Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation

Gaillard, Marine; Thuaire, Aurélie; Nonglaton, Guillaume; Agache, Vincent; Roupioz, Yoann; Raillon, Camille

doi:10.1039/c9an01949a

Cited by 18 publications

(20 citation statements)

References 122 publications

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“…The use of peptidic ligands to capture EVs is a promising approach which could complement the traditional use of other affinity probes such as antibodies [40]. Peptides are indeed attractive due to low costs, stability, synthetic versatility and ease of tunability of functional groups.…”

Section: Discussionmentioning

confidence: 99%

Membrane‐binding peptides for extracellular vesicles on‐chip analysis

Gori¹,

Romanato²,

Bergamaschi³

et al. 2020

J of Extracellular Vesicle

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Small extracellular vesicles (sEVs) present fairly distinctive lipid membrane features in the extracellular environment. These include high curvature, lipid-packing defects and a relative abundance in lipids such as phosphatidylserine and ceramide. sEV membrane could be then considered as a "universal" marker, alternative or complementary to traditional, characteristic, surface-associated proteins. Here, we introduce the use of membrane-sensing peptides as new, highly efficient ligands to directly integrate sEV capturing and analysis on a microarray platform. Samples were analysed by label-free, singleparticle counting and sizing, and by fluorescence co-localisation immune staining with fluorescent anti-CD9/anti-CD63/anti-CD81 antibodies. Peptides performed as selective yet general sEV baits and showed a binding capacity higher than anti-tetraspanins antibodies. Insights into surface chemistry for optimal peptide performances are also discussed, as capturing efficiency is strictly bound to probes surface orientation effects. We anticipate that this new class of ligands, also due to the versatility and limited costs of synthetic peptides, may greatly enrich the molecular toolbox for EV analysis.

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Section: Discussionmentioning

confidence: 99%

Membrane‐binding peptides for extracellular vesicles on‐chip analysis

Gori¹,

Romanato²,

Bergamaschi³

et al. 2020

J of Extracellular Vesicle

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“…Recently, considering the importance of exosomes in the liquid biopsy of cancer, many studies have explored new detection techniques over the past few decades. 16 A series of novel technologies have been developed for the determination of exosomes, including uorescence detection, 17 electrochemical biosensors, 5,14,18,19 immunosensors, 13,20 surface plasmon resonance (SPR), [21][22][23] microuidic devices, 21 electrochemiluminescence, 24 aptasensors, 25 and spectroscopic analysis. 26 However, these established exosome assays usually involve labour-intensive steps and require extended quantitative protocols and expensive laboratory infrastructures.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

et al. 2021

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“…In the current state, their use is mostly limited for isolation and detection of EVs of mammalian origin, and mostly in body fluids or cell culture supernatants. Discovery of specific target molecules on the surface of bacterial EVs would pave the way for their isolation and detection by use of similar technologies that are recently reviewed by Gaillard et al [233].…”

Section: Use Of Biosensors For Detection Of Foodborne Pathogens Evsmentioning

confidence: 99%

“…Surface layers of magnetic particles can be modified to obtain affinity to a specific target molecule. Surface modification can be achieved by immobilization of affinity ligands, for example, antibodies or DNA aptamers [233]. Because of the magnetic property of such particles, they can be handily separated from complex samples via magnetic solid‐phase extraction (MSPE).…”

Section: New Trends In Detection Of Foodborne Pathogensmentioning

confidence: 99%

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Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Begić

Josić

2020

Electrophoresis

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Biofilm formation and extracellular microvesicles-The way of foodborne pathogens toward resistance Almost all known foodborne pathogens are able to form biofilms as one of the strategies for survival under harsh living conditions, to ward off the inhibition and the disinfection during food production, transport and storage, as well as during cleaning and sanitation of corresponding facilities. Biofilms are communities where microbial cells live under constant intracellular interaction and communication. Members of the biofilm community are embedded into extracellular matrix that contains polysaccharides, DNA, lipids, proteins, and small molecules that protect microorganisms and enable their intercellular communication under stress conditions. Membrane vesicles (MVs) are produced by both Gram positive and Gram negative bacteria. These lipid membrane-enveloped nanoparticles play an important role in biofilm genesis and in communication between different biofilm members. Furthermore, MVs are involved in other important steps of bacterial life like cell wall modeling, cellular division, and intercellular communication. They also carry toxins and virulence factors, as well as nucleic acids and different metabolites, and play a key role in host infections. After entering host cells, MVs can start many pathologic processes and cause serious harm and cell death. Prevention and inhibition of both biofilm formation and shedding of MVs by foodborne pathogens has a very important role in food production, storage, and food safety in general. Better knowledge of biofilm formation and maintaining, as well as the role of microbial vesicles in this process and in the process of host cells' infection is essential for food safety and prevention of both food spoilage and host infection.

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Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation

Abstract: This Minireview focuses on the contribution of biomolecules used as ligands in affinity-based biosensors for the detection and isolation of Extracellular Vesicles (EVs).

Cited by 18 publications

References 122 publications

Membrane‐binding peptides for extracellular vesicles on‐chip analysis

Membrane‐binding peptides for extracellular vesicles on‐chip analysis

Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

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