Distribution of mast cells and the effect of their mediators on contractility in human myometrium

Rudolph, M I; Reinicke, Karin; Cruz, Maria Antonieta; Gallardo, Victoria; González, Claudio; Bardisa, L.

doi:10.1111/j.1471-0528.1993.tb15178.x

Cited by 81 publications

(53 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many authors have described clusters of MCs in the myometrium and endometrium of non-pregnant women, proposing active roles for these cells in the control of implantation and in extracellular matrix remodelling during the menstrual cycle (Mori et al 1997, Vincent et al 2000. Moreover, it has been suggested that MCs could regulate contractility of the myometrium during labour in women (Rudolph et al 1993). A recent study showed that MCs and their mediators are capable of regulating cervical contractility in animals in mid-pregnancy, and possibly contribute to cervical competence during pregnancy in guinea pigs (Bytautiene et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Varayoud¹,

Ramos²,

Bosquiazzo³

et al. 2004

Reproduction

View full text Add to dashboard Cite

During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and a-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.

show abstract

Section: Introductionmentioning

confidence: 99%

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Varayoud¹,

Ramos²,

Bosquiazzo³

et al. 2004

Reproduction

View full text Add to dashboard Cite

show abstract

“…Higher than physiological levels of histamine were required to characterize the histamine receptors involved in SW756 migration. This may be explained by the fact that in physiological conditions mast cells release a plethora of mediators that potentiate and overlap each other, which is not the case when the sole effect of histamine was being analyzed (55,56). The results showed that histamine, acting on H1R and H4R, modulates the migration rate of SW756 cells in a concentration dependent manner.…”

Section: Discussionmentioning

confidence: 67%

The Influence of Mast Cell Mediators on Migration of SW756 Cervical Carcinoma Cells

et al. 2008

Self Cite

View full text Add to dashboard Cite

Abstract. The role of mast cell mediators on cervical cancer cell migration was assessed using an in vitro assay of scratch wound healing onto monolayers of HPV18-positive cervical carcinoma cells (SW756). Migration of SW756 cells was accelerated by co-culture with the mast cell line LAD2. This effect was inhibited by the H1R antagonist pyrilamine and the cannabinoid agonists 2-arachidonylglycerol (2AG) and Win 55,212-2. Therefore, the specific effects of histamine and cannabinoids on SW756 migration and LAD2 activation were analyzed. Histamine added to the in vitro assay of scratch wound healing either increased or inhibited SW756 migration rate by acting either on H1R or H4R, respectively. Cannabinoids acted on CB1 receptors to inhibit SW756 migration. Supernatants from SW756 cells stimulated LAD2 cell degranulation, which in turn was inhibited by cannabinoids acting via CB2 receptors. RT-PCR showed that SW756 expressed mRNA for CB1, CB2, H1R, H2R, and H4R. On the other hand, LAD2 expressed mRNA for all four HRs and CB2. The results suggest that mast cells could be contributing to cervical cancer cell invasion and spreading by the release of histamine and cannabinoids. Therefore, therapeutic modulation of specific mast cell mediators may be beneficial for cervical cancer treatment.

show abstract

“…Spontaneous preterm labor is known to have many of the characteristics of an inflammatory response (Lindström and Bennett, 2005). Given that the uterus has many mast cells in close proximity to the myometrium (Rudolph et al, 1993), it is possible that a drug that inhibited the response of the myometrium to serotonin may have a tocolytic effect that could be used to treat preterm labor. Conversely, 5-HT 2A agonists may have a role in contracting human myometrium, for example, in the induction of labor or in the treatment of atonic postpartum hemorrhage.…”

Section: Discussionmentioning

confidence: 99%

“…Serotonin is also known to modulate contractions of the uterus, and the release of serotonin, after the activation of mast cells or platelets, may be an important source of the ligand in this tissue (Rudolph et al, 1993). The exact effect of serotonin, in terms of inhibiting or stimulating contractions, appears to be species specific.…”

mentioning

confidence: 99%

Characterization of Serotonin Receptors in Pregnant Human Myometrium

Cordeaux

Pasupathy

Bacon³

et al. 2008

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The monoamine, 5-hydroxytryptamine (5-HT), stimulates contraction of human uterine smooth muscle (myometrium), but the receptor subtypes involved have not been characterized. We studied the effects of a range of 5-HT receptor subtype-selective agonists and antagonists in isolated strips of myometrium obtained at the time of caesarean section. The 5-HT 1A receptor agonist, 8-hydroxy-2-dipropylaminotetralin, produced an increase in contractions that was highly variable, of low potency, and was not significantly inhibited by the 5- These cell surface receptors are classified into seven distinct subtypes (5-HT 1 to 5-HT 7 ), and, with the exception of the ligand-gated ion channel 5-HT 3 , they are all members of the G-protein-coupled receptor superfamily (Alexander et al., 2008). In the central nervous system, these receptors are implicated in numerous conditions, including: depression, anxiety, schizophrenia, and migraine (Barnes and Sharp, 1999). Serotonin also plays a role in platelet activation (Kau-

show abstract

Distribution of mast cells and the effect of their mediators on contractility in human myometrium

Cited by 81 publications

References 16 publications

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

The Influence of Mast Cell Mediators on Migration of SW756 Cervical Carcinoma Cells

Characterization of Serotonin Receptors in Pregnant Human Myometrium

Contact Info

Product

Resources

About