Biosensors Made of Synthetic Functional Nucleic Acids Toward Better Human Health

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

“…54,80 DNA aptamers and deoxyribozymes (DNAzymes), which are chemically more stable and easier to handle than their RNA counterparts, have been frequently used. [83][84][85][86][87][88] CRISPR-Cas12 has been used for non-nucleic acid target detection because Cas12 targets both dsDNA and ssDNA and has the additional benet of trans-cleavage activity upon target binding. 13 Molecular translators other than aptamers and DNAzymes exist and can also convert the detection of non-nucleic acid targets into surrogate nucleic acid targets that can be detected by CRISPR-Cas techniques.…”

A serological aptamer-assisted proximity ligation assay for COVID-19 diagnosis and seeking neutralizing aptamers

“…Compared with antibodies, DNA aptamers, known as "chemical antibodies", are a powerful class of recognition agents with several advantages of high specicity and affinity, rapid and reliable synthesis, ease of conjugation, and good feasibility to integrate with other DNA-based reactions for signal amplication. [21][22][23][24][25] These advantages enable aptamers to be a promising receptor, with diverse applications for in vitro and in vivo diagnosis. [26][27][28][29][30][31][32][33][34][35][36][37][38][39] In addition to the choice of receptor, it is also important to develop amplication methods to detect COVID-19 with high sensitivity.…”

Selection and applications of functional nucleic acids for infectious disease detection and prevention

“…The most commonly used FNAs are aptamers and DNAzymes (Fig. 1), which are DNA or RNA molecules selected from Published in the topical collection Analytical Chemistry for Infectious Disease Detection and Prevention with guest editors Chaoyong Yang and XiuJun (James) Li. in vitro screening and confirmed with high binding affinity and selectivity against targets [4]. Compared with antibodies, FNAs promise several advantages.…”