Biopsy of Human Morula-Stage Embryos: Outcome of 215 IVF/ICSI Cycles with PGS

Zakharova, E. E.; Zaletova, V.; Krivokharchenko, Alexander

doi:10.1371/journal.pone.0106433

Cited by 38 publications

(28 citation statements)

References 42 publications

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“…The etiology of improved live birth rate following blastocyst transfer is thought to be multifactorial. The increased implantation potential of blastocysts may be due in large part to the higher likelihood of euploid embryos to blastulate by day 5 and then survive post-transfer [18,19]. Moreover, evidence suggests that there may be improved synchronicity between embryo development and the endometrium on day 5.…”

Section: Discussionmentioning

confidence: 99%

Building a model to increase live birth rate through patient-specific optimization of embryo transfer day

Goldman

Kaser

Missmer

et al. 2016

J Assist Reprod Genet

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Purpose Many practices are moving away from cleavagestage transfer in favor of blastocyst transfer. The purpose of this study is to evaluate how the overall live birth rate for fresh IVF cycles may increase by optimizing the day of transfer for each patient. Methods This is a retrospective cohort study of 1225 first fresh autologous IVF cycles performed between May 2012 and November 2013. Stepwise logistic regression was used to determine characteristics associated with live birth following cleavage-stage versus blastocyst transfer. The optimal transfer day (i.e., the day that maximized the odds of live birth) was determined for each patient, and the actual live birth rate was compared with the projected rate had each patient undergone transfer on her optimal day. Results With transfer on the optimal day for each patient, the overall birth rate would have increased from its actual value of 34.8 % to a projected 43.0 %, a 24 % increase. The majority of this increase (21 %) was due to optimization of patients who underwent cleavage-stage transfer but had a higher projected birth rate from blastocyst transfer. These patients were older (37.8 versus 36.0 years, p < 0.01) and had more follicles ≥18 mm than patients who should have remained with a cleavage-stage transfer. Conclusions A model can be built enabling patient-specific identification of optimal transfer day; within this discovery cohort, such optimization was estimated to increase live birth following a fresh transfer by 24 %. This study suggests blastocyst transfer should be more widely offered; however, there remain patients for whom a cleavage-stage transfer may yield better outcomes.

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Section: Discussionmentioning

confidence: 99%

Building a model to increase live birth rate through patient-specific optimization of embryo transfer day

Goldman

Kaser

Missmer

et al. 2016

J Assist Reprod Genet

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“…Reports on neonatal outcomes and obstetrical complications of PGT pregnancies are limited. [76][77][78][79][80][81][82] Some studies have evaluated the effects of embryo biopsy without distinction between PGT-M and PGT-A. 77,80 It is necessary to consider that the technical procedures for PGT-M and PGT-A are similar, while the indication for these techniques is not.…”

Section: Obstetrical and Perinatal Outcomesmentioning

confidence: 99%

Assessment of pre‐implantation genetic testing for embryo aneuploidies: A SWOT analysis

et al. 2019

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The recently re‐named pre‐implantation genetic testing for determining embryo aneuploidies (PGT‐A) is presently very popular although its acceptance by the scientific community is controversial. This approach still encounters drawbacks. This paper uses a SWOT (strengths, weaknesses, opportunities and threats) analysis to discuss salient points to be considered when examining the pre‐implantation genetic testing (PGT‐A) strategy to gather information from a range of perspectives. One of the strengths associated with the procedure is represented by an increase in implantation rate although data from the highest level of evidence do not support an increase in cumulative pregnancy rates. The current difficulty in the management of mosaicisms represents a weakness of PGT‐A. The application of the strategy represents an opportunity to favor the single embryo transfer while other advantages, such as reduction of time to pregnancy and emotional distress are controversial. Potential important threats, at present still undefined, are represented by the biopsy‐related damage to the blastocyst and the impact on neonatal and long‐term outcomes.

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“…Preimplantation genetic diagnosis (PGD) has become a widely accepted and routine technique for testing chromosomal integrity during in vitro fertilization (IVF) procedures, and has been successfully applied to high-risk couples for more than two decades [1,2]. During the development of PGD, polar bodies, blastomeres, and trophectoderms (TE) have been used as biopsy material for analysis.…”

Section: Introductionmentioning

confidence: 99%

Detection and analysis of DNA material in human blastocoel fluid

Shangguan¹,

He²,

Li³

et al. 2017

Biomed Genet Genomics

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Preimplantation genetic diagnosis (PGD) is becoming a widely-accepted technique during in vitro fertilization (IVF). However, a disadvantage of PGD is the invasive biopsy methods used to sample embryonic cells or polar bodies. Recent studies have found that genetic material can be detected in blastocoel fluid (BF) and culture medium. In our study, BF and trophectoderm (TE) cells were simultaneously collected from the same donated human blastula. To generate enough DNA for analysis, we used multiple displacement amplification (MDA) based whole genome amplification (WGA). MDA-WGA samples were probed with primers designed to identify spinal muscular atrophy (SMA) and phenylpropionate ketoneuria (PKU), plus the Y chromosome sex-determining region (SRY). This demonstrated that DNA fragments were present in each of the TE and BF samples (7/7). The positive PCR amplification rates for SMA, PKU, SRY and β-actin in BF were 42.9% (3/7), 60% (3/5), 42.9% (3/7) and 71.4% (5/7) respectively, but the positive rate of amplification with TE samples was 100% (7/7), 100% (7/7), 71.43% (5/7) and 100% (7/7). After sequencing, identical alleles were found in matched BF and TE samples. In summary, BF-DNA could be detected using MDA-WGA and PCR, and sequences in PCR positive samples were identical in matched BF-DNA and TE samples.

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Biopsy of Human Morula-Stage Embryos: Outcome of 215 IVF/ICSI Cycles with PGS

Cited by 38 publications

References 42 publications

Building a model to increase live birth rate through patient-specific optimization of embryo transfer day

Building a model to increase live birth rate through patient-specific optimization of embryo transfer day

Assessment of pre‐implantation genetic testing for embryo aneuploidies: A SWOT analysis

Detection and analysis of DNA material in human blastocoel fluid

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