Biorecycling of shrimp shell by Trichoderma viride for production of antifungal chitinase

Sharaf, Eman F.; El-Sarrany, Abd El-Aziz Qablan; El-Deeb, Mai

doi:10.5897/ajmr12.148

Cited by 12 publications

(4 citation statements)

References 34 publications

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“…Each fungus has a difference in producing enzymes because temperature and pH conditions affect the ability of fungi to hydrolyze substrates. Some research results show that chitin hydrolyzing enzymes generally work optimally in the acidic to neutral pH range (Sharaf et al, 2012). The optimal temperature and pH for the chitinolytic activity of T. viride is 30 o C and pH 5.…”

Section: Effect Of Ph On Chitinase Enzyme Activity Of Trichoderma Asp...mentioning

confidence: 99%

Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum

Rosyida,

Martosudiro,

Muhibuddin

2022

RJLS

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The chitinase enzyme in Trichoderma sp. play an important role in pathogen control. This is because chitin is the main component of the fungal cell wall which the chitinase enzyme can degrade. One Trichoderma sp. isolate was obtained (UBPK6) with the highest percentage of inhibition against Fusarium oxysporum, 76.71%. In addition, UBPK6 isolates also showed the highest bromocresol purple reagent test results (indicating the presence of chitinase enzymes). Chitinase enzyme activity was measured for Trichoderma asperellum isolates. The results showed that the optimum incubation period for enzyme production was the 4th day, with an enzyme activity value of 4.05 U/mL. It indicates that this time is the right time for harvesting enzymes. Furthermore, the effect of pH on the chitinase enzyme activity of Trichoderma asperellum fungus. The test results showed that the optimum value was produced at pH 5 with a value of 3.4 U/mL and decreased afterward. The pH five treatment was the best in inhibiting the growth of pathogens, with an inhibition value of 60.63%. The higher the content of the chitinase enzyme, the higher its ability to degrade damage to the germination of pathogenic spores, thus causing the growth of the pathogen to be inhibited.

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Section: Effect Of Ph On Chitinase Enzyme Activity Of Trichoderma Asp...mentioning

confidence: 99%

Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum

Rosyida,

Martosudiro,

Muhibuddin

2022

RJLS

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“…The inhibition activity between T. harzianum + G. mosseae against the pathogen was evident and could be attributed to their compatibility and synergy, in addition to the toxic and enzymatic capacity which enhance the ability of plant resistance against pathogen (31). Pretransplant inoculation with mycorrhizal fungi can be a way to give efficient strains an immediate spatial advantage over the indigenous fungi which have to compete for root space (32).…”

Section: Isr By G Mosseae and T Harizanum On Cucumber Plants Infected By A Alternata In Plastic Potsmentioning

confidence: 99%

Synergistic interaction of Glomus mosseae T. and Trichoderma harzianum R. in the induction of systemic resistance of Cucumis sativus L. to Alternaria alternata (Fr.) K.

2020

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Due to the various negative impacts of chemical fungicides, the reduction of its applications in agricultural production process is widely recommended. Thus, the need and application of bio-agents in disease control has increased tremendously. The current study aimed at investigating the role of both bio-agents Glomus mosseae (mycorrhizal fungi) and Trichoderma harzianum in protection of Cucumis sativus (cucumber plants) against the fungal pathogen Alternaria alternata which is an opportunistic pathogen and the causal agent of cucumber wilt disease. Results obtained from this work revealed the positive influence of using bio-agents treatments in the reduction of pathogenic effects of A. alternata. The results also showed that G. mosseae and T. harzianum combination had a positive synergistic influence in reducing the detrimental effects of A. alternata ny improving the biomass yield (e.g. fresh and dry weight of root); as well as, on disease severity on C. sativus. Bio-agents (G. mosseae and T. harzianum) increased resistance in C. sativus by raising the production of enzymes catalase and peroxidase. Conclusively, this research revealed that using a multifarious combination of bio-agents significantly (P =.05) increased the efficiency of biological control of A. alternata than using each of them exclusively. Thus, it is recommended that to get an effective result in the control of the pathogen A. alternata in crops as highlighted by the results of this work; a combination of two bio-agents should be used.

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“…The previously optimized crude chitinase secreted by the antagonist T. viride exerted a strong inhibitory activity against the pathogens Alternaria solani, Botrytis cinerea, Penicillium oxalicum and P. italicum (isolated from some spoiled vegetables) using cup plate method, where the first species being the most affected. The optimized production medium (pH 4) contained (g%) 1.5 colloidal chitin, 0.25 peptone, KH2PO4, 0.1; MgSO4.7H2O, 0.05, KCl, 0.05 and 100 μg/ml FeSO4 [14].…”

Section: The Antagonist and The Phytopathogensmentioning

confidence: 99%

“…One ml of the previously prepared optimized crude chitinase [14] with 50 µl Tween 80 was sprayed on the surface of some house insects (cockroach, ant and spider). The insects were left at room temperature in beakers covered with a piece of perforated cloth.…”

Section: Insecticidal Activity Of Trichoderma Viride Crude Chitinase mentioning

confidence: 99%

Mode of Parasitism of the Antagonist Trichoderma Viride towards the Phytopathogen Alternaria Solani

Sharaf

El-Deeb

2018

Journal of Asian Scientific Research

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In a previous work, the optimized crude chitinase of Trichoderma viride exhibited strong inhibitory activity against food spoilage moulds (phytopathogens) Alternaria solani, Botrytis cinerea, Penicillium oxalicum and P. italicum, with the first species being the most affected. Currently, on testing the antagonistic activity of T. viride against the previous phytopathogens, the antagonist exhibited a strong activity particularly towards A. solani. The antagonist displayed more than one mechanism of parasitism on A. solani including penetration of the pathogen hyphae, abnormal pathogen hyphae swelling, lack of conidia formation, conidia malformation as well as degradation of the pathogen cell wall chitin through secretion of chitinase enzyme which is a major cause of antagonism. Fortunately, the present chitinase secreted by Trichoderma viride is found to be a constitutive enzyme. The previously optimized enzyme showed also a powerful insecticidal activity against home insects cockroach, spider and ant where the exoskeleton was softened and hydrolyzed, followed by rapid killing of the insects within 12 minutes. Contribution/ Originality: This study is one of very few studies which have investigated the in vitro mode of parasitism of Trichoderma viride on the food spoilage mold A. solani which exhibited more than one mechanism including hyphae penetration, abnormal hyphae swelling, conidia malformation and lysis of the cell wall chitin.

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Biorecycling of shrimp shell by Trichoderma viride for production of antifungal chitinase

Cited by 12 publications

References 34 publications

Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum

Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum

Synergistic interaction of Glomus mosseae T. and Trichoderma harzianum R. in the induction of systemic resistance of Cucumis sativus L. to Alternaria alternata (Fr.) K.

Mode of Parasitism of the Antagonist Trichoderma Viride towards the Phytopathogen Alternaria Solani

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