Bioremediation potential of native hydrocarbon degrading bacterial strains in crude oil contaminated soil under microcosm study

Roy, Abhijit Sarma; Baruah, R. N.; Borah, Maina; Singh, Anil Kumar; Boruah, Hari Prasanna Deka; Saikia, N.; Deka, Manab; Dutta, Nipu; Bora, Tarun C.

doi:10.1016/j.ibiod.2014.03.024

Cited by 150 publications

(66 citation statements)

References 42 publications

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“…Overall, the percentages of root and shoot length inhibition before treatment were significantly higher with or without glucose (as an additional carbon source), while after treatment of oil-based paint the percentages of root and shoot lengths were reduced. Roy et al [37] confirmed the improvement of soil quality by testing on rice (Oryza sativa) and mung (Vigna radiata), and conducting an earthworm mortality bioassay in its microcosm study. They have concluded that the combined use of nutrient supplements along with crude oil (hydrocarbon)-degrading bacteria could revive crude oil-contaminated soil effectively on a large scale.…”

Section: Phytotoxic Effectmentioning

confidence: 98%

Bioremediation of Oil-Based Paint from Aqueous Media by Novel Indigenous Brevibacillus parabrevis Strain NAP3 and its Toxicity Assessment

Phulpoto

Qazi

Mangi

et al. 2017

Pol. J. Environ. Stud.

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The present study sought to explore the bioremediation of oil-based paint from aqueous media using indigenous bacterial isolate Brevibacillus parabrevis strain NAP3 and its toxicity assessment against prokaryotic and eukaryotic biological models. The bacterial isolate was initially screened for bioremediation potential in mineral salts medium containing oil-based paint (conc. 300 ppm, w/v) under shake flask settings. Moreover, the percentage removal of oil-based paint from aqueous media was investigated using a spectrophotometer at 285 nm under two different experimental conditions, i.e., with and without glucose. Evidently, the bacterial isolate displayed maximum oil-based paint removal of 83% in flasks containing glucose as an additional carbon source after 14 days of the treatment, whereas without glucose supplementation it reached up to 78%. The Fourier transform infrared (FTIR) spectra showed noticeable biodegradative changes of the oil-based paint relevant to peaks in the wave number frequency range of 800-600 cm , and 3,200 to 3,400 cm -1 (corresponding to C-H, C-O-N, C-O-C, and CH 2 aliphatic stretch and terminal CH 3 bonds, and N-H bonds, respectively, in treated samples as compared to controls; non-inoculated samples). After treatment, the cellfree supernatants containing oil-based paints displayed reduced cytotoxicity against brine shrimp larvae, phytotoxicity against wild reddish seeds, and antimicrobial activity against selected pathogenic bacteria and fungi, which indicated the possible use of B. parabrevis as potential oil-based paint-degrading bacterium in order to restore paint-polluted environments.

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Section: Phytotoxic Effectmentioning

confidence: 98%

Bioremediation of Oil-Based Paint from Aqueous Media by Novel Indigenous Brevibacillus parabrevis Strain NAP3 and its Toxicity Assessment

Phulpoto

Qazi

Mangi

et al. 2017

Pol. J. Environ. Stud.

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“…최근 쿠웨이트 정화사업 등에 의해 원유오염토양 처리 에 대한 관심이 높아지고 있지만 (Gao et al, 2014;Roy et al, 2014) 아직 원유분해미생물을 이용한 토양 정화에 관한 국내 연구는 미진한 상황이다 (Choi et al, 2014). TPH 농도는 1,890 ± 51 mg/kg까지 저감되어 국내 "3"지 역 정화목표(TPH 2,000 mg/kg 미만)를 달성하였다( Table 3).…”

Section: 서 론unclassified

Evaluation of Bioremediation Efficiency of Crude Oil Degrading Microorganisms Depending on Temperature

Kim¹,

Lee²,

Jeong³

et al. 2016

Journal of Soil and Groundwater Environment

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Bioremediation is one of the most effective ways to remediate TPH-contaminated sites. However, under actual field conditions that are not at the optimum temperature, degradation of microorganisms is generally reduced, which is why the efficiency of biodegradation is known to be significantly affected by the soil temperature. Therefore, in this study, the labscale experiment was conducted using indigenous crude oil degrading microorganisms isolated from crude oil contaminated site to evaluate the remediation efficiency. Crude oil degrading microorganisms were isolated from crude oil contaminated soil and temperature, which is a significant factor affecting the remediation efficiency of land farming, was adjusted to evaluate the microbial crude oil degrading ability, degradation time, and remediation efficiency. In order to assess the field applicability, the remediation efficiency was evaluated using crude oil contaminated soil (average TPH concentration of 10,000 mg/kg or more) from the OO premises. Followed by the application of microorganisms at 30 o C, the bioremediation process reduced its initial TPH concentration of 10,812 mg/kg down to 1,890 mg/kg in 56 days, which was about an 83% remediation efficiency. By analyzing the correlation among the total number of cells, the number of effective cells, and TPH concentration, it was found that the number of effective microorganisms drastically increased during the period from 10 to 20 days while there was a sharp decrease in TPH concentration. Therefore, we confirmed the applicability of land farming with isolated microorganisms consortium to crude oil contaminated site, which is also expected to be applicable to bioremediation of other recalcitrant materials.

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“…Another study reported that degradation of 4.9% to 29.6% of crude oil occurred within 15 days (Okoh, 2003). In another report, around 60% of petroleum hydrocarbons degraded using a mixture of isolated oil degraders and nutrient supplements after 30 days (Roy, 2014) and yet in another study, hydrocarbon removal ability of isolated bacterial strains from oil polluted site was 83.49% (C8-C35) in a 75 days period when the crude oil concentration was 2% (v/v) (Varjani, 2015); while we succeed to have isolates degrade 93% of petroleum hydrocarbons through sequential adaptation and without adding any nutritional supplement or surfactant after 28 days.…”

Section: Biodegradation Ability Of Bacteria Before and After Adaptationmentioning

confidence: 99%

Sequential Preconditioning and Its Effect on Crude Oil Bioremediation by Indigenous Soil Bacteria

Karimi¹,

Halimoon²,

Zakaria³

2015

IJSRES

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Abstract. Preconditioning has been proved to result in a significant increase in adaptability of biological organisms to adverse environmental stimulations and various stressful conditions. Accordingly, in a new approach attempt was made to enhance hydrocarbon degradation ability of selected potential isolates from a petroleum polluted soil through sequential adaptation. Firstly 8 isolates selected based on their growth at 5% (v/v) crude oil. Second selection performed based on their ability to adapt gradually them to 10%, 20% and 50% (v/v) crude oil concentration respectively, at simulated natural conditions (6.5 pH, 32°C). Isolates transferred to higher concentration of crude oil every 14 days. Their exponential growth rate served as an indicator of their adaptation success. Selected bacteria identified biochemically and morphologically according to Bergey's manual accompanied by 16s rRNA sequencing. Bacterial species were identified as P. putida, A. lwoffi, A. hydrophila, P. stutzeri, and A. johnsonii. Bacterial isolates were tested for their biodegradation ability individually and as a consortium; based on gas chromatographic analysis the consortium proved to be more efficient in n-alkane degradation as 93% of C8-C33 was removed in liquid cultures and 70% of hydrocarbons in artificially polluted soils, in 28 days. In addition ratios of n 17 / pristane and n 18 / phytane degradation supported the promising bioremediation ability of the consortium. Comparison between adapted and un-adapted bacteria revealed that aliphatic degradation enhanced up to 40% in soil. In general the results suggest that preconditioning bacterial isolates to higher oil concentration can significantly enhance their biodegradation ability especially as a consortium.

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Bioremediation potential of native hydrocarbon degrading bacterial strains in crude oil contaminated soil under microcosm study

Cited by 150 publications

References 42 publications

Bioremediation of Oil-Based Paint from Aqueous Media by Novel Indigenous Brevibacillus parabrevis Strain NAP3 and its Toxicity Assessment

Bioremediation of Oil-Based Paint from Aqueous Media by Novel Indigenous Brevibacillus parabrevis Strain NAP3 and its Toxicity Assessment

Evaluation of Bioremediation Efficiency of Crude Oil Degrading Microorganisms Depending on Temperature

Sequential Preconditioning and Its Effect on Crude Oil Bioremediation by Indigenous Soil Bacteria

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