Biosensor based on hydrogel optical waveguide spectroscopy

Wang, Yi; Huang, Chun Jen; Jonas, Ulrich; Wei, Tianxin; Dostálek, Jakub; Knoll, Wolfgang

doi:10.1016/j.bios.2009.12.003

Cited by 86 publications

(68 citation statements)

References 27 publications

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“…The use of hydrogels as waveguides has also been investigated for sensing of various biomolecules [6,7]. Many hydrogels are amenable to cell encapsulation which can then be used for light-mediated sensing or drug release [4,8].…”

Section: Introductionmentioning

confidence: 99%

Biocompatible silk step-index optical waveguides

Applegate¹,

Perotto²,

Kaplan³

et al. 2015

Biomed. Opt. Express

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Biocompatible optical waveguides were constructed entirely of silk fibroin. A silk film (n=1.54) was encapsulated within a silk hydrogel (n=1.34) to form a robust and biocompatible waveguide. Such waveguides were made using only biologically and environmentally friendly materials without the use of harsh solvents. Light was coupled into the silk waveguides by direct incorporation of a glass optical fiber. These waveguides are extremely flexible, and strong enough to survive handling and manipulation. Cutback measurements showed propagation losses of approximately 2 dB/cm. The silk waveguides were found to be capable of guiding light through biological tissue. #246610Received 23

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Section: Introductionmentioning

confidence: 99%

Biocompatible silk step-index optical waveguides

Applegate¹,

Perotto²,

Kaplan³

et al. 2015

Biomed. Opt. Express

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“…Such FOM is more than twofold better than that for regular SPR at the same wavelength (FOM = 20.8 RIU −1 ) but it is about 15 times lower than that observed for similar pNIPAAm hydrogel waveguide that was modifi ed with small biomolecules (FOM = 810 RIU −1 ). [ 21 ] The reason is that the loading of nanoMIPs or nanoNIPs into the waveguide leads to the increased imaginary part of the refractive index Im{ n h } (due to the scattering of the guided mode on nanoparticles) and subsequently to higher resonance width θ r (increased by a factor of about fi ve). In addition, the presence of nanoMIP increases the total polymer volume fraction f and thus smaller volume accessible for diffusing of analyte (sensitivity S and accessible volume proportional to (1 − f ) is decreased by a factor of 2-3 with respect to the structure without nanoMIP).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, swollen hydrogel nanoMIP composite fi lm can simultaneously serve as a waveguide, which confi nes probing optical fi eld in the region where specifi c molecular binding events occur. [ 21,22 ] …”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low‐Molecular‐Weight Analytes

Sharma

Petri

Jonas

et al. 2014

Macro Chemistry & Physics

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New composite layer architecture of 3D hydrogel polymer network that is loaded with molecularly imprinted polymer nanoparticles (nanoMIP) is reported for direct optical detection of low-molecular-weight compounds. This composite layer is attached to the metallic surface of a surface plasmon resonance (SPR) sensor in order to simultaneously serve as an optical waveguide and large capacity binding-matrix for imprinted target analyte. Optical waveguide spectroscopy (OWS) is used as a labelfree readout method allowing direct measurement of refractive index changes that are associated with molecular binding events inside the matrix. This approach is implemented by using a photocrosslinkable poly( N -isopropylacrylamide)-based hydrogel and poly[(ethylene glycol dimethylacrylate)-(methacrylic acid)] nanoparticles that are imprinted with L -Boc-phenylalanine-anilide ( L -BFA, molecular weight 353 g mol −1 ). Titration experiments with the specifi c target and other structurally similar reference compounds show good specifi city and limit of detection for target L -BFA as low as 2 × 10 −6 M .

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“…Optical setup for Hydrogel optical waveguide spectroscopy (HOWS) [68] biosensor is depicted in Figure11. He-Ne laser with a power of 2mW at a wavelength of λ = 633nm is transmitted through a polarizer, Polarizer polarizes to transverse magnetic (TM) mode and is passed to a high refractive index (n p = 1.845) prism at [90] 0 and through a sensor chip.…”

Section: Surface Plasmon Resonance Spectroscopymentioning

confidence: 99%

Nanoplasmonics and its Applied Devices

Rahman¹

2014

Journal of Nanotechnology and Smart Materials

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Nanoplasmonics makes a connection to conventional optics to the nanoworld. Interesting performance like subwavelength focusing to invisibility cloaking, nanoplasmonics have profound applications in science and engineering world from biophotonics to nanocircuitry. Metal and dielectric have free d-shell electrons. When metal and dielectric of different refractive index come in contact, these free electrons get accumulated in a region at the metal-semiconductor interface forming nanoplasmons. Practical implementation of nano device fabrication is the most challenging task due to the dissipative losses in metal. The optimum operating condition can be achieved by the efficient use of optical gain. We review here the ongoing progress in the field of nanoplasmonic research.

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Biosensor based on hydrogel optical waveguide spectroscopy

Cited by 86 publications

References 27 publications

Biocompatible silk step-index optical waveguides

Biocompatible silk step-index optical waveguides

Molecularly Imprinted Polymer Waveguides for Direct Optical Detection of Low‐Molecular‐Weight Analytes

Nanoplasmonics and its Applied Devices

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