Biosensor for calcium based on a hydrogel optical waveguide with integrated sensor proteins

Forster, Thilo; Strohhöfer, C.; Bock, Karlheinz; Kasák, Peter; Danko, Martin; Kroneková, Zuzana; Nedelčev, Tomáš; Krupa, Igor; Lacı́k, Igor

doi:10.1109/sensor.2009.5285877

Cited by 6 publications

(5 citation statements)

References 2 publications

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“…(b) Photograph of a fiber piece guiding 633 nm red light across the 15 cm fiber length. (c) Comparison of BOF attenuations per monitoring wavelength from the literature including cellulose-based (blue 1–4), − Alg or agarose-based (red, 5–11), ,,,− ,, silk-based (orange 12–16), ,,,, PLA or gelatin-based (purple, 17–19), − and MC-Alg-based fibers studied in this work (20–27).…”

Section: Resultsmentioning

confidence: 99%

Mechanically Robust Biopolymer Optical Fibers with Enhanced Performance in the Near-Infrared Region

Patrakka,

Hynninen,

Lahtinen

et al. 2024

ACS Appl. Mater. Interfaces

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Polymer optical fibers (POFs) are lightweight, fatigue-tolerant, and suitable for local area networks, automobiles, aerospace, smart textiles, supercomputers, and servers. However, commercially available POFs are exclusively fabricated using synthetic polymers derived from nonrenewable resources. Recently, attempts have been made to fabricate biocompatible and biopolymeric optical fibers. However, their limitations in mechanical performance, thermal stability, and optical properties foil practical applications in waveguiding. Here, we report a comprehensive study of the preparation of biopolymer optical fibers with tailored mechanical strength, thermal properties, and their short-distance applications. Specifically, we use alginate as one of the key components with methylcelluloses to promote readily scalable wet spinning at ambient conditions to fabricate 21 combinations of composite fibers. The fibers display high maximum strain (up to 58%), Young's modulus (up to 11 GPa), modulus of toughness (up to 63 MJ/m 3 ), and a high strength (up to 195 MPa), depending on the composition and fabrication conditions. The modulus of toughness is comparable to that of glass optical fibers, while the maximum strain is nearly 15 times higher. The mechanically robust fibers with high thermal stability allow rapid humidity, touch sensing, and complex shapes such as serpentine, coil, or twisted structures without losing their light transmission properties. More importantly, the fibers display enhanced optical performance and sensitivity in the near-infrared (NIR) region, making them suitable for advanced biomedical applications. Our work suggests that biobased materials offer innovative solutions to create short-distance optical fibers from fossil fuel-free resources with novel functionalities.

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Section: Resultsmentioning

confidence: 99%

Mechanically Robust Biopolymer Optical Fibers with Enhanced Performance in the Near-Infrared Region

Patrakka,

Hynninen,

Lahtinen

et al. 2024

ACS Appl. Mater. Interfaces

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“…The cell-containing hydrogel waveguide was implanted in living mice, where toxic QDs (CdTe; CdSe/ZnS) could be detected from the fluorescence signals of the sensing cells (Figure 5a,b). Forster et al presented an implantable hydrogel waveguide with integrated fluorescent proteins for calcium detection, where the calcium ions bond with the immobilized proteins, resulting in fluorescence changes due to the fluorescence resonance energy transfer effect [74]. Also, implantable optical waveguides coupled with fluorescence sensing have been exploited as optical neural interfaces for studying neural activity in vivo [33,52].…”

Section: Applications Of Polymeric Optical Waveguide-based Sensorsmentioning

confidence: 99%

Soft and Stretchable Polymeric Optical Waveguide-Based Sensors for Wearable and Biomedical Applications

Guo

Yang

Dai

et al. 2019

Sensors

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The past decades have witnessed the rapid development in soft, stretchable, and biocompatible devices for applications in biomedical monitoring, personal healthcare, and human–machine interfaces. In particular, the design of soft devices in optics has attracted tremendous interests attributed to their distinct advantages such as inherent electrical safety, high stability in long-term operation, potential to be miniaturized, and free of electromagnetic interferences. As the alternatives to conventional rigid optical waveguides, considerable efforts have been made to develop light-guiding devices by using various transparent and elastic polymers, which offer desired physiomechanical properties and enable wearable/implantable applications in optical sensing, diagnostics, and therapy. Here, we review recent progress in soft and stretchable optical waveguides and sensors, including advanced structural design, fabrication strategies, and functionalities. Furthermore, the potential applications of those optical devices for various wearable and biomedical applications are discussed. It is expected that the newly emerged soft and stretchable optical technologies will provide a safe and reliable alternative to next-generation, smart wearables and healthcare devices.

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“…Despite the focus of this paper is on the electronics, an overview of the complete system is provided in this section, while a more detailed description is reported in [21]. The complete system is conceived to work as an under-skin implant, detecting analytes in interstitial fluids.…”

Section: A Sensing Principlementioning

confidence: 99%

“…Preliminary functionality tests were carried out with the electronic platform and a hydrogel waveguide biosensor [21] containing glucose binding proteins [29], [30] in the FRET compound molecule.…”

Section: A In Vitro Functionality Testmentioning

confidence: 99%

Wireless Implantable Electronic Platform for Chronic Fluorescent-Based Biosensors

Valdastri

Susilo

Forster

et al. 2011

IEEE Trans. Biomed. Eng.

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Abstract-The development of a long-term wireless implantable biosensor based on fluorescence intensity measurement poses a number of technical challenges, ranging from biocompatibility to sensor stability over time. One of these challenges is the design of a power efficient and miniaturized electronics, enabling the biosensor to move from bench testing to long term validation, up to its final application in human beings. In this spirit, we present a wireless programmable electronic platform for implantable chronic monitoring of fluorescent-based autonomous biosensors. This system is able to achieve extremely low power operation with bidirectional telemetry, based on the IEEE802.15.4-2003 protocol, thus enabling over three-year battery lifetime and wireless networking of multiple sensors. During the performance of single fluorescent-based sensor measurements, the circuit drives a laser diode, for sensor excitation, and acquires the amplified signals from four different photodetectors. In vitro functionality was preliminarily tested for both glucose and calcium monitoring, simply by changing the analyte-binding protein of the biosensor. Electronics performance was assessed in terms of timing, power consumption, tissue exposure to electromagnetic fields, and in vivo wireless connectivity. The final goal of the presented platform is to be integrated in a complete system for blood glucose level monitoring that may be implanted for at least one year under the skin of diabetic patients. Results reported in this paper may be applied to a wide variety of biosensors based on fluorescence intensity measurement.Index Terms-Biotelemetry, calcium sensing, chronic implants, fluorescence-based biosensor, fluorescence resonant energy transfer (FRET), glucose monitoring, implantable devices.

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Biosensor for calcium based on a hydrogel optical waveguide with integrated sensor proteins

Cited by 6 publications

References 2 publications

Mechanically Robust Biopolymer Optical Fibers with Enhanced Performance in the Near-Infrared Region

Mechanically Robust Biopolymer Optical Fibers with Enhanced Performance in the Near-Infrared Region

Soft and Stretchable Polymeric Optical Waveguide-Based Sensors for Wearable and Biomedical Applications

Wireless Implantable Electronic Platform for Chronic Fluorescent-Based Biosensors

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