Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L.

Merkli, Alain; Christen, Philippe; Kapétanidis, I.

doi:10.1007/s002990050292

Cited by 20 publications

(25 citation statements)

References 10 publications

(10 reference statements)

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“…as obtained from 20-d-old root culture. Similar to the present results, better growth and higher diosgenin accumulation in Trigonella foenum-graecum hairy roots (Merkli et al 1997) was observed in lower pH (5.0 and 4.8 before and after autoclaving, respectively) in comparison with that grown in higher pH such as 5.5 and 5.9. Although several investigations have been made on influence of pH on growth and secondary metabolite formation in hairy roots of different plant species (Mukundan and Hjorsto 1991), the results regarding this aspect are not clear.…”

Section: ⎯⎯⎯⎯supporting

confidence: 93%

Hairy root culture of Plumbago indica as a potential source for plumbagin

Gangopadhyay

Sircar²,

Mitra³

et al. 2008

Biol. plant.

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Hairy roots of Plumbago indica were established at high frequency (90 %) by infecting leaf explants with Agrobacterium rhizogenes strain ATCC 15834. The axenic root cultures were established under darkness in hormonefree liquid Murashige and Skoog medium containing 3 % sucrose. The highest plumbagin content was found to accumulate in roots at their exponential phase of growth. A low pH (4.6) and a low concentration of sucrose (1 %) were beneficial for root growth in darkness, while pH 5.6 and 3 % sucrose under continuous irradiance enhanced plumbagin accumulation in roots up to 7.8 mg g -1 (d.m.). Direct shoot regeneration from hairy root culture was also achieved under continuous irradiance, thus indicated an easy way of obtaining transformed P. indica plants.Additional key words: Agrobacterium rhizogenes, transformation, organogenesis. ⎯⎯⎯⎯Plumbagin, a naphthoquinone compound occurring mainly in Plumbago species was well known for its use in traditional medicines. Among all Plumbago species, (family Plumbaginaceae), Plumbago indica (syn. rosea) is the best source for harvesting plumbagin (Mallavadhani et al. 2002). According to several current reports (Chetia and Handique 2000) Plumbago indica becomes rare in several parts of India.The objective of this work was to establish a new source for harvesting plumbagin without sacrificing the whole plant. As a result, an attempt was made to produce plumbagin in vitro from hairy root cultures of P. indica, established by genetic transformation using wild strain of Agrobacterium rhizogenes for the first time. The advantage of hairy root culture lies on the fact that these cultures generally grow faster and usually have higher amount of secondary metabolites in comparison to cell suspension culture and even in some cases higher than in intact plant roots (Allan et al. 2002). Growth of hairy roots can be scaled up by using bioreactors, hence are exploitable for commercial synthesis of plant derived natural products as evident from very recent work on anthraquinone production by hairy root culture of Rhamnus fallax (Rosić et al. 2006).In the present study Agrobacterium rhizogenes strain ATCC 15834 was used to induce hairy roots of Plumbago indica L. The bacterial culture was maintained in solid Agrobacterium Broth (AB) minimal medium by routine transfer and prior to use in infecting explants, it was cultured in liquid AB medium on an orbital shaker at 28 ± 2 °C for 3 -6 d. In 1-month-old axenic cultures of Plumbago indica L., bacterial infection was made on some selected sites of the stem and leaves by making wounds with a sterile needle loaded with bacterial suspension, showing absorbance (A 600 ) 0.5. Frequency of successful transformation and days taken for first appearance of hairy roots from leaf and stem explants were recorded.Hairy roots arising from the infected sites were cut at the tips and transferred to the MS solid medium (gelled with 0.8 % agar) containing 3 % sucrose and 250 mg dm -3 cefotaxime (cefotaxime sodium, ALKEM, India) and maintained under darkness ...

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Section: ⎯⎯⎯⎯supporting

confidence: 93%

Hairy root culture of Plumbago indica as a potential source for plumbagin

Gangopadhyay

Sircar²,

Mitra³

et al. 2008

Biol. plant.

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“…Importance of fenugreek seeds pretreatment SC-CO 2 extraction of fenugreek seeds without both steps of pretreatment (defatting and hydrolysis) showed that diosgenin was not present in the collected extract. Importance of hydrolysis was also reported in literature [15][16][17][18] and this study proved that hydrolysis was the main step for pretreatment of fenugreek seeds necessary for diosgenin isolation by SC-CO 2 extraction.…”

Section: Resultssupporting

confidence: 67%

“…Seeds contain 1-2% steroidal sapogenins, mainly diosgenin contained in the plant as steroidal saponin glycoside. Generally, the extraction methods of diosgenin from fenugreek seeds require pretreatment of the plant including defatting as the first step, followed by acid hydrolysis with aim to prepare corresponding hydro-isolate [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology

Bogdanović

Tadić

Stamenić

et al. 2016

The Journal of Supercritical Fluids

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“…This evidence suggests that the biosynthesis of secondary metabolite increased during the stationary phase of growth. Similar observations have been noted for other hairy root cultures (Bhadra and Shanks, 1997;Merkli et al, 1997;Sasaki et al, 1998;Kittipongpatana et al, 2002). Increased production of secondary metabolite at the stationary phase might be come from the stress of tissue due to the change of environment such as undernourishment.…”

Section: Resultssupporting

confidence: 72%

Sennosides A and B Production by Hairy Roots of Senna alata (L.) Roxb.

Putalun

Pimmeuangkao

De-Eknamkul

et al. 2006

Zeitschrift Für Naturforschung C

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Hairy roots of Senna alata transformed with Agrobacterium rhizogenes, strain ATCC 15834 were induced and grown in half-strength Murashige and Skoog (MS) medium. Effects of sucrose contents and hormones on the growth and sennosides A, B production were investigated. Hairy roots cultured on hormone-free half-strength MS medium containing 5% sucrose under dark condition mostly stimulated the growth of hairy roots and increased the content of sennosides A and B yielding (169 ð 4) and (34 ð 3) μg g Ð1 dry wt, respectively.

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Production of diosgenin by hairy root cultures of Trigonella foenum-graecum L.

Cited by 20 publications

References 10 publications

Hairy root culture of Plumbago indica as a potential source for plumbagin

Hairy root culture of Plumbago indica as a potential source for plumbagin

Supercritical carbon dioxide extraction of Trigonella foenum-graecum L. seeds: Process optimization using response surface methodology

Sennosides A and B Production by Hairy Roots of Senna alata (L.) Roxb.

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