Biosensing and monitoring of cell populations using the hydrogel bacterial microchip

Fesenko, D. O.; Nasedkina, T. V.; Prokopenko, Dmitry; Mirzabekov, Andrei D.

doi:10.1016/j.bios.2004.06.005

Cited by 32 publications

(16 citation statements)

References 12 publications

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“…(124) Many researchers have applied cell array biosensors for environmental toxic analysis (125) and bacterial metabolism monitoring. (126) Lee et al (127) presented the application of a cell array chip that was fabricated using 12 different recombinant bioluminescent bacteria to analyze oxidative stress in a high-throughput manner, and each strain included was responsive to one or more of the compounds tested.…”

Section: Developing Portable Biosensors For On-site Monitoringmentioning

confidence: 99%

Microbial Biosensors for Environmental Monitoring and Food Analysis

Ying

2011

Food Reviews International

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A microbial biosensor is an elaborate analytic device that uses microorganisms as recognition elements, mainly for application in environmental monitoring, food safety, military defense, and medicine. The selection and immobilization of microorganisms are key steps that must first be addressed for microbial biosensors. Currently, genetically modified microorganisms play an increasingly significant role in improving the capacity of biosensors. Electrochemical and optical types of transducers have been widely employed in microbial biosensors, although bioluminescence and fluorescence methods have been highlighted recently. Additionally, the microbial fuel cell (MFC), which has been mainly applied in biological oxygen demand (BOD) biosensors, is a promising technology. This article reviews recent developments of microbial biosensors with respect to their applications in environmental monitoring and food analysis, including measurement of a variety of common pollutants, products in fermenting processes, antibiotic residues, and toxins in food. Current limitations and prospective future directions, such as performance optimization, developing portable biosensors for on-site monitoring, combination of genetic and DNA approaches, nanotechnology, and phage-based biosensors for foodborne pathogens, are also discussed in this review.

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Section: Developing Portable Biosensors For On-site Monitoringmentioning

confidence: 99%

Microbial Biosensors for Environmental Monitoring and Food Analysis

Ying

2011

Food Reviews International

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“…Useful tools for this purpose are array robots of various kinds. For example, using an Affymetrix 417 arrayer robot (Affymetrix, Santa Clara, CA), Fesenko et al [95] have fabricated an acrylamide-based hydrogel bacterial microchip (HBMChip). The microchip consisted of an array of hemispherical gel elements, 0.3-60 nL in volume, attached to a hydrophobic glass surface and containing microbial cells.…”

Section: Cell Array Deposition Techniquesmentioning

confidence: 99%

“…Reported solutions include freeze/vacuum drying [106][107][108] as well as cell encapsulation and entrapment in a large variety of polymers. Methods used for the fabrication of bacterial cell arrays (mostly of genetically engineered bioluminescent reporters) included agar [72,73], agarose [90], alginate [109,110], carrageenan [111], collagen [112], latex [96], and polyacrylamide [95]. The latter has been reported to cause loss of viability [109,113].…”

Section: Cell Immobilization Maintenance and Long-term Storagementioning

confidence: 99%

Microbial Cell Arrays

Elad

Lee

Gu³

et al. 2009

Whole Cell Sensing Systems I

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The coming of age of whole-cell biosensors, combined with the continuing advances in array technologies, has prepared the ground for the next step in the evolution of both disciplines - the whole cell array. In the present chapter, we highlight the state-of-the-art in the different disciplines essential for a functional bacterial array. These include the genetic engineering of the biological components, their immobilization in different polymers, technologies for live cell deposition and patterning on different types of solid surfaces, and cellular viability maintenance. Also reviewed are the types of signals emitted by the reporter cell arrays, some of the transduction methodologies for reading these signals, and the mathematical approaches proposed for their analysis. Finally, we review some of the potential applications for bacterial cell arrays, and list the future needs for their maturation: a richer arsenal of high-performance reporter strains, better methodologies for their incorporation into hardware platforms, design of appropriate detection circuits, the continuing development of dedicated algorithms for multiplex signal analysis, and - most importantly - enhanced long term maintenance of viability and activity on the fabricated biochips.

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“…Various techniques are used to achieve an optimal cell hosting character among the substrates and scaffolds. [3][4][5] A few hours after cells are seeded into a sterile culture dish containing a nutrient-rich cell culturing medium they adhere to the surface and start to proliferate. Depending on the cell type cells may differentiate into a mature and/or activated form, as exemplified by neurons, see Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Organic Bioelectronics

Berggren¹,

2007

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During the last two decades, organic electroactive materials have been explored as the working material in a vast array of electronic devices, promising low‐cost, flexible, and easily manufactured systems. The same materials also possess features that make them unique in bioelectronics applications, where electronic signals are translated into biosignals and vice versa. Here we review, in the broadest sense, the field of organic bioelectronics, describing the electronic properties and mechanisms of the organic electronic materials that are utilized in specific biological experiments.

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Biosensing and monitoring of cell populations using the hydrogel bacterial microchip

Cited by 32 publications

References 12 publications

Microbial Biosensors for Environmental Monitoring and Food Analysis

Microbial Biosensors for Environmental Monitoring and Food Analysis

Microbial Cell Arrays

Organic Bioelectronics

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