Bioserotypes and Virulence Markers of Yersinia enterocolitica Strains Isolated from Mallards (Anas platyrhynchos) and Pheasants (Phasianus colchicus)

Free-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis -foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans.

mentioning

confidence: 52%

Section: Primers and Triplex Pcr Conditionsmentioning

confidence: 99%

Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)

Bancerz‐Kisiel¹,

Szczerba‐Turek²,

Platt-Samoraj³

et al. 2014

“…The details of the bacteriological procedure and serotype and biotype determination methods were previously described by Bancerz-Kisiel et al (2012).…”

Section: Methodsmentioning

confidence: 99%

“…Based on differences of the antigen O structure, Y. enterocolitica was divided into over 70 serotypes belonging to 6 biotypes, 1A, 1B, 2-5 (Bottone 1997). Most of Y. enterocolitica strains isolated so far from free-living animals or from the environment belong to biotype 1A, concerned as an nonpathogenic (Bancerz-Kisiel et al 2012). Currently, strains of that biotype are increasingly often isolated from clinical cases of yersiniosis but the pathogenicity of these strains is difficult to define (Ramamurthy et.…”

Section: Introductionmentioning

confidence: 99%

Yersinia enterocolitica strains isolated from beavers (Castor fiber)

Platt-Samoraj¹,

Syczyło²,

Bancerz‐Kisiel³

et al. 2015

Pseudocloacal swabs and palatine tonsils from beavers have been examined for the Yersinia enterocolitica presence. Thirty-six samples from 24 beavers were collected and subjected to bacteriological examinations including sero-and biotypisation. Amplicons confirmed by PCR as Y. enterocolitica were sequenced. Positive samples originated from 4 out of the 24 beavers (16.7 %) and all the strains belonged to biotype 1A. The study suggested that Y. enterocolitica could be isolated from beavers, which may therefore be treated as a reservoir, a significant factor of water contamination and a vector of the Y. enterocolitica.

“…Wildlife birds can be a reservoir of various pathogens that infect farm animals and humans (Stenzel et al 2008, Benskin et al 2009, Bancerz-Kisiel et al 2012). Despite the above, very little is known about the incidence of Bordetella avium in populations of wildlife birds.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bordetella avium by TaqMan real-time PCR in tracheal swabs from wildlife birds

Stenzel¹,

Pestka²,

Tykałowski³

et al. 2017

Bordetella avium, the causing agent of bordetellosis, a highly contagious infection of the respiratory tract in young poultry, causes significant losses in poultry farming throughout the world. Wildlife birds can be a reservoir of various pathogens that infect farm animals. For this reason the studies were conducted to estimate the prevalence of Bordetella avium in wildlife birds in Poland. Tracheal swab samples were collected from 650 birds representing 27 species. The bacterial DNA was isolated directly from the swabs and screened for Bordetella avium by TaqMan real-time PCR.The assay specificity was evaluated by testing DNA isolated from 8 other bacteria that can be present in avian respiratory tract, and there was no amplification from non-Bordetella avium agents. Test sensitivity was determined by preparing standard tenfold serial dilutions of DNA isolated from positive control. The assay revealed to be sensitive, with detection limit of approximately 4.07x10^2 copies of Bordetella avium DNA. The genetic material of Bordetella avium was found in 54.54% of common pheasants, in 9.09% of Eurasian coots, in 3.22% of black-headed gulls and in 2.77% of mallard ducks.The results of this study point to low prevalence of Bordetella avium infections in wildlife birds. The results also show that described molecular assay proved to be suitable for the rapid diagnosis of bordetellosis in the routine diagnostic laboratory.