Bioscouring of Cotton using Lipase from Marine Bacteria Bacillus sonorensis

Nerurkar, Madhura; Joshi, Manasi; Adivarekar, Ravindra V.

doi:10.1007/s12010-014-1259-6

Cited by 27 publications

(7 citation statements)

References 6 publications

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“…Hence, the strain 4R was identified as Bacillus sonorensis . So far, Bacillus sonorensis was reported to be isolated from Sonoran Desert [ 15 ] and Kalbadevi estuary, Mumbai [ 16 ]. Abundance of thermophilic Bacillus species including Bacillus licheniformis , Bacillus aerius , Bacillus sonorensis , Bacillus subtilis , and Bacillus amyloliquefaciens in hot springs, salt marshes, and desert soil of Morocco has been observed by Aanniz et al [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Hyperthermostable Alkaline Lipase fromBacillus sonorensis4R

2016

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Hyperthermostable alkaline lipase from Bacillus sonorensis 4R was purified and characterized. The enzyme production was carried out at 80°C and 9.0 pH in glucose-tween inorganic salt broth under static conditions for 96 h. Lipase was purified by anion exchange chromatography by 12.15 fold with a yield of 1.98%. The molecular weight of lipase was found to be 21.87 KDa by SDS-PAGE. The enzyme activity was optimal at 80°C with t 1/2 of 150 min and at 90°C, 100°C, 110°C, and 120°C; the respective values were 121.59 min, 90.01 min, 70.01 min, and 50 min. The enzyme was highly activated by Mg and t 1/2 values at 80°C were increased from 150 min to 180 min when magnesium and mannitol were added in combination. The activation energy calculated from Arrhenius plot was 31.102 KJ/mol. At 80–120°C, values of ΔH and ΔG were in the range of 28.16–27.83 KJ/mol and 102.79 KJ/mol to 111.66 KJ/mol, respectively. Lipase activity was highest at 9.0 pH and stable for 2 hours at this pH at 80°C. Pretreatment of lipase with MgSO4 and CaSO4 stimulated enzyme activity by 249.94% and 30.2%, respectively. The enzyme activity was greatly reduced by CoCl2, CdCl2, HgCl2, CuCl2, Pb(NO3)2, PMSF, orlistat, oleic acid, iodine, EDTA, and urea.

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Section: Resultsmentioning

confidence: 99%

Characterization of a Hyperthermostable Alkaline Lipase fromBacillus sonorensis4R

2016

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“…Majority of surfactants are of bacterial origin reported as B. 41 reported isolation of lipase from marine bacteria Bacillus sonorensis for bioscouring of impurities from cotton fabric. In the present study, the highest lipase activity of 58.72 and 56.45 U ml -1 was attained using olive oil (2%) and tributyrin (2%) respectively.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Crude Oil Using Marine <i>Bacillus species</i> from Vadinar Coast, Gujarat, India

Mulani¹,

Fulke²,

DeSouza³

et al. 2017

Current Science

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The marine environment is open to large sources of toxic organic waste in the form of accidental oil spills. Therefore, it is important to study microbial degradation processes that help reduce the damage caused to the environment. Universally, oil spills produce enormous public anxiety and highlight the need for costeffective, indigenous and environmentally acceptable bioremediation technologies. In recent times, advanced remedial techniques have been opted, such as solidifying, skimming, controlled burning and bioremediation. The present study aimed to isolate crude oil-degrading marine bacteria from Vadinar coastal area of Gujarat, India. Among seven isolates, three potential bacterial strains were chosen for crude oil and petroleum hydrocarbon (PHC) degradation, which were analysed by UV spectrophotometric and fluorometric analysis. These bacterial cultures were verified by 16S rRNA gene sequencing and identified as Bacillus species. Phylogenetic analysis was carried out to confirm the evolutionary relationship with existing oil-degrading species. In the present study, drop collapse, oil spreading and emulsification assay were performed to detect biosurfactant production. Bacillus sp. NM1 KT354277 was capable of degrading 50% of PHCs at the end 72 h for one week under rotary incubation in ONR7a medium. Among the studied strains, Bacillus sp. NM3 KT354278 showed promising lipase activity, viz. 60.72 and 61.19 U ml -1 for 2% of olive oil and tributyrin respectively. Thus, the present study explores indigenous marine isolates that could be utilized as a potential alternative for oilspill remediation in future.

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“…One hundred microlitres of the purification bacterial solution obtained in the above test was evenly spread on the lipase test medium. After that, the inoculated plates were incubated at 37°C for 24 h in constant temperature cultivation and checked for the appearance of hydrolysis circles around the colonies (Nerurkar et al, 2015). The lipase production was determined by the appearance of hydrolysis circles and scored as follows: +, representing able to produce enzymes, means positive: −, representing unable to produce the enzyme, means negative.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of probiotic potential and fermentation characteristics of Bacillus amyloliquefaciens LSG2‐8 from intestine of Rhynchocypris lagowskii

Zhang

et al. 2021

Aquaculture Research

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In this study, 30 candidate bacterial strains were isolated from the intestine of Rhynchocypris lagowskii. LSG2‐8 was screened out through enzyme production test, antibiotic susceptibility test and anti‐pathogens ability. The LSG2‐8 was identified as Bacillus amyloliquefaciens. Bio‐safety assay confirmed that the LSG2‐8 was not pathogenic to the host fish and performed better in the tolerance test. The results of the adherence ability showed that the adhesion rate of LSG2‐8 was 36% in vitro intestinal mucosal adhesion model. Analysis of their surface‐adhesive proteins revealed that LSG2‐8 bacterial cell surface lectin mainly exhibited glycoprotein properties. The results of the interaction test on temperature, initial pH and inoculation amount showed that the optimum fermentation conditions for the LSG2‐8 were 30°C, 6.0 and 4%, respectively. In summary, the B. amyloliquefaciens LSG2‐8 from the intestine of R. lagowskii is a promising candidate strain for feed fermentation.

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Bioscouring of Cotton using Lipase from Marine Bacteria Bacillus sonorensis

Cited by 27 publications

References 6 publications

Characterization of a Hyperthermostable Alkaline Lipase fromBacillus sonorensis4R

Characterization of a Hyperthermostable Alkaline Lipase fromBacillus sonorensis4R

Biodegradation of Crude Oil Using Marine <i>Bacillus species</i> from Vadinar Coast, Gujarat, India

Evaluation of probiotic potential and fermentation characteristics of Bacillus amyloliquefaciens LSG2‐8 from intestine of Rhynchocypris lagowskii

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