Biosensors for the determination of environmental inhibitors of enzymes

Evtugyn, Gennady; Budnikov, H. C.; Nikol'skaya, E. B.

doi:10.1070/rc1999v068n12abeh000525

Cited by 20 publications

(6 citation statements)

References 131 publications

(127 reference statements)

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“…4 In this work, a value of 10% inhibition used as the detection limit corresponds to 1.0-1.6 ng ml 21 . It should be noted that compared with other, previous, methods for the determination of mercury based on the inhibition of different enzymes, [3][4][5][6][7]33,34 invertase is the most sensitive enzyme to mercury with the best detection limit achieved (1.0 ng ml 21 ). A lower detection limit (0.2 ng ml 21 ) was recently reported with glucose oxidase and the mediator 2-aminoethanethiol, but in that case Hg(II) reacts with thiol rather than with enzyme.…”

Section: Gox-modified Platinum Electrodementioning

confidence: 86%

Determination of mercury(ii) by invertase enzyme inhibition coupled with batch injection analysis

Mohammadi

Rhazi

Amine

et al. 2002

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The determination of mercury(II) ions at the trace level by inhibition of the invertase enzyme-catalysed hydrolysis of sucrose into glucose and fructose coupled to electrochemical batch injection analysis was investigated using two approaches. In the first, the glucose produced was detected by injection of 100 microliters samples into the batch injection cell containing a platinum electrode modified by immobilised glucose oxidase. In the second, the glucose and fructose present in injected samples were oxidised directly at a copper-modified glassy carbon electrode. The experimental parameters were optimised and the degree of enzyme inhibition by mercury(II) ions under both conditions was measured. Mercury concentrations in the ng ml-1 range were determined by these two techniques with low sample and reagent consumption. Comparison is made between the two methods and perspectives as a screening test for field application are indicated.

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Section: Gox-modified Platinum Electrodementioning

confidence: 86%

Determination of mercury(ii) by invertase enzyme inhibition coupled with batch injection analysis

Mohammadi

Rhazi

Amine

et al. 2002

Analyst

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“…The enzyme and transducer elements are in close contact with each other and incorporated in a single unit. Some biosensors based on enzyme inhibition have been reported in the literature (Tran-Minh, 1985;Evtugyn et al, 1999;Luque de Castro and Herrera, 2003).…”

Section: Introductionmentioning

confidence: 99%

Enzyme inhibition-based biosensors for food safety and environmental monitoring

Amine¹,

Mohammadi²,

Bourais³

et al. 2006

Biosensors and Bioelectronics

549

263

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“…Additonally, many enzymes need cofactors for the detection of substances (http://www.rpi.edu/dept/chem-eng/Biotech Environ/BIOSEN/enzbio.htm). Biosensors for heavy metals have been mainly developed in environmental analysis in water (Evtugyn et al 1999). As far as enzyme biosensors for heavy metal determination are concerned, a certain number of papers have appeared, reporting the use of different enzymes and biosensor configurations/transducers , Compagnone et al 1995and Donlan et al 1989b, Starodub et al 1999, Vel Krawczyc et al 2000, Pirvutoiu et al 2001, and Dzyadevych et al 2003.…”

Section: Lead Toxicity and δ-Alad As Biosensor For Lead Toxicitymentioning

confidence: 99%