Biosensor based on a silicon nanowire field-effect transistor functionalized by gold nanoparticles for the highly sensitive determination of prostate specific antigen

Presnova, G. V.; Presnov, D. E.; Krupenin, V. A.; Grigorenko, G.M.; Trifonov, A. S.; Андреева, И. П.; Ignatenko, O.V.; Egorov, A. M.; Rubtsova, M. Yu.

doi:10.1016/j.bios.2016.08.054

Cited by 115 publications

(63 citation statements)

References 35 publications

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“…The SEM observation method is an effective way to evaluate the quality of the functionalization by calculating the number of nanoparticles combined on the Si-NR. 23 The results shown in Figure 3B-D demonstrated this analysis. This is a direct evidence of the successful functionalization on the fabricated device.…”

supporting

confidence: 55%

“…The device has increasingly attracted worldwide attention with its potential clinical applications. [19][20][21][22][23] correspondence: Tong Wang Department of endoscopy surgery, Wuxi People's Hospital Affiliated to Nanjing Medical University, 299 Qingyang road, Wuxi 214023, People's republic of china Tel +86 510 8270 0778 email aanti@163.com…”

Section: Introductionmentioning

confidence: 99%

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Top-down nanofabrication of silicon nanoribbon field effect transistor (Si-NR FET) for carcinoembryonic antigen detection

Bao¹,

Sun²,

Zhao³

et al. 2017

IJN

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Sensitive and quantitative detection of tumor markers is highly required in the clinic for cancer diagnosis and consequent treatment. A field-effect transistor-based (FET-based) nanobiosensor emerges with characteristics of being label-free, real-time, having high sensitivity, and providing direct electrical readout for detection of biomarkers. In this paper, a top–down approach is proposed and implemented to fulfill a novel silicon nano-ribbon FET, which acts as biomarker sensor for future clinical application. Compared with the bottom–up approach, a top–down fabrication approach can confine width and length of the silicon FET precisely to control its electrical properties. The silicon nanoribbon (Si-NR) transistor is fabricated on a Silicon-on-Insulator (SOI) substrate by a top–down approach with complementary metal oxide semiconductor (CMOS)-compatible technology. After the preparation, the surface of Si-NR is functionalized with 3-aminopropyltriethoxysilane (APTES). Glutaraldehyde is utilized to bind the amino terminals of APTES and antibody on the surface. Finally, a microfluidic channel is integrated on the top of the device, acting as a flowing channel for the carcinoembryonic antigen (CEA) solution. The Si-NR FET is 120 nm in width and 25 nm in height, with ambipolar electrical characteristics. A logarithmic relationship between the changing ratio of the current and the CEA concentration is measured in the range of 0.1–100 ng/mL. The sensitivity of detection is measured as 10 pg/mL. The top–down fabricated biochip shows feasibility in direct detecting of CEA with the benefits of real-time, low cost, and high sensitivity as a promising biosensor for tumor early diagnosis.

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supporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Top-down nanofabrication of silicon nanoribbon field effect transistor (Si-NR FET) for carcinoembryonic antigen detection

Bao¹,

Sun²,

Zhao³

et al. 2017

IJN

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“…Since gold is a highly conductive element, it is expected that a sample containing more of it would have an enhanced conductivity. Previous studies developing a transistor have found that gold nanoparticles resulted in improved electrical performances [15]. The sample sputtered for 8 min with gold resulted in a higher conductivity than that sputtered for only 4 min.…”

Section: Resultsmentioning

confidence: 99%

“…Gold nanoparticles have important properties including their conductivity, high surface-to-volume ratio, excellent molecular recognition, and high surface energy [15, 16]. Their unique chemical and physical properties help to transfer electrons from the biospecific layer to the electrode surface [15]. Gold nanoparticles also increase the sensitivity of biochemical detection of electrochemical biosensors [17, 18].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Electrical Conductive Silica Nanofiber/Gold Nanoparticle Composite by Laser Pulses and Sputtering Technique

2017

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Biocompatible-sensing materials hold an important role in biomedical applications where there is a need to translate biological responses into electrical signals. Increasing the biocompatibility of these sensing devices generally causes a reduction in the overall conductivity due to the processing techniques. Silicon is becoming a more feasible and available option for use in these applications due to its semiconductor properties and availability. When processed to be porous, it has shown promising biocompatibility; however, a reduction in its conductivity is caused by its oxidization. To overcome this, gold embedding through sputtering techniques are proposed in this research as a means of controlling and further imparting electrical properties to laser induced silicon oxide nanofibers. Single crystalline silicon wafers were laser processed using an Nd:YAG pulsed nanosecond laser system at different laser parameters before undergoing gold sputtering. Controlling the scanning parameters (e.g., smaller line spacings) was found to induce the formation of nanofibrous structures, whose diameters grew with increasing overlaps (number of laser beam scanning through the same path). At larger line spacings, nano and microparticle formation was observed. Overlap (OL) increases led to higher light absorbance’s by the wafers. The gold sputtered samples resulted in greater conductivities at higher gold concentrations, especially in samples with smaller fiber sizes. Overall, these findings show promising results for the future of silicon as a semiconductor and a biocompatible material for its use and development in the improvement of sensing applications.

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“…[10,26,32,[46][47][48][49][50][51] As a first application, [43] Lieber's group modified the surface using 3-aminopropyltriethoxysilane (APTES), obtaining a double surface functionality with the amino groups from APTES and the hydroxyl groups from the silicon dioxide surface. [45] Inspired by these works, myriads of publications have exploited the broad range of possibilities in SiNW sensorics (Figure 1), from detection of biomolecules for diagnostic purposes [36,37,[52][53][54][55] to recording of cell activity, [46,[56][57][58][59][60][61] intracellular signal control, [49,62,63] tissue monitoring, [4,25] food safety, [64] and gas [65,66] and explosive sensing. They also functionalized the surface with biotin for specific detection of streptavidin, obtaining detection capabilities down to 10 × 10 −12 m. Only 3 and 4 years later, they could demonstrate large arrays of individually addressable sensors for multiplexed detection of single viruses [48] and protein markers at femtomolar concentrations.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Silicon Nanowire Devices and Their Functional Diversity

et al. 2019

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In the pool of nanostructured materials, silicon nanostructures are known as conventionally used building blocks of commercially available electronic devices. Their application areas span from miniaturized elements of devices and circuits to ultrasensitive biosensors for diagnostics. In this Review, the current trends in the developments of silicon nanowire‐based devices are summarized, and their functionalities, novel architectures, and applications are discussed from the point of view of analog electronics, arisen from the ability of (bio)chemical gating of the carrier channel. Hybrid nanowire‐based devices are introduced and described as systems decorated by, e.g., organic complexes (biomolecules, polymers, and organic films), aimed to substantially extend their functionality, compared to traditional systems. Their functional diversity is explored considering their architecture as well as areas of their applications, outlining several groups of devices that benefit from the coatings. The first group is the biosensors that are able to represent label‐free assays thanks to the attached biological receptors. The second group is represented by devices for optoelectronics that acquire higher optical sensitivity or efficiency due to the specific photosensitive decoration of the nanowires. Finally, the so‐called new bioinspired neuromorphic devices are shown, which are aimed to mimic the functions of the biological cells, e.g., neurons and synapses.

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Biosensor based on a silicon nanowire field-effect transistor functionalized by gold nanoparticles for the highly sensitive determination of prostate specific antigen

Cited by 115 publications

References 35 publications

Top-down nanofabrication of silicon nanoribbon field effect transistor (Si-NR FET) for carcinoembryonic antigen detection

Top-down nanofabrication of silicon nanoribbon field effect transistor (Si-NR FET) for carcinoembryonic antigen detection

Synthesis of Electrical Conductive Silica Nanofiber/Gold Nanoparticle Composite by Laser Pulses and Sputtering Technique

Hybrid Silicon Nanowire Devices and Their Functional Diversity

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