Bioremediation of Xenobiotic Pesticides by Bacterial Species Isolated from Flower Farm Soil around Lake Naivasha, Kenya

Anode, Saphan Ochieng; Magoma, Gabriel; Onguso, Justus M.; Abraha, Tesfamichael

doi:10.4172/2155-6199.1000450

Cited by 4 publications

(3 citation statements)

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“…The results for morphological and biochemical characteristics such as staining; motility test and cultural characters, colony morphology, Biochemical characters for samples are shown in the Table 1 below. This result has clearly indicated that the flower farm soils around Lake Naivasha has some pesticide contamination, confirming the outcomes of some studies, and hence the presence of such bacterial species which are known to have bioremediation potential of several xenobiotic chemicals in the environment [11,12,17]. The farm soils analyzed in this study has clearly shown that they are loaded with indicator organisms which are the indication of the presence of xenobiotic pesticides commonly used in major flower farms.…”

Section: Morphological and Biochemical Identification Of Bacterial Spsupporting

confidence: 86%

Isolation and Characterization of Xenobiotic Pesticide Degrading Bacterial Species in Flower Farms Around Lake Naivasha, Kenya

2018

AEWMR

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Certain microorganisms especially bacteria and fungi are able to use xenobiotic organic compounds as their carbon and nitrogen source for metabolism. Flower farms around lake Naivasha basin uses several agrochemicals especially pesticides to control pests and improve flower production. The aim of this study was to isolate and characterize morphologically and biochemically the main bacterial species that are able to grow and tolerate the pesticide contaminated farm soils. Soil samples were collected from randomly selected five greenhouses from each five flower farms namely Crescent, Elsamere, Karuturi, Malewa and Sewage farms around Lake Naivasha basin. The collected samples were processed for bacterial isolation using the nutrient agar, mac’ Conkey agar, blood agar, Luria-Bertani and Minimum Salt Media nutrient media. The conventional methods of swabbing and streaking were used. Pure colonies of isolates organisms were identified and characterized using standard microbiological technique. Morphological, cultural and biochemical characterization of bacterial species isolated from the flower farm soil samples identified mainly Pseudomonas auriginosa, Escherichia coli, Rhodococcus erythropolis and Bacillus subtilis species. Bacterial growth in pesticide consortia was quantified by monitoring colony growth of the species in liquid culture over time. The viable cell counts were determined turbidimetrically at O.D696nm. All the isolated bacterial species were able to grow in flower farm soil contaminated with organochloride and organophosphate pesticide residues. B. subtilis recorded the highest growth at 1.77±0.07 O.D696nm in pesticide mixture consortia. There was lower growth in organochloride pesticide consortia as compared to organophosphate pesticide consortia.

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Section: Morphological and Biochemical Identification Of Bacterial Spsupporting

confidence: 86%

Isolation and Characterization of Xenobiotic Pesticide Degrading Bacterial Species in Flower Farms Around Lake Naivasha, Kenya

2018

AEWMR

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“…Examples of species isolated in prior studies include Arthromobacter sp. Enterobacter strain B-14, Alcaligenes faecalis, Bacillus pumilus, Staphylococcus sp., Streptococcus sp., Achromobactor sp., Serratia marcescens, Bacillus cereus, Bacillus subtilis, Pseudomonas desmolyticum, and Pseudomonas aeruginosa ( Singh et al., 2004 ; Anwar et al., 2009 ; Yadav et al., 2015 ; Hamsavathani et al., 2017 ; Anode et al., 2018 ). These bacterial communities have been shown to catabolize and co-metabolize TCP and CP.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of chlorpyrifos degrading bacteria isolated from contaminated dairy farm soils in Nakuru County, Kenya

Asamba

Njeru

Sifuna

et al. 2022

Heliyon

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“…Past researchers have detected OP pesticide residues in food items that include lemons, oranges, beans, tomatoes, pepper, okra, cabbage, lettuce, cucumber, onions, and strawberries (Akan, 2013;Chawla et al, 2018;Yura et al, 2021). Various studies have examined the presence of pesticide residues in soil and water samples in Kenya (Lalah et al, 2003;Musa et al, 2011;Otieno et al, 2014;Anode et al, 2018;Atego et al, 2021), but only a few have explored the presence of these residues in human food products (Macharia, 2015).…”

Section: Organophosphate Residues In Food Productsmentioning

confidence: 99%

Organophosphate Pesticide Residues in Food Products in Kenya and their Chromatographic Detection: A Systematic Review

Asamba

Wepukhulu

2023

East Afr. j. agric. biotechnol.

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Organophosphate pesticides are used worldwide to control several pests and meet food demand. These chemicals harm non-target animals and people when misused. Thus, they are a health and environmental concern. The purpose of the systematic review was to synthesise the amount, breadth, and quality of evidence from empirical studies concerning the presence, type, and quantity of OPs in food products in Kenya. A systematic review was done by following the PRISMA protocol. For the identification of studies, the following databases were used: Google Scholar, Web of Science, Scopus, and PubMed. The study focused on peer-reviewed articles published between January 2001 and August 2022. Twelve studies met the inclusion criteria for the scoping review. The main methods used in detecting and quantifying organophosphates in the studies were High-Performance Liquid Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS). Most studies (86.67%) reported significant levels of OPs in food products, exceeding the MRLs and the Acceptable Daily Intake (ADIs) set by the World Health Organization. Six of the studies (50%) reported the presence of OPs in plant products (vegetables, cereals, and fruits), while three (25%) found residues in animal products (milk and meat). The most notable OP compounds detected were chlorpyrifos, acephate, profenofos, diazinon, omethoate, and dimethoate. Most of the food samples in the reviewed studies presented contamination, making them a significant risk to human health due to bioaccumulation. Studies done in Kenya for the last 20 years continue to report high levels of organophosphate residues and their metabolites in food products, both from plant and animal origin. Although the residues are below MLRs in some samples, they can accumulate at higher levels in humans, becoming a severe health risk

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Bioremediation of Xenobiotic Pesticides by Bacterial Species Isolated from Flower Farm Soil around Lake Naivasha, Kenya

Cited by 4 publications

References 0 publications

Isolation and Characterization of Xenobiotic Pesticide Degrading Bacterial Species in Flower Farms Around Lake Naivasha, Kenya

Isolation and Characterization of Xenobiotic Pesticide Degrading Bacterial Species in Flower Farms Around Lake Naivasha, Kenya

Molecular characterization of chlorpyrifos degrading bacteria isolated from contaminated dairy farm soils in Nakuru County, Kenya

Organophosphate Pesticide Residues in Food Products in Kenya and their Chromatographic Detection: A Systematic Review

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