Bioreactors for Tissue Engineering Purposes

Blose, Kory J.; Krawiec, Jeffrey T.; Weinbaum, Justin S.; Vorp, David A.

doi:10.1016/b978-0-12-398523-1.00013-6

Cited by 10 publications

(5 citation statements)

References 59 publications

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“…are main unit operations in many biochemical processes in which enzymes, microbial, mammalian, or plant cell systems are used for manufacture of a wide range of useful products (Najafpour, 2007;Blose et al, 2014). At laboratory settings, fermentation experiments are often conducted by using shake flasks and the bench-top bioreactors.…”

Section: Bioreactorsmentioning

confidence: 99%

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Arriafdi

Ramachendrin

Alam

2021

MJS

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This paper presents the establishment of a miniature bioreactor platform for anaerobic microbial fermentation processes. It is made from a universal glass bottle and has a working volume of 16 mL. Reactor features included mixing via magnetic stirrer, temperature control via electrical heater and cells optical density (OD) sensing. All sensors and actuators integrated into the reactor were operated using LabVIEW TM (National Instrument, TX, US). The top lid of the bottle was modified to include a 3mm poly(methylmethacrylate) (PMMA) polymer layer where it was machined to provide spaces for fluidic ports and integration of sensors. Each reactor components were sterilized prior to conducting fermentation experiments. Main body of the reactor was made of glass and it was sterilized using the standard heat sterilization method (121 o C for 15 minutes) where else other components were sterilized by exposure to UV light for 2 hours. A series of anaerobic fermentation experiments were conducted in batch mode using S.cerevisiae to evaluate the workability of the system. Fermentation experiments were conducted using inoculum concentration of 2 g•L-1 and starting glucose concentration between 10 g•L-1 and 20 g•L-1. In every experiment, mixing was set to operate at 400 rpm and temperature was adjusted to 30 ± 2 o C. Experiments were carried out until stationary phase was attained. Under these conditions, the best fermentation profile was obtained with glucose concentration of 20 /L where cell specific growth rate was found to be about 0.28 h-1. Bench marking step was also performed where results attained in a miniature bioreactor platform were comparable with the one attained using a 50 mL flask.

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Section: Bioreactorsmentioning

confidence: 99%

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Arriafdi

Ramachendrin

Alam

2021

MJS

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“…Over recent years, multiple approaches have been developed to design suitable platforms ensuring the abovementioned stimulation strategies, and in particular, the use of bioreactor systems. Bioreactor is defined as an in vitro culture system that has been designed to mimic the physiological conditions of the tissue of interest, acting on cell behavior, metabolism, and interaction within constructs in order to achieve the proper tissue structure, organization, and ultimately maturation and function [119]. In this context, several types of bioreactors have been developed and manufactured to mimic the in vivo SKM tissue environment physiology, providing a powerful and reliable model to study cell-ECM interaction during myogenesis and regeneration.…”

Section: Bioreactor Platforms In Skm Tissue Engineering Approachesmentioning

confidence: 99%

Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements

et al. 2020

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Recently, skeletal muscle represents a complex and challenging tissue to be generated in vitro for tissue engineering purposes. Several attempts have been pursued to develop hydrogels with different formulations resembling in vitro the characteristics of skeletal muscle tissue in vivo. This review article describes how different types of cell-laden hydrogels recapitulate the multiple interactions occurring between extracellular matrix (ECM) and muscle cells. A special attention is focused on the biochemical cues that affect myocytes morphology, adhesion, proliferation, and phenotype maintenance, underlining the importance of topographical cues exerted on the hydrogels to guide cellular orientation and facilitate myogenic differentiation and maturation. Moreover, we highlight the crucial role of 3D printing and bioreactors as useful platforms to finely control spatial deposition of cells into ECM based hydrogels and provide the skeletal muscle native-like tissue microenvironment, respectively.

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“…A biological reaction carried out into a vessel and culture aerobic cells are used for conducting enzymatic immobilization [4]. Different types of bio-reactor or fermenters: Continuous Stirred Tank Bioreactor: In a vessel, the time will no longer vary the contents to hold up of micro-organisms and the components will contain some concentration in the fermenter.…”

Section: Types Of Bioreactors or Fermentersmentioning

confidence: 99%

Implementation Bioreactor of Fed-Batch OperatingMode using Biomass Growth Adaptive Control System

Sathyaraj¹,

Levišauskas²,

Urniezius³

2019

ijastems

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This paper work describes the glycerol control system. The operation of the glycerol control system has been analyzed and actual showing intervals determined.The research part describes the research methods: First order time delay models, second order polynomial model, PID control algorithm, Cohen and Coon tuning rules. The goal of present work develop and investigate biomass growth control system in fed-batch operating mode bioreactor. This paper provides the develop and investigate a model for simulation of adaptive control system performance for tracking of specific growth rate at specific set point time trajectories and compare the result with ordinary control system performance indices. In the investigation part of the study, the calculation was made according to three different methods. The mathematical model parameters are obtained using an open loop test.The calculated reaction curve results are compared with experimental results using a second order polynomial method. The experimental results were carried out using an adaptive system and non-adaptive system methods. The results of the two methods were compared and the problem was solved. The tables of calculation with the results and the optimized results graphs are presented. Experimental calculations, programming and modelling performed using MATLAB/SIMULINK software.

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Bioreactors for Tissue Engineering Purposes

Cited by 10 publications

References 59 publications

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Sterilizable Miniature Bioreactor Platform for Anaerobic Fermentation Process

Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements

Implementation Bioreactor of Fed-Batch OperatingMode using Biomass Growth Adaptive Control System

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