless, the mechanisms underlying their effects of contractile activity and their interactions with other biologic contractile agents are not yet clarified (Chaudhuri 1990).…”

mentioning

confidence: 99%

Distribution of mast cells and the effect of their mediators on contractility in human myometrium

Rudolph

Reinicke

Cruz

et al. 1993

BJOG

View full text Add to dashboard Cite

Objective To examine the distribution of mast cells in human uterine tissue and to study the interactions between mast cell mediators (histamine and serotonin) and PGF,, in human myometrium contractile activity.Design Distribution of mast cells were analysed in cryostat sections of myometrium samples stained with Toluidine blue. Contractile activity was evaluated in an isolated organ bath preparation on myometrial strips obtained from women whose pregnancies ended in elective caesarean section.Setting Biological Science Laboratories at the University of Concepci6n.Subjects Twenty women undergoing elective caesarean and 10 women undergoing hysterectomy at the G. Grant Hospital.Main outcome measurements Cumulative concentration-response curves for histamine and serotonin before and after the addition of subumbral concentrations of the PGF,, or serotonin were performed.

show abstract

Biosynthesis and Function of Eicosanoids in the Uterus

Cited by 5 publications

References 136 publications

Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function1

Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function1

Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E₂

Distribution of mast cells and the effect of their mediators on contractility in human myometrium

Contact Info

Product

Resources

About

Biosynthesis and Function of Eicosanoids in the Uterus

Cited by 5 publications

References 136 publications

Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function1

Mares with Delayed Uterine Clearance Have an Intrinsic Defect in Myometrial Function1

Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E2

Distribution of mast cells and the effect of their mediators on contractility in human myometrium

Contact Info

Product

Resources

About

Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E₂