Bioreactors for growth of plant roots

Rodríguez-Mendiola, M. A.; Stafford, Angela; Cresswell, R. C.; Arias-Castro, Carlos

doi:10.1016/0141-0229(91)90046-d

Cited by 55 publications

(14 citation statements)

References 10 publications

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“…In contrast with sotolone, some of these flavonoids and sapogenins have been identified in plant cell and tissue cultures of fenugreek (22,24). In a previous study, for instance, we found that hairy roots of fenugreek produce diosgenin in airlift bioreactors and conical flasks, but they did not produce sotolone (7).…”

Section: Introductionmentioning

confidence: 87%

Sotolone Production by Hairy Root Cultures of Trigonella foenum-graecum in Airlift with Mesh Bioreactors

Peraza-Luna

Rodríguez-Mendiola²,

Arias-Castro³

et al. 2001

J. Agric. Food Chem.

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3-Hydroxy-4,5-dimethyl-2(5H)-furanone (sotolone) and 3-amino-4,5-dimethyl-2(5H)-furanone, the postulated precursor of sotolone, were detected in hairy root cultures of Trigonella foenum-graecum (fenugreek) by GC-MS. The hairy root cultures in both conical flasks and airlift with mesh bioreactors were achieved from hypocotyl of seedling by infection with Agrobacterium rhizogenes. In flasks, the mathematical relationship between hairy root growth and conductivity was established and afterward used to evaluate the biomass evolution in bioreactor cultures due to the difficulty of obtaining direct biomass samples from the bioreactor. The GC-MS analyses of ethanolic extracts from hairy roots revealed the presence of two important compounds: sotolone (1.2% of the volatile fraction) and 3-amino-4,5-dimethyl-2(5H)-furanone (17% of the volatile fraction). These results point out that biotechnological production of sotolone in bioreactors is possible. Additionally, these hairy root cultures offer, for the first time, an excellent biological model to study the biosynthetic pathway of sotolone in fenugreek.

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Section: Introductionmentioning

confidence: 87%

Sotolone Production by Hairy Root Cultures of Trigonella foenum-graecum in Airlift with Mesh Bioreactors

Peraza-Luna

Rodríguez-Mendiola²,

Arias-Castro³

et al. 2001

J. Agric. Food Chem.

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“…The possibility of using fast growing cultures of Agrobacterium rhizogenes transformed roots, popularly called hairy roots, for producing such metabolites (Buitelaar et al, 1991;Hilton et al, 1988;Hilton and Rhodes, 1990;Rhodes et al, 1986;Rodriguez-Mendiola et al, 1991;Taya et aL, 1989;Whitney, 1992;Wilson et al, 1987)and study their biosynthesis (Menelaou et al, 1991a,b;Gomez-Barrios et al, 1992) has received a lot of attention.…”

Section: Introductionmentioning

confidence: 99%

A flow cell reactor for the study of growth kinetics of single hairy roots

Flint-Wandel

Hjortsø

1993

Biotechnol Tech

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“…1). In vitro production of diosgenin in this species has already been reported (Khanna and Jain 1973;Brain and Lockwood 1976;Rodriguez-Mendiola et al 1991) but optimization of the hairy root culture conditions has not been previously investigated. Diosgenin and phytosterols are very important as raw materials for the semi-synthesis of steroid hormones.…”

Section: Introductionmentioning

confidence: 99%

Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L.

1997

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Hairy root cultures of Trigonella foenum-graecum L. were established with Agrobacterium rhizogenes strain A 4 . The hairy roots produce diosgenin, an important spirostanol for the semi-synthesis of steroid hormones. Fourteen different liquid media were investigated. The fastest growth was obtained in McCown's woody plant (WP) medium supplemented with 3% sucrose; the highest diosgenin content was observed in half-strength WP medium with 1% sucrose (0.040% dry weight), which represents almost twice the amount detected in the 8-month-old non-transformed roots (0.024%). A time-course study in WP liquid media supplemented with 3% sucrose was undertaken. In these conditions, 17 µg diosgenin/g fresh weight were produced. The influence of cholesterol, medium pH and chitosan on diosgenin production was tested. The addition of 40 mg/l chitosan elevated the diosgenin content to three times that found in non-elicited hairy roots.

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Bioreactors for growth of plant roots

Cited by 55 publications

References 10 publications

Sotolone Production by Hairy Root Cultures of Trigonella foenum-graecum in Airlift with Mesh Bioreactors

Sotolone Production by Hairy Root Cultures of Trigonella foenum-graecum in Airlift with Mesh Bioreactors

A flow cell reactor for the study of growth kinetics of single hairy roots

Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L.

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