Biosynthesis and Characterizations of Silver Nanoparticles from Annona squamosa Leaf and Fruit Extracts for Size-Dependent Biomedical Applications

Malik, Maria; Iqbal, Muhammad Aamir; Malik, Mariam; Raza, Muhammad Akram; Shahid, Wajeehah; Choi, Jeong Ryeol; Pham, Phuong V.

doi:10.3390/nano12040616

Cited by 24 publications

(14 citation statements)

References 51 publications

(55 reference statements)

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“…Among the metallic NPs, various studies have reported on the synthesis of silver nanoparticles (AgNPs) using plant extracts with pharmacological potential [ 9 , 13 , 14 , 15 ]. However, the use of wild plants is not ecologically appropriate due to species decay.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Randia aculeata L. Cell Culture Extracts, Characterization, and Evaluation of Antibacterial and Antiproliferative Activity

et al. 2022

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The demand for metallic nanoparticles synthesized using green methods has increased due to their various therapeutic and clinical applications, and plant biotechnology may be a potential resource facilitating sustainable methods of AgNPs synthesis. In this study, we evaluate the capacity of extracts from Randia aculeata cell suspension culture (CSC) in the synthesis of AgNPs at different pH values, and their activity against pathogenic bacteria and cancer cells was evaluated. Using aqueous CSC extracts, AgNPs were synthesized with 10% (w/v) of fresh biomass and AgNO3 (1 mM) at a ratio of 1:1 for 24 h of incubation and constant agitation. UV-vis analysis showed a high concentration of AgNPs as the pH increased, and TEM analysis showed polydisperse nanoparticles with sizes from 10 to 90 nm. Moreover, CSC extracts produce reducing agents such as phenolic compounds (162.2 ± 27.9 mg gallic acid equivalent/100 g biomass) and flavonoids (122.07 ± 8.2 mg quercetin equivalent/100 g biomass). Notably, AgNPs had strong activity against E. coli, S. pyogenes, P. aeruginosa, S. aureus, and S. typhimurium, mainly with AgNPs at pH 6 (MIC: 1.6 to 3.9 µg/mL). AgNPs at pH 6 and 10 had a high antiproliferative effect on cancer cells (IC50 < 5.7 µg/mL). Therefore, the use of cell suspension cultures may be a sustainable option for the green synthesis of AgNPs.

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Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Randia aculeata L. Cell Culture Extracts, Characterization, and Evaluation of Antibacterial and Antiproliferative Activity

et al. 2022

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“…Changes in cancer cells may also be caused by ROS production, which causes oxidative stress and eventually leads the cell to die . Oxidative stress, caused by ROS production in tumor cells, dismantles the vital biomolecules required for proper cell function, disrupting the various physiological processes and pathways, accompanied by the destruction of important cellular organelles and bio-membranes, and eventually causing tumor cell death. , …”

Section: Resultsmentioning

confidence: 99%

“…54 Oxidative stress, caused by ROS production in tumor cells, dismantles the vital biomolecules required for proper cell function, disrupting the various physiological processes and pathways, accompanied by the destruction of important cellular organelles and bio-membranes, and eventually causing tumor cell death. 55,56 4. CONCLUSIONS C. fistula can reduce as well as stabilize the medium, which aids in producing metal NPs.…”

Section: Morphological Analysismentioning

confidence: 99%

Biosynthesizing Cassia fistula Extract-Mediated Silver Nanoparticles for MCF-7 Cell Lines Anti-Cancer Assay

et al. 2023

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The unique consequence of green synthesis is that the mediator plant is able to release chemicals that are efficacious as reducing as well as stabilizing agents. In this work, the fruit pulp and leaf essences of Cassia fistula have been used to manufacture silver nanoparticles through the green synthesis technique. The sculpturing of nanoparticles was accomplished by utilizing the reduction phenomenon that ensued due to the reaction between plant essences and the precursor solution. These biosynthesized silver nanoparticles were examined, where we used scanning electron microscopy, UV−vis spectroscopy, and X-ray diffraction techniques as means to analyze the structure, optical properties, and crystalline behavior, respectively. The absorption spectra for fruit and leaf extracts obtained from the UV−vis analyses peaked at 401 and 397 nm, and these peaks imply the appearance of optical energy gaps of 2.12 and 2.58 eV, accompanying spherical shapes of particles with diameters in the ranges of 12−20 and 50−80 nm, respectively. These silver nanoparticles together with the adopted green technique have a vast array of applications, specifically in the biomedical realm. In particular, they are being used to treat several diseases and are manifested as strong anti-tumor agents to medicate MCF-7 breast cancer cell lines in order to minimize the cell growth rate depending on their concentrations.

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“…This is due to its frequent involvement in clinical disease in these animals and its high resistance to important antimicrobials such as β-lactams, lincosamide, and uoroquinolones. This resistance is observed globally and sometimes within the EU [Malik et al 2022, Balkrishna et al 2023]. This scienti c opinion evaluates AMR in S. pseudintermedius in dogs and cats.…”

Section: Introductionmentioning

confidence: 99%

Green synthesized silver nanoparticles using Sempervivum tectorum extract have an antibacterial effect against Staphylococcus pseudintermedius isolated from a dog's otitis externa

Dégi,

Lányi,

Beteg

et al. 2024

Preprint

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Despite developing a broad spectrum of antibiotics, pathogenic bacteria remain the primary health concern. The emergence of drug-resistant strains increased therapeutic failure and mortality. The researchers are currently focusing on developing nanotechnology-based medicines to combat resistance. Our study focuses on the antimicrobial characteristics of plant-mediated silver nanoparticles (Ag NPs). The AgNPs were green synthesized (using thermal treatment) to reduce silver nitrate solution (AgNO3) using Sempervivum tectorum L. plant ethanolic aqueous extract. They were found to be broad-spectrum antimicrobial agents against two Staphylococcus pseudintermedius strains. We investigated AgNPs' antibacterial activity with time-kill curves, MIC, MBC, and well diffusion. The tests also showed a high activity of the particles against Staphylococcus pseudintermedius, with the minimum inhibitory concentrations ranging between 18.6–90.13 µg/ml. The prepared AgNPs were then characterized using scanning UV-visible spectroscopy, X-ray diffraction, and transmission electronic microscopy (TEM) to study their morphology and crystallinity. Two distinctive absorption peaks of AgNP samples were observed at 474 nm and 487 nm for leaf extract samples, with a spherical shape with face-centered cubic (FCC) crystalline structure and diameter range between 10–60 nm, respectively. The impact was significantly greater when the NPs were less than 30 nm in size. The study revealed that an ethanolic aqueous extract of Sempervivum tectorum L. may effectively reduce and stabilize silver nanoparticles (AgNPs) of various sizes, depending on the solvent concentration. The AgNPs have shown efficacy in both suppressing and eradicating microorganisms. The activity of AgNPs may be facilitated by a dual approach involving several processes of nanoparticles, such as the formation of reactive oxygen species and the presence of phytocompounds such as phenolics and proanthocyanidins bound to the nanoparticle surface. Nanotechnology-based therapies play a crucial role in treating microbial illnesses in veterinary medicine. Significant areas of research on nanoparticles (NPs) could include studying the synergistic interactions between Ag NPs and antibiotics, doing toxicity profiling, examining stability, and investigating bioavailability.

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Biosynthesis and Characterizations of Silver Nanoparticles from Annona squamosa Leaf and Fruit Extracts for Size-Dependent Biomedical Applications

Cited by 24 publications

References 51 publications

Green Synthesis of Silver Nanoparticles Using Randia aculeata L. Cell Culture Extracts, Characterization, and Evaluation of Antibacterial and Antiproliferative Activity

Green Synthesis of Silver Nanoparticles Using Randia aculeata L. Cell Culture Extracts, Characterization, and Evaluation of Antibacterial and Antiproliferative Activity

Biosynthesizing Cassia fistula Extract-Mediated Silver Nanoparticles for MCF-7 Cell Lines Anti-Cancer Assay

Green synthesized silver nanoparticles using Sempervivum tectorum extract have an antibacterial effect against Staphylococcus pseudintermedius isolated from a dog's otitis externa

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