Microorganisms such as Stenotrophomonas bentonitica could influence the safety of the deep geological repository system by producing nanoparticles and volatile compounds of selenium.
Radionuclides (RNs) generated by nuclear and civil industries are released in natural ecosystems and may have a hazardous impact on human health and the environment. RN-polluted environments harbour different microbial species that become highly tolerant of these elements through mechanisms including biosorption, biotransformation, biomineralization and intracellular accumulation. Such microbial-RN interaction processes hold biotechnological potential for the design of bioremediation strategies to deal with several contamination problems. This paper, with its multidisciplinary approach, provides a state-of-theart review of most research endeavours aimed to elucidate how microbes deal with radionuclides and how they tolerate ionizing radiations. In addition, the most recent findings related to new biotechnological applications of microbes in the bioremediation of radionuclides and in the long-term disposal of nuclear wastes are described and discussed.
A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas rhizophila e-p10T (99.2 % sequence similarity), followed by Stenotrophomonas pavanii ICB 89T (98.5 %), Stenotrophomonas maltophilia IAM 12423T, Stenotrophomonas chelatiphaga LPM-5T and Stenotrophomonas tumulicola T5916-2-1bT (all 98.3 %). Pairwise sequence similarities to all other type strains of species of the genus Stenotrophomonas were below 98 %. Genome-based calculations (orthologous average nucleotide identity, original average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C15 : 0 and anteiso-C15 : 0 as major components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7T. Based on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7T can be differentiated clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp. nov. is proposed with BII-R7T as the type strain (=LMG 29893T=CECT 9180T=DSM 103927T).
Microbial
communities occurring in reference materials for artificial
barriers (e.g., bentonites) in future deep geological repositories
of radioactive waste can influence the migration behavior of radionuclides
such as curium (CmIII). This study investigates the molecular
interactions between CmIII and its inactive analogue europium
(EuIII) with the indigenous bentonite bacterium Stenotrophomonas bentonitica at environmentally relevant
concentrations. Potentiometric studies showed a remarkably high concentration
of phosphates at the bacterial cell wall compared to other bacteria,
revealing the great potential of S. bentonitica for
metal binding. Infrared spectroscopy (ATR-FTIR) and X-ray photoelectron
spectroscopy (XPS) confirmed the role of phosphates and carboxylate
groups from the cell envelope in the bioassociation of EuIII. Additionally, time-resolved laser-induced fluorescence spectroscopy
(TRLFS) identified phosphoryl and carboxyl groups from bacterial envelopes,
among other released complexing agents, to be involved in the EuIII and CmIII coordination. The ability of this
bacterium to form a biofilm at the surface of bentonites allows them
to immobilize trivalent lanthanide and actinides in the environment.
Elemental selenium (Se 0 ) nanomaterials undergo allotropic transition from thermodynamically-unstable to more stable phases. This process is significantly different when Se 0 nanoparticles (NPs) are produced via physico-chemical and biological pathways. While the allotropic transition of physico-chemically synthesized Se 0 is fast (minutes to hours), the biogenic Se 0 takes months to complete. The biopolymer layer covering biogenic Se 0 NPs might be the main factor controlling this retardation, but this still remains an open question. Phylogenetically-diverse bacteria reduce selenium oxyanions to red amorphous Se 0 allotrope, which has low market value. Then, red Se 0 undergoes allotropic transition to trigonal (metallic grey) allotrope, the end product having important industrial applications (e.g. semiconductors, alloys). Is it not yet clear whether biogenic Se 0 presents any biological function, or it is mainly a detoxification and respiratory by-product. The better understanding of this transition would benefit the recovery of Se 0 NPs from secondary resources and its targeted utilization with respect to each allotropic stage. This review article presents and critically discusses the main physico-chemical methods and biosynthetic pathways of Se 0 (bio)mineralization. In addition, the article proposes a conceptual model for the resource recovery potential of trigonal selenium nanomaterials in the context of circular economy.
The environmental conditions for the planned geological disposal of radioactive waste —including hyper-alkaline pH, radiation or anoxia—are expected to be extremely harsh for microbial activity. However, it is thought that microbial communities will develop in these repositories, and this would have implications for geodisposal integrity and the control of radionuclide migration through the surrounding environment. Nuclear waste contains radioactive isotopes of selenium (Se) such as 79Se, which has been identified as one of the main radionuclides in a geodisposal system. Here, we use the bacterial species Stenotrophomonas bentonitica, isolated from bentonites serving as an artificial barrier reference material in repositories, to study the reduction of selenite (SeIV) under simulated geodisposal conditions. This bacterium is able to reduce toxic SeIV anaerobically from a neutral to alkaline initial pH (up to pH 10), thereby producing elemental selenium (Se0) nanospheres and nanowires. A transformation process from amorphous Se (a-Se) nanospheres to trigonal Se (t-Se) nanowires, through the formation of monoclinic Se (m-Se) aggregates as an intermediate step, is proposed. The lesser solubility of Se0 and t-Se makes S. bentonitica a potential candidate to positively influence the security of a geodisposal system, most probably with lower efficiency rates than those obtained aerobically.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.