Biosensors based on enzyme inhibition: Detection of organophosphorus and carbamate insecticides and dithiocarbamate fungicides

Abstract

The dithiocarbamate fungicides and the organophosphorus and carbamate insecticides are two families of pesticides of environmental concern used on a very large scale worldwide. The need for increasing numbers of analyses has created a demand for the development of rapid, simple, and low cost methods. Enzyme sensors based on the abilities of these compounds to inhibit enzymes are described. An enzyme sensor with immobilized aldehyde dehydrogenase is described allowing the detection of 9 ppb of zineb, whereas the classical method allows the determination of 400 ppb. Using screen‐printed electrodes with acetylcholinesterase, it is possible to detect 0.5 ppb of paraoxon in aqueous buffer and 1.4 ppb of paraoxon in a mixture of hexane and water (80/20). © 1999 John Wiley & Sons, Inc. Field Analyt Chem Technol 3: 171–178, 1999

Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations

“…This is a very common strategy used by our group to immobilize biocatalysts on electrodes made from different materials, by use of different types of poly(vinyl alcohol) bearing styryl pyridinium groups [32,33,34,35]. With screenprinted graphite electrodes this strategy was applied for the construction of biosensors for D-lactic acid [26], acetaldehyde [26], dithiocarbamate fungicides [36], and organophosphorus and carbamate pesticides.…”

Organophosphorus Pesticides Determination by Electrochemical Biosensors

“…These inhibition based acylcholinesterases sensor for OP quantification have been extensively reviewed (Andreescu & Marty, 2006;Anzai, 2006;Jaffrezic-Renault, 2001;Noguer et al, 1999;Prieto-Simón et al, 2006;Rodriguez-Mozaz et al, 2004;Solé et al, 2003a;Solé et al, 2003b;Tran-Minh, 1985;Turdean et al, 2002). Attention has been paid to two main types of them, according to the transduction mode: the potentiometric and the amperometric ones.…”

Enzyme vs. Bacterial Electrochemical Sensors for Organophosphorus Pesticides Quantification

“…An important drawback represents the complex, long-lasting, and expensive procedure for OPH or PH extraction and purification, performed in specialized microbiological laboratories (to note that these enzymes are not commercially available) (Prieto-Simón et al, 2006). Some reviews summarize the performances of the enzyme electrochemical sensors for OP pesticides determination and the principles of their operation Anzai, 2006;Jaffrezic-Renault, 2001;Mazzei et al, 1996;Mulchandani et al, 2001a;Noguer et al, 1999;Prieto-Simón et al, 2006;Rodriguez-Mozaz et al, 2004;Solé et al, 2003a;Solé et al, 2003b;Tran-Minh, 1985;Turdean et al, 2002). Selected relevant data, demonstrating the sensitivity of the enzyme sensors are given in Table 1 and Table 2.…”