Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements

Boso, Daniele; Maghin, Edoardo; Carraro, Eugenia; Giagante, Mattia; Pavan, Piero G.; Piccoli, Martina

doi:10.3390/ma13112483

Cited by 37 publications

(21 citation statements)

References 143 publications

(162 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among many areas, particular interest in hydrogels is observable in tissue engineering. Studies on the hydrogels with potential application in this area of medicine were performed by Boso et al [ 2 ], Mantha et al [ 3 ], and Chung et al [ 4 ]. Interesting investigations were performed by Ilgin et al, who described the synthesis of hydrogels based on N -tert-butylmaleimic acid (TBMAC) and poly( N -isopropylcrylamide) (p(NIPAM)).…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

et al. 2020

View full text Add to dashboard Cite

In this work, synthesis and investigations on chitosan-based hydrogels modified with Aloe vera juice are presented. These materials were synthesized by UV radiation. Investigations involved analysis of chemical structure by FTIR spectroscopy, sorption properties in physiological liquids, strength properties by texture analyzer, surface topography by Atomic Force Microscopy (AFM technique), and in vitro cytotoxicity by MTT test using L929 murine fibroblasts. Particular attention was focused both on determining the impact of the amount and the molecular weight of the crosslinker used for the synthesis as well as on the introduced additive on the properties of hydrogels. It was proven that modified hydrogels exhibited higher swelling ability. Introduced additive affected the tensile strength of hydrogels—modified materials showed 23% higher elongation. The greater amount of the crosslinker used in the synthesis, the more compact the structure, leading to the lower elasticity and lower sorption of hydrogels was reported. Above 95%, murine fibroblasts remained viable after 24 h incubation with hydrogels. It indicates that tested materials did not exhibit cytotoxicity toward these lines. Additionally, materials with Aloe vera juice were characterized by lower surface roughness. Conducted investigations allowed us to state that such modified hydrogels may be considered as useful for biomedical purposes.

show abstract

Section: Introductionmentioning

confidence: 99%

Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Hydrogels based on natural elements are ideal candidates as they elicit a limited inflammatory response, have structurally similar elements to the body and they are highly effective in triggering the skeletal muscle regeneration process [ 136 , 137 ]. Recent strategies have focused on naturally derived hydrogel systems to supply essential biochemical signals required for cell adhesion, proliferation, and myogenic differentiation [ 125 ].…”

Section: Biomaterials For the Transplantation Of Striated Muscle Cellsmentioning

confidence: 99%

Current Strategies for the Regeneration of Skeletal Muscle Tissue

Alarçin

Bal‐Öztürk

Avcı

et al. 2021

IJMS

View full text Add to dashboard Cite

Traumatic injuries, tumor resections, and degenerative diseases can damage skeletal muscle and lead to functional impairment and severe disability. Skeletal muscle regeneration is a complex process that depends on various cell types, signaling molecules, architectural cues, and physicochemical properties to be successful. To promote muscle repair and regeneration, various strategies for skeletal muscle tissue engineering have been developed in the last decades. However, there is still a high demand for the development of new methods and materials that promote skeletal muscle repair and functional regeneration to bring approaches closer to therapies in the clinic that structurally and functionally repair muscle. The combination of stem cells, biomaterials, and biomolecules is used to induce skeletal muscle regeneration. In this review, we provide an overview of different cell types used to treat skeletal muscle injury, highlight current strategies in biomaterial-based approaches, the importance of topography for the successful creation of functional striated muscle fibers, and discuss novel methods for muscle regeneration and challenges for their future clinical implementation.

show abstract

“…An abundance of studies highlighted the opportunity that hydrogels offer as 3D scaffolds for advanced in vitro cell cultures and in vivo tissue replacement [5][6][7][8]. Among the different types of hydrogel that have been developed (synthetic, natural, or mixed hydrogels), decellularized extracellular matrix (ECM)-derived formulations are considered nowadays the best choice to mimic the tissue structure and composition because they consist of a natural microenvironment that is devoid of cells and that facilitates reseeded cell-ECM connections and 3D cellular organization in a similar manner to those of living tissues [9]. As for other organs, decellularized ECM (dECM) from skeletal muscle (SKM) can be transformed into hydrogel, exploiting a collagen-based self-assembly process that is regulated in part by the presence of glycosaminoglycans, proteoglycans, and other ECM proteins [10].…”

Section: Introductionmentioning

confidence: 99%

Porcine Decellularized Diaphragm Hydrogel: A New Option for Skeletal Muscle Malformations

et al. 2021

Self Cite

View full text Add to dashboard Cite

Hydrogels are biomaterials that, thanks to their unique hydrophilic and biomimetic characteristics, are used to support cell growth and attachment and promote tissue regeneration. The use of decellularized extracellular matrix (dECM) from different tissues or organs significantly demonstrated to be far superior to other types of hydrogel since it recapitulates the native tissue’s ECM composition and bioactivity. Different muscle injuries and malformations require the application of patches or fillers to replenish the defect and boost tissue regeneration. Herein, we develop, produce, and characterize a porcine diaphragmatic dECM-derived hydrogel for diaphragmatic applications. We obtain a tissue-specific biomaterial able to mimic the complex structure of skeletal muscle ECM; we characterize hydrogel properties in terms of biomechanical properties, biocompatibility, and adaptability for in vivo applications. Lastly, we demonstrate that dECM-derived hydrogel obtained from porcine diaphragms can represent a useful biological product for diaphragmatic muscle defect repair when used as relevant acellular stand-alone patch.

show abstract

Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements

Cited by 37 publications

References 143 publications

Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

Physicochemical Investigations of Chitosan-Based Hydrogels Containing Aloe Vera Designed for Biomedical Use

Current Strategies for the Regeneration of Skeletal Muscle Tissue

Porcine Decellularized Diaphragm Hydrogel: A New Option for Skeletal Muscle Malformations

Contact Info

Product

Resources

About