Biosensors for food analysis

Warsinke, Axel

doi:10.1007/978-3-0348-9045-8_9

Cited by 11 publications

(5 citation statements)

References 57 publications

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“…Электрохимические сенсоры -это устройства, в которых аналитический сигнал обеспечивается протеканием электрохимической реакции в приэлектродном пространстве. Предназначены для качественного и количественного анализа жидких и газообразных сред [4][5][6].…”

Section: материалы и методыunclassified

Laser synthesis of nanomaterials to create a new family of electrochemical microbiosensors

et al. 2020

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A brief review of modern methods for creating materials for enzymeless microbiosensors intended for express analysis of the content of components of biological fluids, including human blood, has been made. New directions of the synthesis of such materials have been described: laser ablation (PLD) and laser-induced deposition (LCLD). The comparison of laser methods for the synthesis of materials of non-enzymatic microbiosensors with the known methods for creating nanostructured materials has been carried out. Using bimetallic LCLD microtracks as an example, the mechanism of enhancing the electrochemical response of the sensor to the content of glucose and hydrogen peroxide in complex organic and biological mixtures has been shown. It is associated with the creation of nano- and microstructured materials with a highly developed surface, on which there are extended boundaries of the interphase contact zones. This creates numerous activated acid-base centers. They facilitate the transfer of charge from the oxidizing agent to the reducing agent in the solution in contact with the sensor surface. A comparison of the sensory properties of microcomposite bimetallic deposits synthesized by the laser method and their analogs synthesized by traditional methods has been carried out. The advantages of laser methods for the synthesis of microcomposite sensor-active materials are discussed: the miniature size of the sensors, the possibility of using inexpensive metals instead of precious ones, the environmental friendliness of the methods, and the absence of the need to pre-activate the surface

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Section: материалы и методыunclassified

Laser synthesis of nanomaterials to create a new family of electrochemical microbiosensors

et al. 2020

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“…In addition, antibodies have been generated against a wide array of bacterial pathogens. Immuno-sensors have been shown to exhibit faster speed and lower cost compared to standard culture detection methods and DNA-based biorecognition techniques, making them especially marketable to the food industry [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of 3-Thiopheneacetic Acid in the Polymerization of a Conductive Electrotextile for Use in Biosensor Development

et al. 2013

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Investigations were conducted to develop an electrotextile using a nonwoven polypropylene fiber platform conformally coated in a conductive, functionalized copolymer of polypyrrole and 3-thiopheneacetic acid (3TAA). The objectives of this study were to determine: (1) if the inclusion of 3TAA in the polymerization process would have an effect on the availability of binding sites in the high-surface area electrotextile for biorecognition elements and (2) how the increase in the concentration of 3TAA would affect the physical characteristics of the coating, resistivity of the sample and availability of binding sites. It was found that the addition of 3TAA to the polymerization process resulted in an increase in the size of the polypyrrole coating, as well as the material resistivity and available binding sites for biorecognition elements. These factors were used to determine which of the tested concentrations was best for biosensor development. A polymer coated membrane sample containing a concentration within the range of 10–50 mg/mL of 3TAA was selected as the best for future biosensor work.

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“…In addition, antibodies can be generated against nearly any bacterial pathogen. The faster speed and lower cost of immunobiosensors versus standard detection methods and biosensors using other biorecognition techniques, such as DNA, have made them especially marketable for use in food matrices [7][8][9]. In an electrochemical biosensor, the biological recognition element is immobilized on an electrode, which then converts the biological recognition event (i.e.…”

Section: Introductionmentioning

confidence: 99%

A Resistance Based Biosensor That Utilizes Conductive Microfibers for Microbial Pathogen Detection

McGraw¹,

Alocilja²,

Senecal³

et al. 2012

OJAB

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Escherichia coli O157:H7 (E. coli O157:H7) is one of the top pathogens of interest for the development of rapid diagnostic systems for food and water samples. The objective of this research is to develop a rapid, novel electrochemical biosensor based on the use of polypropylene microfiber membranes coated with a conductive polypyrrole and antibody functionalized for the biological capture and detection of E. coli O157:H7 in the field. Using glutaraldehyde, pathogen specific antibodies are covalently attached to conductive microfiber membranes which are then blocked using a 5% bovine serum albumin solution. The functionalized membranes are then exposed to E. coli O157:H7 cells washed in Butterfield's phosphate buffer and added to a phosphate-buffer electrolyte solution. When a voltage is applied to the system, the presence of the captured pathogen on the fiber surface results in an increase in resistance at the electrotextile electrode surface, indicating a positive result. In this study, the initial resistance of the membrane in the electrochemical system was established and found to range between 5.8 kΩ and 13 kΩ. The resistance of the system not associated with the electrotextile fibers was calculated to contribute to only 2.8% of the total system resistance, and found not to be significant. A proof of concept experiment was conducted and determined that the electrotextile electrode was able to differentiate between small changes in a solution's conductivity associated with the presence of E. coli O157:H7 cells over a concentration range of log 0-9 CFU/mL.

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Biosensors for food analysis

Cited by 11 publications

References 57 publications

Laser synthesis of nanomaterials to create a new family of electrochemical microbiosensors

Laser synthesis of nanomaterials to create a new family of electrochemical microbiosensors

The Effect of 3-Thiopheneacetic Acid in the Polymerization of a Conductive Electrotextile for Use in Biosensor Development

A Resistance Based Biosensor That Utilizes Conductive Microfibers for Microbial Pathogen Detection

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