Bioremediation of strontium and technetium contaminated groundwater using glycerol phosphate

Cleary, Adrian; Lloyd, Jonathan R.; Newsome, Laura; Shaw, Samuel; Boothman, Christopher; Boshoff, G.; Atherton, Nick; Morris, Katherine

doi:10.1016/j.chemgeo.2019.02.004

Cited by 27 publications

(56 citation statements)

References 93 publications

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“… ,,,− Here, the addition of a single “Fe” backscatterer at 2.56 Å resolved the missing features and statistically improved the fit. This suggests that an association of hydrous TcO 2 with Fe–O octahedra associated with Fe oxyhydr(oxide) phases was possible and consistent with previous studies. ,,− …”

Section: Resultssupporting

confidence: 91%

Biogeochemical Cycling of ⁹⁹Tc in Alkaline Sediments

Williamson

Lloyd

Boothman

et al. 2021

Environ. Sci. Technol.

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99Tc will be present in significant quantities in radioactive wastes including intermediate-level waste (ILW). The internationally favored concept for disposing of higher activity radioactive wastes including ILW is via deep geological disposal in an underground engineered facility located ∼200–1000 m deep. Typically, in the deep geological disposal environment, the subsurface will be saturated, cement will be used extensively as an engineering material, and iron will be ubiquitous. This means that understanding Tc biogeochemistry in high pH, cementitious environments is important to underpin safety case development. Here, alkaline sediment microcosms (pH 10) were incubated under anoxic conditions under “no added Fe(III)” and “with added Fe(III)” conditions (added as ferrihydrite) at three Tc concentrations (10–11, 10–6, and 10–4 mol L–1). In the 10–6 mol L–1 Tc experiments with no added Fe(III), ∼35% Tc(VII) removal occurred during bioreduction. Solvent extraction of the residual solution phase indicated that ∼75% of Tc was present as Tc(IV), potentially as colloids. In both biologically active and sterile control experiments with added Fe(III), Fe(II) formed during bioreduction and >90% Tc was removed from the solution, most likely due to abiotic reduction mediated by Fe(II). X-ray absorption spectroscopy (XAS) showed that in bioreduced sediments, Tc was present as hydrous TcO2-like phases, with some evidence for an Fe association. When reduced sediments with added Fe(III) were air oxidized, there was a significant loss of Fe(II) over 1 month (∼50%), yet this was coupled to only modest Tc remobilization (∼25%). Here, XAS analysis suggested that with air oxidation, partial incorporation of Tc(IV) into newly forming Fe oxyhydr(oxide) minerals may be occurring. These data suggest that in Fe-rich, alkaline environments, biologically mediated processes may limit Tc mobility.

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Section: Resultssupporting

confidence: 91%

Biogeochemical Cycling of ⁹⁹Tc in Alkaline Sediments

Williamson

Lloyd

Boothman

et al. 2021

Environ. Sci. Technol.

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“…In addition, phosphate mineral formation by several yeast strains may involve an organic source of phosphorus such as glycerol 2-phosphate or phytic acid, in the presence of soluble U (Liang et al, 2016). Biomineralization processes with other radionuclides, such as caesium, strontium, technetium, plutonium or neptunium, have also been described (Macaskie et al, 1994;Macaskie and Basnakova, 1998;Hallberg and Ferris, 2004;Mitchell and Ferris, 2005;Thorpe et al, 2016, Cleary et al, 2019.…”

Section: Microbial Interactions With Radionuclidesmentioning

confidence: 99%

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

López-Fernández

Jroundi

Ruiz-Fresneda

et al. 2020

Microbial Biotechnology

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Radionuclides (RNs) generated by nuclear and civil industries are released in natural ecosystems and may have a hazardous impact on human health and the environment. RN-polluted environments harbour different microbial species that become highly tolerant of these elements through mechanisms including biosorption, biotransformation, biomineralization and intracellular accumulation. Such microbial-RN interaction processes hold biotechnological potential for the design of bioremediation strategies to deal with several contamination problems. This paper, with its multidisciplinary approach, provides a state-of-theart review of most research endeavours aimed to elucidate how microbes deal with radionuclides and how they tolerate ionizing radiations. In addition, the most recent findings related to new biotechnological applications of microbes in the bioremediation of radionuclides and in the long-term disposal of nuclear wastes are described and discussed.

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“…Several studies have found Pseudomonas in oil reservoirs 86 – 92 , and according to Cui et al 93 , Pseudomonas and Acinetobacter are genera that can effectively use crude oil as a carbon source, being able to survive and reproduce at the oil–water interface. Species of the genus Pseudomonas are facultative anaerobes capable of performing nitrification and nitrate reduction using various carbon substrates 94 , 95 . In a study by Braun and Gibson 96 , the authors reported on two bacterial strains of the genus Pseudomonas that were able to degrade, under anaerobic conditions, 2-aminobenzoate (anthranilic acid) to CO2 and NH 4 +.…”

Section: Resultsmentioning

confidence: 99%

Sulphate-reducing bacterial community structure from produced water of the Periquito and Galo de Campina onshore oilfields in Brazil

Tiburcio

Macrae

Peixoto

et al. 2021

Sci Rep

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Sulphate-reducing bacteria (SRB) cause fouling, souring, corrosion and produce H2S during oil and gas production. Produced water obtained from Periquito (PQO) and Galo de Campina (GC) onshore oilfields in Brazil was investigated for SRB. Produced water with Postgate B, Postgate C and Baars media was incubated anaerobically for 20 days. DNA was extracted, 16S rDNA PCR amplified and fragments were sequenced using Illumina TruSeq. 4.2 million sequence reads were analysed and deposited at NCBI SAR accession number SRP149784. No significant differences in microbial community composition could be attributed to the different media but significant differences in the SRB were observed between the two oil fields. The dominant bacterial orders detected from both oilfields were Desulfovibrionales, Pseudomonadales and Enterobacteriales. The genus Pseudomonas was found predominantly in the GC oilfield and Pleomorphominas and Shewanella were features of the PQO oilfield. 11% and 7.6% of the sequences at GC and PQO were not classified at the genus level but could be partially identified at the order level. Relative abundances changed for Desulfovibrio from 29.8% at PQO to 16.1% at GC. Clostridium varied from 2.8% at PQO and 2.4% at GC. These data provide the first description of SRB from onshore produced water in Brazil and reinforce the importance of Desulfovibrionales, Pseudomonadales, and Enterobacteriales in produced water globally. Identifying potentially harmful microbes is an important first step in developing microbial solutions that prevent their proliferation.

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Bioremediation of strontium and technetium contaminated groundwater using glycerol phosphate

Cited by 27 publications

References 93 publications

Biogeochemical Cycling of ⁹⁹Tc in Alkaline Sediments

Biogeochemical Cycling of ⁹⁹Tc in Alkaline Sediments

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Sulphate-reducing bacterial community structure from produced water of the Periquito and Galo de Campina onshore oilfields in Brazil

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Bioremediation of strontium and technetium contaminated groundwater using glycerol phosphate

Cited by 27 publications

References 93 publications

Biogeochemical Cycling of 99Tc in Alkaline Sediments

Biogeochemical Cycling of 99Tc in Alkaline Sediments

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Sulphate-reducing bacterial community structure from produced water of the Periquito and Galo de Campina onshore oilfields in Brazil

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Biogeochemical Cycling of ⁹⁹Tc in Alkaline Sediments

Biogeochemical Cycling of ⁹⁹Tc in Alkaline Sediments