Bioremediation of Marine Sediments Impacted by Petroleum

Silva, Aike C. da; Oliveira, Fernando J. S.; Bernardes, Diogo Santos Viotti; França, Francisca Pessôa de

doi:10.1007/s12010-008-8457-z

Cited by 26 publications

(11 citation statements)

References 14 publications

(2 reference statements)

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“…In particular, at hydrocarbon-contaminated sites, adjustment of the C/N/P ratio is important for efficient bioremediation because of the excess amount of carbon [31]. In this context, application of red clay is a novel strategy for enhancing bioremediation of hydrocarbon-contaminated sites because red clay contains only inorganic mineral compounds such as SiO 2 , Al 2 O 3 , and Fe 2 O 3 [13].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

et al. 2015

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Red clay was previously used to enhance bioremediation of diesel-contaminated soil. It was speculated that the enhanced degradation of diesel was due to increased bacterial growth. In this study, we selected Acinetobacter oleivorans DR1, a soil-borne degrader of diesel and alkanes, as a model bacterium and performed transcriptional analysis using RNA sequencing to investigate the cellular response during hexadecane utilization and the mechanism by which red clay promotes hexadecane degradation. We confirmed that red clay promotes the growth of A. oleivorans DR1 on hexadecane, a major component of diesel, as a sole carbon source. Addition of red clay to hexadecane-utilizing DR1 cells highly upregulated β-oxidation, while genes related to alkane oxidation were highly expressed with and without red clay. Red clay also upregulated genes related to oxidative stress defense, such as superoxide dismutase, catalase, and glutaredoxin genes, suggesting that red clay supports the response of DR1 cells to oxidative stress generated during hexadecane utilization. Increased membrane fluidity in the presence of red clay was confirmed by fatty acid methyl ester analysis at different growth phases, suggesting that enhanced growth on hexadecane could be due to increased uptake of hexadecane coupled with upregulation of downstream metabolism and oxidative stress defense. The monitoring of the bacterial community in soil with red clay for a year revealed that red clay stabilized the community structure.

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

et al. 2015

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“…Recently, this statistical technique has been successfully applied in many fields [22,[32][33][34]. The statistical experiment designs most widely used in optimization experiments are termed response surface designs [22,[32][33][34][35][36][37]. These designs provide information about direct effects, pair wise interaction effects and curvilinear variable effects.…”

Section: Introductionmentioning

confidence: 99%

Factorial Designs Application to Study Enhanced Bioremediation of Soil Artificially Contaminated with Weathered Bonny Light Crude Oil through Biostimulation and Bioaugmentation Strategy

Agarry¹,

Ogunleye²

2012

JEP

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The objective of this study was designed to evaluate the effects of biostimulation and bioaugmentation amendment agents (NPK fertilizer, Tween 80 and mixed culture) on the bioremediation of tropical soil samples artificially contaminated with Weathered Bonny Light Crude Oil (WBLCO). Response Surface Methodology (RSM) with Box Behnken Design (BBD) was used with three levels and three factors of NPK fertilizer (2 -6 g), Tween 80 (5 -15 mg/l) and mixed culture (0.5 -1.5 g/l) as independent variables and WBLCO removal as dependent variable (response) in a six weeks remediation period. The results showed that the rate of WBLCO removal generally increased with increase in the amount of NPK fertilizer, Tween 80 and mixed culture (biomass), respectively. A statistically significant (P < 0.0001) second-order quadratic regression model for WBLCO removal (using design-expert statistical program (v. 6.0.8)) with a coefficient of determination, R (=0.9996) was obtained. Numerical optimization technique based on desirability function was carried out to optimize the bioremediation process. The optimum values for biostimulation and bioaugmentation amendment agents to achieve a predicted maximum WBLCO removal of 84.88 percent were found to be: NPK fertilizer, 4.25 g; Tween 80, 10.22 mg/l and mixed culture, 0.46 g/l. At this optimum point, the observed WBLCO removal was found to be 83.79 percent. The statistical analyses and the closeness of the experimental results and model predictions show the reliability of the regression model and thus, biostimulation and bioaugmentation of indigenous microbial density and activity can reduce remediation period of petroleum hydrocarbon contaminated environment and subsequently the cost of remediation.

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“…Whereas in an experiment on crude-oil-contaminated sand sediment, a C:N:P ratio of 100:25:25 and biosurfactant addition were found to reduce heavy oil fraction at about 30% [48].…”

Section: Nitrogen/ammonium As An Important Macronutrientmentioning

confidence: 89%

Integrated Perspective for Effective Bioremediation

Paliwal

Puranik

Purohit

2011

Appl Biochem Biotechnol

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Identification of factors which can influence the natural attenuation process with available microbial genetic capacities can support the bioremediation which has been viewed as the safest procedure to combat with anthropogenic compounds in ecosystems. With the advent of molecular techniques, assimilatory capacity of an ecosystem can be defined with changing community dynamics, and if required, the essential genetic potential can be met through bioaugmentation. At the same time, intensification of microbial processes with nutrient balancing, expressing and enhancing the degradative capacities, could reduce the time frame of restoration of the ecosystem. The new concept of ecosystems biology has added greatly to conceptualize the networking of the evolving microbiota of the niche that helps in effective application of bioremediation tools to manage pollutants as additional carbon source.

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Bioremediation of Marine Sediments Impacted by Petroleum

Cited by 26 publications

References 14 publications

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

Molecular Mechanisms of Enhanced Bacterial Growth on Hexadecane with Red Clay

Factorial Designs Application to Study Enhanced Bioremediation of Soil Artificially Contaminated with Weathered Bonny Light Crude Oil through Biostimulation and Bioaugmentation Strategy

Integrated Perspective for Effective Bioremediation

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