Biosensors for phytohormone Abscisic acid and its role in humans: A review

Subodh, .; Ravina,; Choudhary, Priyanka; Narang, Jagriti; Mohan, Hari

doi:10.1016/j.sintl.2023.100234

Cited by 3 publications

(2 citation statements)

References 110 publications

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“…Recently, scientists have developed genetically encoded FRET sensors for the purpose of detecting ABA concentration and uptake in plants. These sensors demonstrate a high signal-to-noise ratio and enable precise measurements of ABA levels in living tissue [83].…”

Section: Aba Quantification Techniquesmentioning

confidence: 99%

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Role of Abscisic Acid in Plant Stress

Sharma,

Sharma

2023

New Insights Into Phytohormones

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The multifaceted role of Abscisic acid (ABA) as a phytohormone of great repute cannot be overstated. ABA right after its synthesis within plastids embark on a quest to find specific receptors. On binding these receptors a complex signaling cascade is triggered that ultimately modulates gene expression and other cellular processes, responsible for normal growth and development processes of plants. Under abiotic and biotic stresses ABA levels change tremendously, triggering a cascade of physiological responses that help the plant adapt to its environment. A deeper understanding of ABA’s mechanisms like understanding its metabolic pathways or its regulation at genetic and epigenetic levels hold the promise of enhancing crop productivity and resilience in the face of the daunting challenges posed by a changing climate. Use of gene editing techniques like CRISPER-Cas technology, regulating the ABA mediated stress responsive genes, using RNAi and modifying the intragenic and promoter regions of the genes involved in ABA biosynthesis are a few methods which can enhance the ABA production or ABA mediated response to tolerate the stress conditions. In essence, ABA is a paramount player in plant stress responses, and unlocking its mysteries holds the potential to revolutionize agriculture and safeguard food security.

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Section: Aba Quantification Techniquesmentioning

confidence: 99%

“…When the sensor closes, fluorescent proteins with improved dimerization propensities will improve the sensor's closed state through dimerization. Additionally, a high FRET efficiency is anticipated as a result of the heterodimer state's parallel orientation [83].…”

Section: Aba Quantification Techniquesmentioning

confidence: 99%

Role of Abscisic Acid in Plant Stress

Sharma,

Sharma

2023

New Insights Into Phytohormones

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Implications in Phytohormone Research: From Lab to Field

Arif,

Mustafa,

Cheng

2024

Plant Growth Regulators: Resilience for Sustainable Agriculture

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Quantifying Plant Signaling Pathways by Integrating Luminescence-Based Biosensors and Mathematical Modeling

Ahmed,

Naqvi,

Hussain

et al. 2024

Biosensors

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Plants have evolved intricate signaling pathways, which operate as networks governed by feedback to deal with stressors. Nevertheless, the sophisticated molecular mechanisms underlying these routes still need to be comprehended, and experimental validation poses significant challenges and expenses. Consequently, computational hypothesis evaluation gains prominence in understanding plant signaling dynamics. Biosensors are genetically modified to emit light when exposed to a particular hormone, such as abscisic acid (ABA), enabling quantification. We developed computational models to simulate the relationship between ABA concentrations and bioluminescent sensors utilizing the Hill equation and ordinary differential equations (ODEs), aiding better hypothesis development regarding plant signaling. Based on simulation results, the luminescence intensity was recorded for a concentration of 47.646 RLUs for 1.5 μmol, given the specified parameters and model assumptions. This method enhances our understanding of plant signaling pathways at the cellular level, offering significant benefits to the scientific community in a cost-effective manner. The alignment of these computational predictions with experimental results emphasizes the robustness of our approach, providing a cost-effective means to validate mathematical models empirically. The research intended to correlate the bioluminescence of biosensors with plant signaling and its mathematical models for quantified detection of specific plant hormone ABA.

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Biosensors for phytohormone Abscisic acid and its role in humans: A review

Cited by 3 publications

References 110 publications

Role of Abscisic Acid in Plant Stress

Role of Abscisic Acid in Plant Stress

Implications in Phytohormone Research: From Lab to Field

Quantifying Plant Signaling Pathways by Integrating Luminescence-Based Biosensors and Mathematical Modeling

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