Biosynthesis and Characterization of Silver Nanoparticles by Silybum marianum (silymarin) Fruit Extract

Abstract

This study was carried out to synthesis of silver nanoparticles (AgNPs) by using Silybummarianum fruit extract which is very simple and eco-friendly method. The separation of this nanoparticle was performed by centrifugation while the identification was by UV-Visible spectroscopy, X-ray diffraction, Fourier Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) methods. Reduction of the Ag+ to Ag0 during exposure to the S. marianum fruit extract was followed by color change of the solution from colorless, yellow to dark brown within 24 hours. It is observed that surface Plasmon resonance peaks of the maximum absorbance of silver-nanoparticles occurs at 425 nm, indicating that AgNPs were produced. Involvement of the flavonoids group (flavolignans) in the synthesized AgNPs was manifested from the result of the FTIR. Particle size was recorded according to the data exhibited from the XRD results at 2θ around 25 nm which was calculated by using the Dubai-Scherrer equation. The silver nanoparticles synthesized by the help of silymarin fruit extract were scanned using SEM. From the SEM image reveals that the silver nanoparticle seems to be spherical in shape. From the results of the current study concluded that silymarin fruit extract could be considered as a good source for synthesis of stable AgNPs in short time, and the process of synthesis was simple, low cost and eco-friendly.

Eco-friendly silver nanoparticles (AgNPs) fabricated by green synthesis using the crude extract of marine polychaete, Marphysa moribidii: biosynthesis, characterisation, and antibacterial applications

“…The colour was fully yellowish-brown ( Figure 2 (c)) after 24 h of incubation. The colour changes in the AgNPs solution was attributed to the excitation of surface Plasmon resonance (SPR), which indicates that bio-reduction of Ag + to AgNPs has confirmed the biosynthesis of AgNPs ( Ayad et al., 2018 ). Reduction of Ag + to AgNPs by the crude extract of M. moribidii occurred faster than studies of AgNPs synthesis using the crude extract of different polychaete species by Singh et al.…”

Biosynthesis, Characterization, and Anti-Inflammatory Study of Hemigraphis Colorata Loaded Silver Nanoparticles

“…Characteristic absorbance peak of AgNPs is between 400 and 450 nm. If the peak raises above 450 nm, indicates aggregation or precipitation resulting particle with large size but if peak shifts below 400 nm, the nano solution contains other participants such as impurities, organic species, and solvent [37]. The shift in absorption peak may be extremely indicative to size, morphology, quantity, and NPs growth, signifying the current study HAgNP is formed in adequate size and shape.…”

Green Synthesis of Silver Nanoparticles Using three Leaf Extracts of Ficus carica L. and evaluate its antibacterial activity against MDR Escherichia coli isolates.

“…In the present study, there were records of minutes larger than 100 nm and this was also found in previous studies such as [2] they record size of AgNPs between 100-500nm, and [11] founded size of AgNPs in range of 207-293nm. These formed sizes can be explained as due to the capping of nanoparticles by proteins [3,24] , or highly agglomerate nanoparticles resulting in aggregation of nanoparticles during sample preparation [5].…”

“…The formation of AgNPs was indicated by the color changed of the solution from pale yellow to dark brown. The formation of these nanoparticles can be tested by spectroscopic and microscopic analysis[5]. The use of biological methods in synthesizing silver nanoparticles using plant extracts is eco-friendly,Journal of Xi'an University of Architecture & Technology Volume XII, Issue III, 2020 Issn No : 1006-7930 low-cost and non-toxic compared with the chemical methods for synthesis of these nanoparticles.…”