Biorecognition of Escherichia coli K88 adhesin for glycated porcine albumin

Sarabia-Sainz, Jose A.; Ramos‐Clamont, Gabriela; Candia-Plata, Ma. María del Carmen; Vázquez–Moreno, Luz

doi:10.1016/j.ijbiomac.2008.11.009

Cited by 16 publications

(24 citation statements)

References 51 publications

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“…However, ETEC K88 bears F4 fimbriae [31], which is described as mannose-resistant in the agglutination test with 0.5% D-mannose [32]. Nevertheless, all three variants of K88 adhesin expressed on the tip of the F4 fimbriae recognize carbohydrate structures expressed on host cell glycoconjugates [33] such as sialoglycoproteins, intestinal mucin-type glycoproteins and neutral glycosphingolipids [34]. Indeed, β-linked galactose residues have been found to be essential component in the attachment of K88 fimbriae to mucus K88 receptors [35,36].…”

Section: Discussionmentioning

confidence: 99%

Effect of Saccharomyces cerevisiae var. Boulardii and beta-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)

Badía

Zanello

Chevaleyre

et al. 2012

Vet Res

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Probiotic and prebiotics, often called "immune-enhancing" feed additives, are believed to deal with pathogens, preventing the need of an immune response and reducing tissue damage. In this study, we investigated if a recently developed β-galactomannan (βGM) had a similar protective role compared to Saccharomyces cerevisiae var. Boulardii (Scb), a proven probiotic, in the context of enterotoxigenic Escherichia coli (ETEC) infection. ETEC causes inflammation, diarrhea and intestinal damage in piglets, resulting in large economic loses worldwide. We observed that Scb and βGM products inhibited in vitro adhesion of ETEC on cell surface of porcine intestinal IPI-2I cells. Our data showed that Scb and βGM decreased the mRNA ETEC-induced gene expression of pro-inflammatory cytokines TNF-α, IL-6, GM-CSF and chemokines CCL2, CCL20 and CXCL8 on intestinal IPI-2I. Furthermore, we investigated the putative immunomodulatory role of Scb and βGM on porcine monocyte-derived dendritic cells (DCs) per se and under infection conditions. We observed a slight up-regulation of mRNA for TNF-α and CCR7 receptor after co-incubation of DC with Scb and βGM. However, no differences were found in DC activation upon ETEC infection and Scb or βGM co-culture. Therefore, our results indicate that, similar to probiotic Scb, prebiotic βGM may protect intestinal epithelial cells against intestinal pathogens. Finally, although these products may modulate DC activation, their effect under ETEC challenge conditions remains to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Effect of Saccharomyces cerevisiae var. Boulardii and beta-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)

Badía

Zanello

Chevaleyre

et al. 2012

Vet Res

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“…The latter has been described as mannose resistant to erythrocyte agglutination test in the presence of D-Mannose (5%) 11 . Nevertheless, the K88 adhesins (ab, ac and ad) bind to β-galactose residues of receptors of syalic glycoprotein and to neutral glycosphingolipids expressed on the surface of the intestinal mucosa 12 . The presence of β-galactose in Scb and βGM may mimic host receptor for K88 adhesin 4 and therefore inhibit ETEC attachement to cell surface.…”

Section: Resultsmentioning

confidence: 99%

Oligosaccharide structure determines prebiotic role of β-galactomannan against Salmonella enterica ser. Typhimurium in vitro

et al. 2013

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Prebiotics and probiotics are considered natural alternatives to dietary antibiotics in animal production. Plant extracts and yeast cell walls are mannose—rich products that can be used as substrate for adhesion of Gram-negative bacteria. We assessed whether the structure of these saccharides is relevant to develop their role as prebiotics and therefore, their suitability to be used as alternatives to antibiotics to prevent intestinal infections in pigs. The prebiotic functionality of β-galactomannan (βGM), mannanoligosaccharide from yeast Saccharomyces cerevisiae (Mannan SC) and monosaccharide D-Mannose were studied in porcine intestinal epithelial cells (IPI-2I) challenged with Salmonella enterica ser. Typhimurium. Results showed that in vitro challenge with Salmonella induces the secretion of proinflammatory cytokine IL6 and chemokine CXCL8 compared with control without infection. Both βGM and Mannan SC, attenuate Salmonella-induced secretion of IL6 and CXCL8. Interestingly, cells treated with D-mannose showed similar levels of proinflammatory IL6 and CXCL8 compared with the control of infection. These data suggest that prebiotic role of βGM is related to its oligosaccharide structure.

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“…Enzyme linked lectin recognition assays (ELLA assays). Recognition of PSA-Lac by biotin-labeled RCA1 lectin (specific for galactose) was done by Enzyme-linked lectin assay (ELLA) according to Sarabia-Sainz et al (2009). Briefly, polystyrene ELISA microtitre plates were coated with 100 µL of diluted solutions of PSA-Lac treatments in 50 mM sodium bicarbonate buffer (pH 9.5).…”

Section: Methodsmentioning

confidence: 99%

“…Piglet mucin isolation. Duodenum sections from nine healthy piglets (17 days old) were obtained and mucins released according to Sarabia-Sainz et al (2009). E. coli K88 growth.…”

Section: Methodsmentioning

confidence: 99%

“…Controlled and limited glycation at this point enables a direct attachment to protein without any prior modification or chemical activation of the reagents (Ledesma-Osuna et al, 2008). Sarabia-Sainz et al (2009) and Ledesma-Osuna et al (2008) demonstrated that lactosylated porcine serum albumin (PSA) neoglycans obtained by Maillard reaction interact with galactose-specific K88 adhesin. This microbial lectin expressed on the Escherichia coli K88 cell surface is responsible for the initial infection of piglets that results in diarrhea.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose.

et al. 2011

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Recently, glyco-therapy is proposed to prevent the interaction of bacterial lectins with host ligands (glycoconjugates). This interaction represents the first step in infection. Neoglycans referred to as PSA-Lac (PSA-Glu (β1-4) Gal) were obtained by conjugation of porcine serum albumin (PSA) with lactose at 80 °C, 100 °C and 120 ºC. Characterization studies of the products showed that PSA could contain 1, 38 or 41 added lactoses, depending on the reaction temperature. These neoglycans were approximately 10 times more glycated than PSA-Lac obtained in previous work. Lactose conjugation occurred only at lysines and PSA-Lac contained terminal galactoses as confirmed by Ricinus communis lectin recognition. Furthermore, Escherichia coli K88+, K88ab, K88ac and K88ad adhesins showed affinity toward all PSA-Lac neoglycans, and the most effective was the PSA-Lac obtained after 100 ºC treatment. In vitro, this neoglycan partially inhibited the adhesion of E. coli K88+ to piglet mucin (its natural ligand). These results provide support for the hypothesis that glycated proteins can be used as an alternative for bioactive compounds for disease prevention.

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Biorecognition of Escherichia coli K88 adhesin for glycated porcine albumin

Cited by 16 publications

References 51 publications

Effect of Saccharomyces cerevisiae var. Boulardii and beta-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)

Effect of Saccharomyces cerevisiae var. Boulardii and beta-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)

Oligosaccharide structure determines prebiotic role of β-galactomannan against Salmonella enterica ser. Typhimurium in vitro

Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose.

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