. 1997. Natural attenuation of diesel fuel in heavy clay soil. Can. J. Soil. Sci. 77: 469-477. Petroleum hydrocarbons in the extractable fraction decreased rapidly in a heavy clay soil contaminated with diesel fuel at 5000 mg kg -1 demonstrating natural attenuation. Natural attenuation rates for untilled soil were estimated using two distinct extraction and analysis methods, the Oil & Grease Content (O&G) (US EPA 418.1; ASTM D 95) and extractable organics (EO) (EPA 3520B; EPA 8000A). The time to 50% decrease (t 1/2 ) in the fraction varied with extraction method, Oil & Grease Content (11 d) and Extractable Organics (26 d). Complete attenuation appeared to have occurred after 74 d as Extractable Organic values for soil extracts were below the detection limit (5 µg g -1 ). An enzyme-linked immunosorbant assay (ELISA) was also used to monitor the hydrocarbons present in the soil. This also effectively demonstrated the near-complete disappearance of the hydrocarbons but was not quantitative. Soil microbial biomass carbon (chloroform fumigation-extraction) and surface CO 2 flux measurements did not show any significant impact of diesel contamination or tillage in the active microbial population. This observation serves to demonstrate the applicability of natural attenuation as a remedial strategy for heavy clay soils contaminated with diesel fuel of the type used in this study.