Biosynthesis and Compositional Regulation of Poly[(3‐hydroxybutyrate)‐co‐(3‐hydroxyhexanoate)] in Recombinant Ralstonia eutropha Expressing Mutated Polyhydroxyalkanoate Synthase Genes

Abstract

A new strategy for bacterial polyhydroxyalkanoate (PHA) production by recombinant Ralstonia eutropha PHB(-)4 harboring mutated PHA synthase genes (phaC(Ac)) from Aeromona caviae was investigated. The strain harboring wild-type phaC(Ac) gene produced a PHA copolymer consisting of (R)-3-hydroxybutyrate and (R)-3-hydroxyhexanoate [P(3HB-co-3HHx)] with 3.5 mol-% of 3HHx fraction from soybean oil. When the mutants of phaC(Ac) gene were applied to this production system, 3HHx fraction in copolymers was varied in the range of 0-5.1 mol-%. Thus, the regulation of PHA copolymer compositions has been achieved by the use of mutated PHA synthase genes.

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

“…61-3 [39,[52][53][54]. Comparison of the amino sequences of PhaC1 16 protein and other Class I and Class II PHA synthases revealed both low sequence identity and homology with the Class I PHA synthase of R. eutropha H16 (38% identity, 54% homology) and with the Class II PHA synthase of P. putida LS46 (46% identity, 61% homology).…”

Microbial synthesis and enzymatic degradation of renewable poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate]

“…Recombinant plasmids for functional expression of wild-type phaC Ac gene or its mutants in R. eutropha PHB 2 4, pBBREE32d13 and pJRDEE32d13, were constructed using the broad-host-range plasmids pBBR1MCS-2 and pJRD215, respectively [4,6]. These plasmids contain phaC Ac gene with its native promoter region.…”

Isolamento de bactéria produtoras de polihidroxialcanoatos de cadeia curta e média a partir de óleos vegetais

“…Além disso, foram realizados trabalhos de evolução dirigida dos genes da PHA sintase (KICHISE et al, 2002;TSUGE et al, 2004;TSUGE et al, 2007a;TSUGE et al, 2007b) ou da enoil-CoA hidratase (R)-específica (TSUGE et al, 2003). Em alguns casos, foi possível sintetizar PHA contendo HA SCL e HA MCL a partir de carboidratos (FUKUI et al, 2002;NOMURA et al, 2004;QIU et al, 2005).…”

“…de PHA e a massa molar do polímero sintetizado e tem sido explorada em diferentes trabalhos (KICHISE et al, 2002;AMARA et al, 2002;TSUGE et al, 2004;TSUGE et al, 2007a;TSUGE et al, 2007b).…”