Bioreactor studies on the endophytic fungus<i>Entrophospora infrequens</i>for the production of an anticancer alkaloid camptothecin

Amna, Touseef; Puri, S. C.; Verma, Vivek; Sharma, Jai P.; Khajuria, Rajinder K; Musarrat, Javed; Spiteller, Michael; Qazi, Ghulam N.

doi:10.1139/w05-122

Cited by 160 publications

(56 citation statements)

References 14 publications

(12 reference statements)

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“…In continuation of our previous work (39,2,3,40) on microbial chemistry of endophytic culture, herein, we demonstrate the outcome of media manipulations and precursor's treatment on the production of CPT from RJMEF001.…”

Section: Anma T E T a Lsupporting

confidence: 54%

Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus

Amna¹,

Amina²,

Sharma³

et al. 2012

Braz. J. Microbiol.

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We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but fungal endopytes may be an alternative source of production. In present study we have observed the effect of different nutrient combinations and precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid and leucine) on the accumulation of camptothecin by endophytic fungus Entrophospora infrequens. The precursors were fed either alone or in combinations (tryptophan and geraniol, tryptophan and citral, tryptophan and mevalonic acid, tryptophan and leucine). The highest camptothecin content was observed in the range of 503 ± 25µg/100g dry cell mass in Sabouraud medium.Camptothecin content in the medium was increased by 2.5 folds by the presence of tryptophan and leucine whereas the production with trytophan was also significantly different from other treatments. Furthermore, the effect of fungal camptothecin on the morphology of human cancer cell lines was also studied. The treated cells showed reduction in size, condensation of nucleus and the protoplasmic extensions were reduced. All these characteristics are found in apoptotic cells.

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Section: Anma T E T a Lsupporting

confidence: 54%

Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus

Amna¹,

Amina²,

Sharma³

et al. 2012

Braz. J. Microbiol.

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“…It is used for recurrent colon cancer and has unusual activity against lung, ovarian and uterine cancers. 72 Colon cancer is the second leading cause of cancer fatalities in the United States and the third most common cancer among US citizens. Camptothecin is known commercially as Camptosar and Campto and achieved sales of $1 billion in 2003.…”

Section: Antineoplastic Drugsmentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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where he has contributed in a major way to the reputation of this department for many years. He also served as an Adjunct Professor at the University of Geneva. Among Julian's areas of study and accomplishment are fungal toxins including α-sarcin, chemical synthesis of triterpenes, mode of action of streptomycin and other aminoglycoside antibiotics, biochemical mechanisms of antibiotic resistance in clinical isolates of bacteria harboring resistance plasmids, their origins and evolution, secondary metabolism of microorganisms, structure and function of bacterial ribosomes, antibiotic resistance mutations in yeast ribosomes, cloning of resistance genes from an antibiotic-producing microbe, gene cloning for industrial purposes, engineering of herbicide resistance in useful crops, bleomycin-resistance gene in clinical isolates of Staphylococcus aureus and many other topics. He has been an excellent teacher, lecturing in both English and French around the world, and has organized international courses. Julian has also served on the NIH study sections, as Editor for several international journals, and was one of the founders of the journal Plasmid. We expect the impact of Julian's accomplishments to continue into the future.

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“…In view of the low concentration of camptothecin in tree roots and poor yield from chemical synthesis, the fungal fermentation is very promising for industrial production of camptothecin. It is used for recurrent colon cancer and has unusual activity against lung, ovarian, and uterine cancer [ 57 ]. Colon cancer is the second-leading cause of cancer fatalities in the USA and the third most common cancer among US citizens.…”

Section: Anticancer Drugsmentioning

confidence: 99%

Valuable Secondary Metabolites from Fungi

Demain

2014

Fungal Biology

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Bioreactor studies on the endophytic fungusEntrophospora infrequensfor the production of an anticancer alkaloid camptothecin

Cited by 160 publications

References 14 publications

Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus

Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus

Production of valuable compounds by molds and yeasts

Valuable Secondary Metabolites from Fungi

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