Biosensor system for continuous monitoring of organophosphate aerosols

Luckarift, Heather R.; Greenwald, Roby; Bergin, Michael; Spain, Jim C.; Johnson, Glenn R.

doi:10.1016/j.bios.2007.04.023

Cited by 26 publications

(31 citation statements)

References 22 publications

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“…Further investigations using R5 for biosilica formation have led to immobilization of a variety of enzymes for sensor capabilities, catalytic applications, and novel biomaterial production. [29][30][31][32][33][34][35][36][37][38][39] In a few cases, the applicable enzymes themselves catalyze biomineralization reactions, which result in their self-immobilization and retention of activity within the inorganic matrix without addition of a separate biomineralizing peptide. 40,41 Whether part of a physiological function or of man-made design, many peptides that catalyze biomineralization reactions (e.g., histidine-rich proteins, [42][43][44] noble metal nanoparticle-forming peptides, 45,46 silaffins, silicatiens, and other lysine-rich proteins 47 ) have an overall positive charge and are amphiphilic in nature; characteristics that are also shared with many AMPs.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

2008

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Amphiphilicity and cationicity are properties shared between antimicrobial peptides and proteins that catalyze biomineralization reactions. Merging these two functionalities, we demonstrate a reaction where a cationic antimicrobial peptide catalyzes self-biomineralization within inorganic matrices. The resultant antimicrobial peptide nanoparticles retain biocidal activity, protect the peptide from proteolytic degradation, and facilitate a continuous release of the antibiotic over time. Taken together, these properties demonstrate the therapeutic potential of selfsynthesizing biomaterials that retain the biocidal properties of antimicrobial peptides.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

2008

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“…Another type of immobilisation is the enzyme entrapment in matrix [99][100][101][102][103]. Andreescu et al have reported the immobilisation of ChE by encapsulation in sol-gel prepared by TMSO (Tetramethoxysilane) and MTMSOS (Methyltrimethoxysilane) or by entrapment in poly(vinylalcohol) bearing styrylpyridinium (PVA-SbQ) showing in both cases a storage stability of several months [104].…”

Section: Immobilisationmentioning

confidence: 99%

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

et al. 2010

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The present review reports the research carried out during last 9 years on biosensors based on cholinesterase inhibition for nerve agents, organophosphorus and carbammic insecticides, and aflatoxin B 1 detection. Relative applications in environmental and food areas are also reported. Special attention is paid to the optimization of parameters such as enzyme immobilization, substrate concentration, and incubation time in the case of reversible inhibition by aflatoxin B 1 or irreversible inhibition by organophosphorus and carbamic insecticides, and nerve agents in order to optimize and improve the analytical performances of the biosensor. Evaluation of selectivity of the system is also discussed.

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“…A matrix of spherical silica particles (typically ~500nm in diameter) is formed and provides effective encapsulation of a range of enzymes including esterases, hydrolases, peroxidases and reductases. [23,35,[56][57][58][59] The silica particles can be further integrated into flow-through systems by either: i) preparing the silica particles in batch mode and then packing them into a column or by ii) preparing the silica particles within a commercially available pre-packed column, in situ, by anchoring them to the column matrix.…”

Section: Biosilica For Imer Preparationmentioning

confidence: 99%

“…A number of examples of such are included in a review by Fishman et al [116] The IMER containing biosilica-immobilized BuChE described earlier for screening the drug potency of cholinesterase inhibitors, for example, was also integrated with an aerosol collection system to develop a biosensor for the detection of organophosphate nerve agents in air. [59] The system was tested with model organophosphates including paraoxon, demeton-S and malathion. The substrates are all potent inhibitors of BuChE and as such, the BuChE-IMER required reactivation with pyridine-2-aldoxime following every test sample in order to maintain enzymatic activity over repeated use.…”

Section: Imers As Biosensorsmentioning

confidence: 99%

Silica-Immobilized Enzyme Reactors

Luckarift¹

2008

Journal of Liquid Chromatography & Related Technologies

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Standard Form 298 (Rev. 8/98) REPORT DOCUMENTATION PAGEPrescribed by ANSI Std. Z39.18 Form Approved OMB No. 0704-0188The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, The effect of various silica matrices and immobilization techniques upon the enzymatic properties and stability of the biocatalysts is considered. In addition, reports in which the carrier matrix is biologically-derived silica are highlighted as an alternative and versatile technique that provides advantageous recovery, reuse and reproducibility. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) SPONSOR/MONITOR'S REPORT NUMBER(S)

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Biosensor system for continuous monitoring of organophosphate aerosols

Cited by 26 publications

References 22 publications

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

Synthesis of Bioinorganic Antimicrobial Peptide Nanoparticles with Potential Therapeutic Properties

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

Silica-Immobilized Enzyme Reactors

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