Bioremediation Efficacy of Extracellular Chromate Reductase from Bacillus amyloliquefaciens (CSB 9) for Detoxification of Hexavalent Chromium

Rath, Bhagwat Prasad; Das, Saroj Kumar; Thatoi, Hrudayanath

doi:10.1007/978-981-13-6846-2_14

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…The treatment with 60 mg/L Cr(VI) presented higher growth compared to the other three treatments. This phenomenon may be due to the bacteria adapting differently to conditions of the environment, depending on the tolerance mechanism that these use (Dhal, et al, 2013;Rath, et al, 2014;Rath, et al, 2019). A similar phenomenon was observed by Huang et al (2020) when evaluating the biosorption capacity of Mn(II) in a concentration range of Mn(II) (0-10000 mg/L) by the B. thuringiensis HM7 strain.…”

Section: Molecular Identification With 16s Rrna Genesupporting

confidence: 56%

“…Concerning B. amyloliquefaciens, Rath et al (2014) indicated that B. amyloliquefaciens isolated from a chromite mine can produce an extracellular enzyme (chromate reductase) that allows the reduction of hexavalent chromium to trivalent chromium, in stress conditions (Rath, et al, 2014). Later, Rath, et al, (2019) report the production of a B. amyloliquefaciens chromate reductase with high stability when subjected to different additives, which positions B. amyloliquefaciens as an ideal candidate for bioremediation of Cr(VI) in a wide range of environmental conditions. In this context, the results obtained in this research give an approximation to the response of these bacterial species could have to different concentrations of chromium Cr(VI) in vivo, even is complex to determine the behavior of bacterial populations in an ecosystem without the help of advanced technology.…”

Section: Bacillus Amyloliquefaciens B Thuringiensis and Paenibacillus Spmentioning

confidence: 99%

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Tolerance and reduction of Cr(VI) by Bacillus amyloliquefaciens, B. thuringiensis and Paenibacillus sp., isolated from Pasto River

Pinta-Melo¹,

Ceballos²,

Ibargüen-Mondragón³

et al. 2022

Lat. Americ. J. of Develop.

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The accumulation of heavy metals such as Cr(VI) in the environment is a growing problem; however, the isolation of the bacteria present in these contaminated sources can provide an alternative for their treatment. In this sense, this research evaluated in vitro the percentage reduction of Cr(VI) and the effect of three concentrations (25, 60, and 100 mg/L of Cr(VI)) on the growth of bacterial isolates E4M3 Bacillus amyloliquefaciens [MK561611], E4M15 Bacillus thuringiensis [MK561610], and Paenibacillus sp. [MK561612]. Statistical analysis determined that 59% of the variation in growth is attributed to the interaction of bacteria and concentration (P <0.05; η2 = 0.59). Likewise, statistically significant differences were observed between bacteria concerning the percentage of reduction of Cr(VI) (P <0.05; η2 = 0.98). The data found on the population density and the percentage of reduction coupled with mathematical approximations are related to the intrinsic metabolic conditions of the bacteria and the selective pressure conditions of the environment where they are found.

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Section: Molecular Identification With 16s Rrna Genesupporting

confidence: 56%

Section: Bacillus Amyloliquefaciens B Thuringiensis and Paenibacillus Spmentioning

confidence: 99%

Tolerance and reduction of Cr(VI) by Bacillus amyloliquefaciens, B. thuringiensis and Paenibacillus sp., isolated from Pasto River

Pinta-Melo¹,

Ceballos²,

Ibargüen-Mondragón³

et al. 2022

Lat. Americ. J. of Develop.

View full text Add to dashboard Cite

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Bioreduction potential of chromate resistant bacteria isolated from chromite mine water of Sukinda, Odisha

Das¹,

Behera

Sudarshan³

et al. 2022

Bioremediation Journal

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Highly Cr(vi)-tolerant Staphylococcus simulans assisting chromate evacuation from tannery effluent

Kalsoom

Batool

Jamil

2021

Green Processing and Synthesis

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Chromium(vi) contaminated sites have been targeted for studying highly chromate-resistant bacteria. From a total of 23 Cr(vi)-resistant bacteria isolated on Luria-Bertani agar medium supplemented with K2CrO4 (1,500 μg/mL), only one strain UT8 (Staphylococcus simulans) was able to tolerate high concentration of chromate, i.e., up to 200 mg/mL in agar medium from tannery effluent. In acetate minimal medium (AMM), it showed maximum tolerance of up to 2,500 μg/mL. Cr(vi) removal potential was 94.6% after 24 h (K2CrO4 1,500 μg/mL). Parametric conditions were optimized in AMM to attain maximum chromate removal. Exopolysaccharides extracted from bacterial cells exposed to chromate exhibited major absorption shifts from 2,500 to 500 cm−1 revealed by Fourier transform infrared spectroscopy. Energy-disperse X-ray spectroscopy further confirmed the adsorption of oxyanions to the bacterial cells. Surface topography of the Cr(vi) treated cells showed transformation into concave shape by scanning electron microscopy. The presence of resistance genes, i.e., chromate reductase (chrR) and class I integrase (intI1), further confirmed tolerance toward chromate. Microarray data analysis of transcriptional gene expression suggested upregulation of cys gene cluster under chromate exposure. Concisely, the present investigations revealed the potential of S. simulans to be an effective candidate for chromate reclamation of wastewater.

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Bioremediation Efficacy of Extracellular Chromate Reductase from Bacillus amyloliquefaciens (CSB 9) for Detoxification of Hexavalent Chromium

Cited by 3 publications

References 12 publications

Tolerance and reduction of Cr(VI) by Bacillus amyloliquefaciens, B. thuringiensis and Paenibacillus sp., isolated from Pasto River

Tolerance and reduction of Cr(VI) by Bacillus amyloliquefaciens, B. thuringiensis and Paenibacillus sp., isolated from Pasto River

Bioreduction potential of chromate resistant bacteria isolated from chromite mine water of Sukinda, Odisha

Highly Cr(vi)-tolerant Staphylococcus simulans assisting chromate evacuation from tannery effluent

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