Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies

Fernandes, Kelly Rossetti; Magri, Angela Maria Paiva; Kido, Hueliton Wilian; Parisi, Júlia Risso; Assis, Lívia; Fernandes, Kristianne Porta Santos; Mesquita‐Ferrari, Raquel Agnelli; Martins, Virgínia da Conceição Amaro; Plepis, Ana Maria de Guzzi; Zanotto, Edgar Dutra; Peitl, Oscar; Rennó, Ana Cláudia Muniz

doi:10.1016/j.jphotobiol.2017.06.002

Cited by 30 publications

(37 citation statements)

References 36 publications

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“…In the present study, the results of the histological analysis showed that the physiological inflammatory process of bone repair was already completed at 14 days in all groups due to the absence of reaction tissue, demonstrating that the association of biomaterial with both HFB and PBMT and both at the same time present biocompatibility characteristics [59,60], corroborating studies associating HFB with autogenous bone and PBMT (830 nm) [17]. Research with 830 nm [18,61] and 808 nm [62] lasers identified the modulatory action of the laser relating to mature bone neoformation with increased osteoblast factor proteins and genes, and the xenograft tested here is widely used in experiments in the literature with applications in animals and humans, with bovine bone being elected with biocompatible properties and good acceptance.…”

Section: Discussionsupporting

confidence: 83%

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

et al. 2020

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Bone defects cause aesthetic and functional changes that affect the social, economic and especially the emotional life of human beings. This complication stimulates the scientific community to investigate strategies aimed at improving bone reconstruction processes using complementary therapies. Photobiomodulation therapy (PBMT) and the use of new biomaterials, including heterologous fibrin biopolymer (HFB), are included in this challenge. The objective of the present study was to evaluate the influence of photobiomodulation therapy on bone tibial reconstruction of rats with biomaterial consisting of lyophilized bovine bone matrix (BM) associated or not with heterologous fibrin biopolymer. Thirty male rats were randomly separated into three groups of 10 animals. In all animals, after the anesthetic procedure, a noncritical tibial defect of 2 mm was performed. The groups received the following treatments: Group 1: BM + PBMT, Group 2: BM + HFB and Group 3: BM + HFB + PBMT. The animals from Groups 1 and 3 were submitted to PBMT in the immediate postoperative period and every 48 h until the day of euthanasia that occurred at 14 and 42 days. Analyses by computed microtomography (µCT) and histomorphometry showed statistical difference in the percentage of bone formation between Groups 3 (BM + HB + PBMT) and 2 (BM + HFB) (26.4% ± 1.03% and 20.0% ± 1.87%, respectively) at 14 days and at 42 days (38.2% ± 1.59% and 31.6% ± 1.33%, respectively), and at 42 days there was presence of bone with mature characteristics and organized connective tissue. The µCT demonstrated BM particles filling the defect and the deposition of new bone in the superficial region, especially in the ruptured cortical. It was concluded that the association of PBMT with HFB and BM has the potential to assist in the process of reconstructing bone defects in the tibia of rats.

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Section: Discussionsupporting

confidence: 83%

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

et al. 2020

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“…To evaluate the toxicity of PADM and LPADM to rADSCs was indirectly evaluated according to previously published method after adjustment (Fernandes et al, ), extracts from both materials were prepared by incubation in 1‐ml DMEM containing 10% FBS and 1% penicillin/streptomycin (P/S) in a 24‐well plate for 72 hr. The extract was collected and passed through a filter (Corning Costar, Cambridge, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Construction of a dermis–fat composite in vivo: Optimizing heterogeneous acellular dermal matrix with in vitro pretreatment

Zhu

Yuan

Huang

et al. 2019

J Tissue Eng Regen Med

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Dermis–fat composite tissues have been widely used in plastic and reconstructive surgery and were previously constructed using hydrogel‐type scaffolds. The constructs can be used for in vitro cosmetic and pharmaceutical testing but are not mechanically strong enough for in vivo applications. In this study, we used heterogeneous (porcine) acellular dermal matrix (PADM) as dermal layer scaffold. PADM was pretreated with the laser micropore technique and then precultured with rat adipose‐derived stem cells (rADSCs) in vitro. rADSCs proliferated well on pretreated/unpretreated PADM, showing increased expression of genes associated with inflammatory regulation, proangiogenesis, and stemness, indicating that pretreated/unpretreated PADM both provide a beneficial microenvironment for rADSCs to exert their paracrine function. After in vitro processing, the rADSCs–polyporous PADM and PADM without pretreatments were implanted into the back of rats respectively, followed by adipose tissue transplantation. After implantation, the inflammation induced by pretreated PADM was significantly attenuated and localized compared to the unpretreated group. Moreover, the vascularization was faster, and more adipose tissue was formed in the pretreated group. Sound dermis–fat composite tissue was constructed with sufficient strength, which can potentially be used for actual repair application.

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“…The positive biostimulating effect of LLLI has also been reported for cells cultured on biomaterial surfaces, including urethane polycarbonate (Hsu, Fu, Huang, Tsai, & Yen, 2010), biosilicate (Fernandes et al, 2017), and acellular dermal matrix (Choi et al, 2013). Additionally, LLLI has been proposed as the fourth element of tissue engineering for improving the triad of tissue engineering along with cells, scaffolds, and growth factors (Marques et al, 2016).…”

Section: Introductionmentioning

confidence: 79%

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

Sabino

Ginani

Silva

et al. 2020

J Tissue Eng Regen Med

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This study aimed to verify the efficacy of low‐level laser irradiation (LLLI) on the proliferation of MC3T3‐E1 preosteoblasts cultured on poly(lactic acid) (PLA) films. The produced films were characterized by contact angle tests, scanning electron microscopy (SEM), atomic force microscopy, differential scanning calorimetry, and X‐ray diffraction. The MC3T3‐E1 cells were cultured as three different groups: Control—cultured on polystyrene plastic surfaces; PLA—cultured on PLA films; and PLA + Laser—cultured on PLA films and submitted to laser irradiation (660 nm; 30 mW; 4 J/cm2). Cell proliferation was analyzed by Trypan blue and Alamar blue assays at 24, 48, and 72 h after irradiation. Cell viability was assessed by Live/Dead assay, apoptosis‐related events were evaluated by Annexin V/propidium iodide (PI) expression, and cell cycle events were analyzed by flow cytometry. Cell morphology on the surface of films was assessed by SEM. Cell counting and biochemical assay results indicate that the PLA + Laser group exhibited higher proliferation (p < 0.01) when compared with the Control and PLA groups. The Live/Dead and Annexin/PI assays indicate increased cell viability in the PLA + Laser group that also presented a higher percentage of cells in the proliferative cell cycle phases (S and G2/M). These findings were also confirmed by the higher cell density observed in the irradiated group through SEM images. The evidence from this study supports the idea that LLLI increases the proliferation of MC3T3‐E1 cells on PLA surfaces, suggesting that it can be potentially applied in bone tissue engineering.

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Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies

Cited by 30 publications

References 36 publications

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

Construction of a dermis–fat composite in vivo: Optimizing heterogeneous acellular dermal matrix with in vitro pretreatment

Laser therapy increases the proliferation of preosteoblastic MC3T3‐E1 cells cultured on poly(lactic acid) films

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