Biosynthesis and characterisation of IgMlambda in a case of chronic lymphocytic leukaemia with intracellular immunoglobulin inclusions.

Intracellular inclusion bodies in 14 patients with B cell lymphoproliferative disorders.

“…In any case, the final prognosis of patients with CLL and inclusion bodies, does not seem to differ from that of classical CLL. This was proven by the fact that all our 12 CLL patients could be classified from stage I to stage V of the anatomicoclinical classification proposed by Binet et al 10 The clinical evolution of these patients was similar to that seen in other CLL patients.…”

Unusual intracytoplasmic immunoglobulin inclusions in chronic lymphocytic leukaemia

“…This is in keeping with the finding of small amounts of free light chains in the urine of CLL patients, including two of the three patients reported here. Conversely, 1.1 chains assembled in the cytoplasm and were not secreted during the experiment, as observed in other cases of CLL with cIg inclusions (Huhn et al, 19 75;Nies et al, 1976;Roberts et al, 1979).…”

“…The biosynthesis of Ig produced by the cells from patient 1 was studied as previously described (Preud'homme et al, 1977(Preud'homme et al, , 1980. Briefly, the cells were continuously labelled with l4C-va1ine, threonine and leucine for 3 h in depleted medium; Ig were purified from cytoplasmic extracts and secretions by indirect immunoprecipitation and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using either continuous cylindrical gels or discontinuous gels with 1 7 cm of 7.5% or 5% acrylamide at the bottom and 2 cm of 2% acrylamide gel in agarose on the top.…”

“…Preud 'homme&Seligmann, 1972'homme&Seligmann, ,1974Kaplanet al, 1972Kaplanet al, ,1973Clarket al, 1973;Cawley et al, 1973Cawley et al, , 1975Huhn et al, 1975;McCann et al, 1977;Roberts et al, 1979). In a few other cases, CLL lymphocytes contain round or oval-shaped inclusions of amorphous material contained in dilated ergastoplasmic vesicles resembling Russel bodies (Hurez et al,19 72;Nies et al,19 76).…”

Intracytoplasmic filamentous inclusions andIGHVrearrangements in a patient with chronic lymphocytic leukemia

“…Further studies [3,11] found unusual structural characteristics of the Ig and suggested that the inability of cells to secrete it might be responsible for the formation of these inclusions. In addition, biosynthetic studies have shown that intracytoplasmic Ig could precipitate as abnormal heavy chain due to a particular set of physicochemical properties, an imbalance between light and heavy chain or abnormal glycosylation [12][13][14]. More recently, Hasegawa [14] have proposed the existence of two different types of intracellular crystallization events, associated to distinct mechanisms of inclusion formation: one in the RER, related to protein synthesis, and the other in the endosome/lysosome compartments, associated to protein degradation, as was reported in plasma cell disorders [15].…”